Taras Vasylyshyn, Vitalii Patsula, Oleksandr Shapoval, Ognen Pop-Georgievski, Solomiya Paryzhak, Tetiana Dumych, Victoria Serhiyenko, Nadia Skorokhyd, Olga Klyuchivska, Rafał Konefał, Jiřina Hromádková, Lucia Machová Urdziková, Dana Mareková, Aleš Benda, Pavla Jendelová, Rostyslav Stoika, Daniel Horák
Upconverting nanoparticles (UCNPs) have attracted much attention in nanomedicine due to their ability to upconvert photons. However, their adverse effects hinder the biomedical applications. In this paper, bisphosphonate-modified poly(isobutylene-alt-maleic acid)-graft-poly(N,N-dimethylacrylamide)-coated NaYF4:Yb,Er,Pr UCNPs (UCNP@PIMAPDMA) nanoparticles were designed, which exhibited luminescence emission simultaneously in the visible and NIR-II regions. The developed UCNPs were characterized by a range of physicochemical methods, including transmission electron and energy dispersive microscopy (TEM and EDAX), dynamic light scattering (DLS), X-ray diffraction analysis (XRD), spectrofluorometry, X-ray photoelectron spectroscopy (XPS), and so forth. The UCNP@PIMAPDMA nanoparticles were also evaluated in cell cultures and experimental animals. The particles showed good biocompatibility with cultured human embryonic kidney HEK293 cells commonly used in toxicological studies. Neat UCNPs were cytotoxic towards these cells, which was confirmed by measuring their viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Blood serum proteins adhered to the surface of UCNP@PIMAPDMA particles, forming a protein corona that may contribute to particle biosafety. After intravenous injection of these particles into laboratory mice, there were no statistically significant changes in body mass of the treated animals. Also, no big adverse effects on blood cell profile, enzymatic and metabolic markers of hepatotoxicity and nephrotoxicity were observed. Finally, the application potential of UCNP@PIMAPDMA nanoparticles was confirmed by successfully imaging the cytoplasm of rat mesenchymal stem cells and rat C6 glioblastoma cells using laser scanning confocal microscopy.
{"title":"Bisphosphonate-Modified Polymer-Coated NaYF4:Yb,Er,Pr Upconverting Nanoparticles for Cell Imaging: Synthesis, Physicochemical Characterization and Biosafety","authors":"Taras Vasylyshyn, Vitalii Patsula, Oleksandr Shapoval, Ognen Pop-Georgievski, Solomiya Paryzhak, Tetiana Dumych, Victoria Serhiyenko, Nadia Skorokhyd, Olga Klyuchivska, Rafał Konefał, Jiřina Hromádková, Lucia Machová Urdziková, Dana Mareková, Aleš Benda, Pavla Jendelová, Rostyslav Stoika, Daniel Horák","doi":"10.1002/jbmb.70011","DOIUrl":"10.1002/jbmb.70011","url":null,"abstract":"<p>Upconverting nanoparticles (UCNPs) have attracted much attention in nanomedicine due to their ability to upconvert photons. However, their adverse effects hinder the biomedical applications. In this paper, bisphosphonate-modified poly(isobutylene-<i>alt</i>-maleic acid)-<i>graft</i>-poly(<i>N,N</i>-dimethylacrylamide)-coated NaYF<sub>4</sub>:Yb,Er,Pr UCNPs (UCNP@PIMAPDMA) nanoparticles were designed, which exhibited luminescence emission simultaneously in the visible and NIR-II regions. The developed UCNPs were characterized by a range of physicochemical methods, including transmission electron and energy dispersive microscopy (TEM and EDAX), dynamic light scattering (DLS), X-ray diffraction analysis (XRD), spectrofluorometry, X-ray photoelectron spectroscopy (XPS), and so forth. The UCNP@PIMAPDMA nanoparticles were also evaluated in cell cultures and experimental animals. The particles showed good biocompatibility with cultured human embryonic kidney HEK293 cells commonly used in toxicological studies. Neat UCNPs were cytotoxic towards these cells, which was confirmed by measuring their viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Blood serum proteins adhered to the surface of UCNP@PIMAPDMA particles, forming a protein corona that may contribute to particle biosafety. After intravenous injection of these particles into laboratory mice, there were no statistically significant changes in body mass of the treated animals. Also, no big adverse effects on blood cell profile, enzymatic and metabolic markers of hepatotoxicity and nephrotoxicity were observed. Finally, the application potential of UCNP@PIMAPDMA nanoparticles was confirmed by successfully imaging the cytoplasm of rat mesenchymal stem cells and rat C6 glioblastoma cells using laser scanning confocal microscopy.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"114 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbmb.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145781351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Panagiotis Ntakos, Christos Kalligeros, Konstantinos Chouzouris, Vasilios Gakos, Athanasios F. Foukas, Athanasios Armakolas, Olga D. Savvidou, Panayiotis J. Papagelopoulos, Vasilios Spitas
This study investigated the biomechanical efficacy of conventional and 3D-printed scaffold-augmented fixation methods for a large 6 cm osseous femoral defect. Finite element analyses were conducted to compare four conventional techniques: single plate, intramedullary nail, combined plate and nail, and double plate. These were then evaluated with the addition of three porous Ti-6Al-4V scaffold designs (Weaire–Phelan, Diamond, and Voronoi) with 70% porosity. Models were subjected to peak physiological loading from gait, simulating a 106 kg patient. Performance was assessed based on implant stress and the volume fraction of the fracture callus experiencing osteogenic strains (0.005%–2.5%). Results showed that conventional single-implant methods were mechanically insufficient; the single plate failed at 20% of the physiological load and the nail at 90%. These methods also produced suboptimal osteogenic environments, with an osteogenic volume fraction < 16%. In contrast, combined conventional methods (plate and nail, double plate) withstood 100% of the load with significantly lower stresses and promoted highly osteogenic environments, with an osteogenic volume fraction > 95%. The integration of 3D-printed scaffolds transformed the single-implant constructs, enabling them to withstand 100% physiological load and increasing their osteogenic volume fraction to over 90%. Scaffolds also substantially reduced stress on the primary implants in all configurations. The plate and nail fixation augmented with a scaffold emerged as the most robust strategy, reducing conventional implant stresses to approximately 140 MPa while maintaining an exceptional osteogenic volume fraction > 99%. These findings highlight the quantitative potential of 3D-printed scaffolds to improve treatment outcomes for large bone defects.
{"title":"Finite Element Analysis of Conventional Fixation and 3D-Printed Scaffold Integration for Treating Large Osseous Femoral Defects","authors":"Panagiotis Ntakos, Christos Kalligeros, Konstantinos Chouzouris, Vasilios Gakos, Athanasios F. Foukas, Athanasios Armakolas, Olga D. Savvidou, Panayiotis J. Papagelopoulos, Vasilios Spitas","doi":"10.1002/jbmb.70010","DOIUrl":"https://doi.org/10.1002/jbmb.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the biomechanical efficacy of conventional and 3D-printed scaffold-augmented fixation methods for a large 6 cm osseous femoral defect. Finite element analyses were conducted to compare four conventional techniques: single plate, intramedullary nail, combined plate and nail, and double plate. These were then evaluated with the addition of three porous Ti-6Al-4V scaffold designs (Weaire–Phelan, Diamond, and Voronoi) with 70% porosity. Models were subjected to peak physiological loading from gait, simulating a 106 kg patient. Performance was assessed based on implant stress and the volume fraction of the fracture callus experiencing osteogenic strains (0.005%–2.5%). Results showed that conventional single-implant methods were mechanically insufficient; the single plate failed at 20% of the physiological load and the nail at 90%. These methods also produced suboptimal osteogenic environments, with an osteogenic volume fraction < 16%. In contrast, combined conventional methods (plate and nail, double plate) withstood 100% of the load with significantly lower stresses and promoted highly osteogenic environments, with an osteogenic volume fraction > 95%. The integration of 3D-printed scaffolds transformed the single-implant constructs, enabling them to withstand 100% physiological load and increasing their osteogenic volume fraction to over 90%. Scaffolds also substantially reduced stress on the primary implants in all configurations. The plate and nail fixation augmented with a scaffold emerged as the most robust strategy, reducing conventional implant stresses to approximately 140 MPa while maintaining an exceptional osteogenic volume fraction > 99%. These findings highlight the quantitative potential of 3D-printed scaffolds to improve treatment outcomes for large bone defects.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145739418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamidreza Dastjerd, Alireza Badiei, Tabassom Hooshmand, Tahereh S. Jafarzadeh Kashi
The objective of this study was to optimize the composition and loading of silver-doped mesoporous silica nanoparticles (Ag/MSNs) within the poly(methyl methacrylate) (PMMA) denture base to enhance mechanical properties, antifungal activity, and maintain biocompatibility. Silica nanoparticles (SBA-15) were synthesized and functionalized with either diaminosilane (PC1200) or gamma-methoxysilane (γ-MPTS) coupling agents prior to silver impregnation. The functionalized and unmodified SBA-15 were then loaded with silver nanoparticles via chemical reduction. These Ag/MSN-modified PMMA composites were fabricated at different nanoparticle loadings (0.1, 0.5, 1, and 1.5 wt%) and characterized for their physical, mechanical, antimicrobial, and biocompatibility properties. The incorporation of Ag/MSNs significantly improved the mechanical properties and antimicrobial activity of the PMMA-based dental prosthesis material without compromising biocompatibility. The modification of Ag/MSNs particles by both types of coupling agents fortified the material's mechanical properties, sustained water solubility and release of silver nanoparticles, thereby providing better biocompatibility for the synthesized nanocomposites. This phenomenon was more pronounced in the amino-silane coupling agents equipped with diamine groups. The findings of this study demonstrated that an increase in the concentration of Ag/MSNs particles in the PMMA polymers up to 1.5 wt% resulted in the deterioration of the mechanical properties, reducing water sorption and solubility, but enhancement of antifungal activity. A concentration of 0.5 wt% silver-doped mesoporous silica nanoparticles modified by amino-silane or gamma-methoxysilane coupling agents can be considered the optimal concentration for incorporation into PMMA denture bases, resulting in enhanced mechanical and antifungal properties while preserving biocompatibility.
{"title":"Enhanced Mechanical and Antifungal Properties of Polymethyl Methacrylate Denture Bases by Incorporation of Silver-Doped Mesoporous Silica Nanocomposites Modified by Two Coupling Agents","authors":"Hamidreza Dastjerd, Alireza Badiei, Tabassom Hooshmand, Tahereh S. Jafarzadeh Kashi","doi":"10.1002/jbmb.70008","DOIUrl":"10.1002/jbmb.70008","url":null,"abstract":"<div>\u0000 \u0000 <p>The objective of this study was to optimize the composition and loading of silver-doped mesoporous silica nanoparticles (Ag/MSNs) within the poly(methyl methacrylate) (PMMA) denture base to enhance mechanical properties, antifungal activity, and maintain biocompatibility. Silica nanoparticles (SBA-15) were synthesized and functionalized with either diaminosilane (PC1200) or gamma-methoxysilane (γ-MPTS) coupling agents prior to silver impregnation. The functionalized and unmodified SBA-15 were then loaded with silver nanoparticles via chemical reduction. These Ag/MSN-modified PMMA composites were fabricated at different nanoparticle loadings (0.1, 0.5, 1, and 1.5 wt%) and characterized for their physical, mechanical, antimicrobial, and biocompatibility properties. The incorporation of Ag/MSNs significantly improved the mechanical properties and antimicrobial activity of the PMMA-based dental prosthesis material without compromising biocompatibility. The modification of Ag/MSNs particles by both types of coupling agents fortified the material's mechanical properties, sustained water solubility and release of silver nanoparticles, thereby providing better biocompatibility for the synthesized nanocomposites. This phenomenon was more pronounced in the amino-silane coupling agents equipped with diamine groups. The findings of this study demonstrated that an increase in the concentration of Ag/MSNs particles in the PMMA polymers up to 1.5 wt% resulted in the deterioration of the mechanical properties, reducing water sorption and solubility, but enhancement of antifungal activity. A concentration of 0.5 wt% silver-doped mesoporous silica nanoparticles modified by amino-silane or gamma-methoxysilane coupling agents can be considered the optimal concentration for incorporation into PMMA denture bases, resulting in enhanced mechanical and antifungal properties while preserving biocompatibility.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tabitha Derr, Cemile Basgul, Paul DeSantis, Ryan M. Bock, Steven M. Kurtz
Silicon nitride (Si3N4) is reported to exhibit antibacterial properties and support osteoblast maturation, while polyetherketoneketone (PEKK) is considered to potentially have antibacterial and osseointegrative properties while offering favorable manufacturability through extrusion-based additive manufacturing compared to traditional ceramics manufacturing. Incorporating silicon nitride into PEKK is hypothesized to enhance its bioactivity while maintaining processability, making Si3N4-PEKK composites promising for medical implants. Our objective was to determine optimal fused filament fabrication (FFF) parameters for PEKK and Si3N4-PEKK. Taguchi optimization (L9 array, n = 5) was performed on PEKK and 15 vol.% Si3N4-PEKK to assess the impact of printing parameters (layer height: 0.1, 0.2, and 0.3 mm; nozzle temperature (PEKK/Si3N4-PEKK): 340/380, 370/400, and 400/420; bed temperature: 130°C, 150°C, and 170°C; and chamber temperature: 110°C, 130°C, and 150°C) on ultimate tensile strength (UTS). Z-directional tensile specimens were printed on a medical FFF printer. Specimens underwent tensile testing according to ASTM D638. Signal/noise ratios for UTS were calculated and ANOVA (Minitab 21.4.2) was used to assess statistical significance (p < 0.05). Layer height had the greatest impact on UTS for both PEKK and Si3N4-PEKK. Optimal nozzle and chamber temperatures were 400°C and 130°C, respectively, while the optimal layer height was 0.1 mm for both materials. The optimal bed temperature for PEKK and Si3N4-PEKK was 150°C and 170°C, respectively. For PEKK, differences in all parameters were significant except for bed temperature, while for Si3N4-PEKK all parameters were significant except for nozzle temperature. The specimens with optimum statistically significant parameters showed the highest UTS for both PEKK (91 ± 2 MPa) and Si3N4-PEKK (76 ± 3 MPa). Layer height is the most influential printing variable for both PEKK and Si3N4-PEKK. The optimal PEKK printing condition has a comparable UTS, while Si3N4-PEKK achieved 84% of the injection-molded value for neat PEKK.
{"title":"Taguchi Optimization of Additively Manufactured PEKK and Silicon Nitride Loaded PEKK for Medical Device Applications","authors":"Tabitha Derr, Cemile Basgul, Paul DeSantis, Ryan M. Bock, Steven M. Kurtz","doi":"10.1002/jbmb.70009","DOIUrl":"10.1002/jbmb.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>Silicon nitride (Si<sub>3</sub>N<sub>4</sub>) is reported to exhibit antibacterial properties and support osteoblast maturation, while polyetherketoneketone (PEKK) is considered to potentially have antibacterial and osseointegrative properties while offering favorable manufacturability through extrusion-based additive manufacturing compared to traditional ceramics manufacturing. Incorporating silicon nitride into PEKK is hypothesized to enhance its bioactivity while maintaining processability, making Si<sub>3</sub>N<sub>4</sub>-PEKK composites promising for medical implants. Our objective was to determine optimal fused filament fabrication (FFF) parameters for PEKK and Si<sub>3</sub>N<sub>4</sub>-PEKK. Taguchi optimization (L9 array, <i>n</i> = 5) was performed on PEKK and 15 vol.% Si<sub>3</sub>N<sub>4</sub>-PEKK to assess the impact of printing parameters (layer height: 0.1, 0.2, and 0.3 mm; nozzle temperature (PEKK/Si<sub>3</sub>N<sub>4</sub>-PEKK): 340/380, 370/400, and 400/420; bed temperature: 130°C, 150°C, and 170°C; and chamber temperature: 110°C, 130°C, and 150°C) on ultimate tensile strength (UTS). Z-directional tensile specimens were printed on a medical FFF printer. Specimens underwent tensile testing according to ASTM D638. Signal/noise ratios for UTS were calculated and ANOVA (Minitab 21.4.2) was used to assess statistical significance (<i>p</i> < 0.05). Layer height had the greatest impact on UTS for both PEKK and Si<sub>3</sub>N<sub>4</sub>-PEKK. Optimal nozzle and chamber temperatures were 400°C and 130°C, respectively, while the optimal layer height was 0.1 mm for both materials. The optimal bed temperature for PEKK and Si<sub>3</sub>N<sub>4</sub>-PEKK was 150°C and 170°C, respectively. For PEKK, differences in all parameters were significant except for bed temperature, while for Si<sub>3</sub>N<sub>4</sub>-PEKK all parameters were significant except for nozzle temperature. The specimens with optimum statistically significant parameters showed the highest UTS for both PEKK (91 ± 2 MPa) and Si<sub>3</sub>N<sub>4</sub>-PEKK (76 ± 3 MPa). Layer height is the most influential printing variable for both PEKK and Si<sub>3</sub>N<sub>4</sub>-PEKK. The optimal PEKK printing condition has a comparable UTS, while Si<sub>3</sub>N<sub>4</sub>-PEKK achieved 84% of the injection-molded value for neat PEKK.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Huang, J. Wu, D. Liu, and P. Gao, “Advances in Magnesium Metal and Its Alloys for Promoting Angiogenesis,” Journal of Biomedical Materials Research Part B: Applied Biomaterials 113, no. 11 (2025): e35671, https://doi.org/10.1002/jbm.b.35671.
Due to an oversight in our translation, the first affiliation was incorrectly rendered as “Hunan Normal University Hunan Provincial People's Hospital”; the correct and official English name is “The First-Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital).” Both designations refer to the same institution. The online version of this article has been corrected accordingly.
{"title":"Correction to “Advances in Magnesium Metal and Its Alloys for Promoting Angiogenesis”","authors":"","doi":"10.1002/jbmb.70001","DOIUrl":"10.1002/jbmb.70001","url":null,"abstract":"<p>J. Huang, J. Wu, D. Liu, and P. Gao, “Advances in Magnesium Metal and Its Alloys for Promoting Angiogenesis,” <i>Journal of Biomedical Materials Research Part B: Applied Biomaterials</i> 113, no. 11 (2025): e35671, https://doi.org/10.1002/jbm.b.35671.</p><p>Due to an oversight in our translation, the first affiliation was incorrectly rendered as “Hunan Normal University Hunan Provincial People's Hospital”; the correct and official English name is “The First-Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital).” Both designations refer to the same institution. The online version of this article has been corrected accordingly.</p><p>We apologize for this error.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbmb.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jakari C L Harris, Adam S Verga, Scott J Hollister
3D printing has proven very beneficial in the medical field, especially in cardiovascular medicine, by providing cardiologists and cardiac surgeons with 3D models of patient anatomy for procedure planning. These models are helpful in providing visual aid but are typically simple in design and it is often unknown how the printed model's mechanical properties compare to cardiovascular tissue mechanical properties. Stratasys has developed tissue-mimicking materials; although there are almost no published characterization details on how these printed polyjet materials compare to published human cardiovascular tissue data. We compared J750 digital anatomy printer material properties to left human heart tissue properties using nonlinear elastic constitutive models. We mechanically tested 73 polyjet printer materials (Stratasys J750 DAP) and successfully created a database identifying which printer materials were the best "fit" for each cardiac region of interest. We found that all J750 DAP materials exhibited stiffer behavior than each cardiac tissue region. The top three materials that fit each cardiac region the best were SoftDM400, SolidInternalOrgans_FiberContraction6, and Liver_HighlyContractile, respectively.
{"title":"Matching Polyjet 3D Printed Material Properties to Human Left Heart Tissue Mechanical Behavior.","authors":"Jakari C L Harris, Adam S Verga, Scott J Hollister","doi":"10.1002/jbmb.70002","DOIUrl":"https://doi.org/10.1002/jbmb.70002","url":null,"abstract":"<p><p>3D printing has proven very beneficial in the medical field, especially in cardiovascular medicine, by providing cardiologists and cardiac surgeons with 3D models of patient anatomy for procedure planning. These models are helpful in providing visual aid but are typically simple in design and it is often unknown how the printed model's mechanical properties compare to cardiovascular tissue mechanical properties. Stratasys has developed tissue-mimicking materials; although there are almost no published characterization details on how these printed polyjet materials compare to published human cardiovascular tissue data. We compared J750 digital anatomy printer material properties to left human heart tissue properties using nonlinear elastic constitutive models. We mechanically tested 73 polyjet printer materials (Stratasys J750 DAP) and successfully created a database identifying which printer materials were the best \"fit\" for each cardiac region of interest. We found that all J750 DAP materials exhibited stiffer behavior than each cardiac tissue region. The top three materials that fit each cardiac region the best were SoftDM400, SolidInternalOrgans_FiberContraction6, and Liver_HighlyContractile, respectively.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":"e70002"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oral diseases such as dental caries, periodontitis, and oral cancer remain significant global health challenges due to their high prevalence and potential for severe complications. Conventional diagnostic and therapeutic methods face limitations including insufficient tissue penetration, low spatial resolution, radiation risks, and the emergence of antibiotic resistance. Near-infrared II (NIR-II, 1000-1700 nm) theranostic agents offer deep tissue penetration, high-resolution imaging, and low tissue autofluorescence, enabling precise diagnosis, and targeted treatment integration. This review summarizes the design and development of various NIR-II platforms, including fluorescent probes, photothermal and photodynamic agents, and multifunctional nanomaterials. Key applications in dentistry include early caries detection, guidance for root canal therapy, monitoring of periodontal inflammation, dental implant evaluation, and oral cancer diagnosis and therapy. NIR-II agents enhance antimicrobial efficacy via photothermal and photodynamic effects, promote tissue regeneration, and reduce systemic side effects. Despite promising advances, challenges such as biosafety concerns, limited retention in the dynamic oral environment, and difficulties in imaging mineralized dental tissues remain. Strategies including biodegradable and biomimetic materials, stimuli-responsive delivery systems, and standardized clinical protocols are critical to overcoming these barriers. Looking forward, interdisciplinary collaboration and technological innovation are expected to accelerate clinical translation of NIR-II theranostics, driving a paradigm shift toward precision, minimally invasive dentistry. This emerging approach holds great potential to improve oral health outcomes by enabling safer, more effective, and personalized dental care.
{"title":"Applications of Second Near-Infrared (NIR-II) Theranostic Agents in Dentistry.","authors":"Yiru Liu, Yongzhi Li, Yu Tian","doi":"10.1002/jbmb.70004","DOIUrl":"https://doi.org/10.1002/jbmb.70004","url":null,"abstract":"<p><p>Oral diseases such as dental caries, periodontitis, and oral cancer remain significant global health challenges due to their high prevalence and potential for severe complications. Conventional diagnostic and therapeutic methods face limitations including insufficient tissue penetration, low spatial resolution, radiation risks, and the emergence of antibiotic resistance. Near-infrared II (NIR-II, 1000-1700 nm) theranostic agents offer deep tissue penetration, high-resolution imaging, and low tissue autofluorescence, enabling precise diagnosis, and targeted treatment integration. This review summarizes the design and development of various NIR-II platforms, including fluorescent probes, photothermal and photodynamic agents, and multifunctional nanomaterials. Key applications in dentistry include early caries detection, guidance for root canal therapy, monitoring of periodontal inflammation, dental implant evaluation, and oral cancer diagnosis and therapy. NIR-II agents enhance antimicrobial efficacy via photothermal and photodynamic effects, promote tissue regeneration, and reduce systemic side effects. Despite promising advances, challenges such as biosafety concerns, limited retention in the dynamic oral environment, and difficulties in imaging mineralized dental tissues remain. Strategies including biodegradable and biomimetic materials, stimuli-responsive delivery systems, and standardized clinical protocols are critical to overcoming these barriers. Looking forward, interdisciplinary collaboration and technological innovation are expected to accelerate clinical translation of NIR-II theranostics, driving a paradigm shift toward precision, minimally invasive dentistry. This emerging approach holds great potential to improve oral health outcomes by enabling safer, more effective, and personalized dental care.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":"e70004"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tugce Yesilyurt, Sumeyye Cesur, Onur Alptürk, Elif Damla Arısan, Pınar Obakan, Savas Evran, Oguzhan Gunduz, Esma Özerol, Roger Narayan, Cem Bulent Ustundag
In this work, we generate Janus-type microbubbles that have distinct morphological patterns by utilizing microfluidics technology. A V-Junction Microfluidic (VJM) device was designed through a Solidworks program with three inlets and one microfluidic outlet attached at 30° to produce a closed system. 3% wt. PLA and 3% wt. PEG were chosen owing to the distinct physicochemical features of these polymers. Two hundred and seventy-five microliters/min PLA and 180 μL/min PEG were fed into the system with a nitrogen gas pressure of 12 kPa, where five different solvents produced Janus-type microbubbles. In addition, the effect of the change in inlet velocity of nitrogen gas on the composition of the solutions, volume fraction, and density changes was numerically examined. The results indicate that honeycomb structures and particle formation were observed at different scales, ranging from 854 ± 49 nm to 6.5 ± 0.5 μm. Numerical analysis showed that the speed associated with the 3 wt.% PLA and 3 wt.% PEG solutions had a direct effect on phase formation. Numerical results also showed that the difference in the inlet velocity of nitrogen gas in the apparatus can play a significant role in the composition of the solutions; this change may also affect the formation of microbubbles.
{"title":"Nanosize Polymeric Foams and Microparticles Prepared In Situ From Janus-Type Microbubble Constitutions","authors":"Tugce Yesilyurt, Sumeyye Cesur, Onur Alptürk, Elif Damla Arısan, Pınar Obakan, Savas Evran, Oguzhan Gunduz, Esma Özerol, Roger Narayan, Cem Bulent Ustundag","doi":"10.1002/jbmb.70006","DOIUrl":"https://doi.org/10.1002/jbmb.70006","url":null,"abstract":"<p>In this work, we generate Janus-type microbubbles that have distinct morphological patterns by utilizing microfluidics technology. A V-Junction Microfluidic (VJM) device was designed through a Solidworks program with three inlets and one microfluidic outlet attached at 30° to produce a closed system. 3% wt. PLA and 3% wt. PEG were chosen owing to the distinct physicochemical features of these polymers. Two hundred and seventy-five microliters/min PLA and 180 μL/min PEG were fed into the system with a nitrogen gas pressure of 12 kPa, where five different solvents produced Janus-type microbubbles. In addition, the effect of the change in inlet velocity of nitrogen gas on the composition of the solutions, volume fraction, and density changes was numerically examined. The results indicate that honeycomb structures and particle formation were observed at different scales, ranging from 854 ± 49 nm to 6.5 ± 0.5 μm. Numerical analysis showed that the speed associated with the 3 wt.% PLA and 3 wt.% PEG solutions had a direct effect on phase formation. Numerical results also showed that the difference in the inlet velocity of nitrogen gas in the apparatus can play a significant role in the composition of the solutions; this change may also affect the formation of microbubbles.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbmb.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luise Schlotterose, Duygu Dengiz, Meryem G. Ersahin, Eckhard Quandt, Karsten Steffens, Dennis Schade, Francois Cossais, Ralph Lucius, Kirsten Hattermann
Glial scarring creates a significant obstacle for axonal regeneration in the central nervous system after injury and represents one of the main hurdles for neural microelectrode development. In this study, we established a test system for evaluating potential therapeutics and biomaterials prior to in vivo studies. The human cell line-based in vitro model replicates key glial scar characteristics such as galectin-3 expression and extracellular matrix accumulation. Moreover, we demonstrated how the model can be used to assess and validate new drug targets to reduce glial scar formation by modulating the transforming growth factor-β receptor types I and II. Beyond drug testing, our approach integrates a broad biomaterials science perspective, combining innovative chemical fabrication techniques with a complex in vitro co-stimulation system to investigate biological responses at the cell-material interface. To exemplify this, we explored the effects of sputter-coated free-standing nitinol as an exemplary implant material, along with gold and platinum electrode surfaces with varying characteristics, on glial scar-associated gene expression. By leveraging bioinspired material strategies, this platform enables the validation of promising drug candidates and their modes of action while optimizing neural implant materials to limit glial scar formation. Ultimately, this approach accelerates the development of strategies for central nervous system regeneration.
{"title":"Targeting the Glial Scar: Biomaterial and Drug-Based Strategies for Modulation In Vitro","authors":"Luise Schlotterose, Duygu Dengiz, Meryem G. Ersahin, Eckhard Quandt, Karsten Steffens, Dennis Schade, Francois Cossais, Ralph Lucius, Kirsten Hattermann","doi":"10.1002/jbmb.70007","DOIUrl":"https://doi.org/10.1002/jbmb.70007","url":null,"abstract":"<p>Glial scarring creates a significant obstacle for axonal regeneration in the central nervous system after injury and represents one of the main hurdles for neural microelectrode development. In this study, we established a test system for evaluating potential therapeutics and biomaterials prior to in vivo studies. The human cell line-based in vitro model replicates key glial scar characteristics such as galectin-3 expression and extracellular matrix accumulation. Moreover, we demonstrated how the model can be used to assess and validate new drug targets to reduce glial scar formation by modulating the transforming growth factor-β receptor types I and II. Beyond drug testing, our approach integrates a broad biomaterials science perspective, combining innovative chemical fabrication techniques with a complex in vitro co-stimulation system to investigate biological responses at the cell-material interface. To exemplify this, we explored the effects of sputter-coated free-standing nitinol as an exemplary implant material, along with gold and platinum electrode surfaces with varying characteristics, on glial scar-associated gene expression. By leveraging bioinspired material strategies, this platform enables the validation of promising drug candidates and their modes of action while optimizing neural implant materials to limit glial scar formation. Ultimately, this approach accelerates the development of strategies for central nervous system regeneration.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbmb.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thirumalai Deepak, Nagarajan Janani, Surendran Vivek, Sarah Biju, Lakshminath Kundanati
An ideal meniscus substitute should possess a remarkable resemblance to the native meniscus, which includes structural integrity, biocompatibility, mechanical strength, and durability. Understanding the meniscus anatomy, microarchitecture, and biomechanical properties of the meniscus can help in developing innovative designs, and advancements for addressing meniscus injuries and replacement. Emphasis is made on the promising application of decellularized extracellular matrix (dECM) as a source material for bioink in meniscus 3D printing. This paper examines the criteria required for dECM bioink, including immunogenicity, ECM composition, printability, biocompatibility, biomechanical properties, types of cross-linkers, and their potential applications. By comprehensively addressing these factors, researchers can optimize dECM bioink for 3D printing and develop meniscus substitutes that closely mimic the native meniscus. In conclusion, this review highlights the most promising approaches for 3D printing the meniscus, drawing on the insights gained from the analysis of meniscus anatomy, biomechanics, and dECM bioink characteristics.
{"title":"A Review on Decellularized Extracellular Matrix-Based 3D Printing for Meniscus Regeneration","authors":"Thirumalai Deepak, Nagarajan Janani, Surendran Vivek, Sarah Biju, Lakshminath Kundanati","doi":"10.1002/jbmb.70003","DOIUrl":"https://doi.org/10.1002/jbmb.70003","url":null,"abstract":"<div>\u0000 \u0000 <p>An ideal meniscus substitute should possess a remarkable resemblance to the native meniscus, which includes structural integrity, biocompatibility, mechanical strength, and durability. Understanding the meniscus anatomy, microarchitecture, and biomechanical properties of the meniscus can help in developing innovative designs, and advancements for addressing meniscus injuries and replacement. Emphasis is made on the promising application of decellularized extracellular matrix (dECM) as a source material for bioink in meniscus 3D printing. This paper examines the criteria required for dECM bioink, including immunogenicity, ECM composition, printability, biocompatibility, biomechanical properties, types of cross-linkers, and their potential applications. By comprehensively addressing these factors, researchers can optimize dECM bioink for 3D printing and develop meniscus substitutes that closely mimic the native meniscus. In conclusion, this review highlights the most promising approaches for 3D printing the meniscus, drawing on the insights gained from the analysis of meniscus anatomy, biomechanics, and dECM bioink characteristics.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145618890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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