Hyaluronidase inhibits TGF-β-mediated rat periodontal ligament fibroblast expression of collagen and myofibroblast markers: An in vitro exploration of periodontal tissue remodeling

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Archives of oral biology Pub Date : 2024-04-26 DOI:10.1016/j.archoralbio.2024.105980
Junlin Li , Chen Chen , Yunting Zeng , Jiaqi Lu , Liwei Xiao
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Abstract

Objective

To determine the effect of hyaluronic acid (HA) degradation by hyaluronidase (HYAL) in inhibiting collagen fiber production by rat periodontal ligament cells (rPDLCs).

Design

Primary rPDLCs were isolated from the euthanized rats and used for in vitro experiments. The appropriate HYAL concentration was determined through CCK-8 testing for cytotoxicity detection and Alizarin red staining for mineralization detection. RT-qPCR and western blot assays were conducted to assess the effect of HYAL, with or without TGF-β, on generation of collagen fiber constituents and expression of actin alpha 2, smooth muscle (ACTA2) of rPDLCs.

Results

Neither cell proliferation nor mineralization were significantly affected by treatment with 4 U/mL HYAL. HYAL (4 U/mL) alone downregulated type I collagen fiber (Col1a1 and Col1a2) and Acta2 mRNA expression; however, ACTA2 and COL1 protein levels were only downregulated by HYAL treatment after TGF-β induction.

Conclusions

Treatment of rPDLCs with HYAL can inhibit TGF-β-induced collagen matrix formation and myofibroblast transformation.

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透明质酸酶抑制 TGF-β 介导的大鼠牙周韧带成纤维细胞表达胶原和肌成纤维细胞标记物:牙周组织重塑的体外探索
目的 确定透明质酸(HA)被透明质酸酶(HYAL)降解对抑制大鼠牙周韧带细胞(rPDLCs)产生胶原纤维的影响。通过 CCK-8 检测细胞毒性和茜素红染色检测矿化度来确定适当的 HYAL 浓度。结果4 U/mL的HYAL处理对细胞增殖和矿化均无明显影响。结论用 HYAL 处理 rPDLCs 可抑制 TGF-β 诱导的胶原基质形成和肌成纤维细胞转化。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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