Exploring the Impact of Simulated Microgravity on Cellular DNA: A Comparative Analysis of Cancer and Normal Cell Lines

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-04-30 DOI:10.1007/s12217-024-10116-w

Saifaldeen Altaie, Amera Alrawi, Xuexin Duan, Qater Alnada

{"title":"Exploring the Impact of Simulated Microgravity on Cellular DNA: A Comparative Analysis of Cancer and Normal Cell Lines","authors":"Saifaldeen Altaie, Amera Alrawi, Xuexin Duan, Qater Alnada","doi":"10.1007/s12217-024-10116-w","DOIUrl":null,"url":null,"abstract":"<div><p>The examination of the impact of microgravity on biological systems has gained considerable attention owing to its potential implications for health and disease. Simulated microgravity serves as a valuable methodology for elucidating the intricate cellular responses to altered gravitational conditions. This study investigates the effects of simulated microgravity on cellular DNA, employing four distinct cell lines—breast, brain, and esophageal cancer cells, in conjunction with normal cells for comparative analysis. The experiment utilized the comet assay test to quantitatively assess DNA damage. The results revealed a discernible disparity in the response to simulated microgravity, notably with cancer cells exhibiting a significant increase in DNA damage compared to the relatively minimal effects observed in both control and normal cells. Furthermore, within the cancer cell lines, significant variations in the extent of DNA damage were evident, implying a cell type-dependent response to simulated microgravity. These findings illuminate the potential differential susceptibility of cancerous and normal cells to microgravity-induced DNA damage. Consequently, this research substantially contributes to our comprehension of microgravity-induced cellular responses and unveils promising avenues for targeted interventions in cancer therapy.</p></div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s12217-024-10116-w","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

The examination of the impact of microgravity on biological systems has gained considerable attention owing to its potential implications for health and disease. Simulated microgravity serves as a valuable methodology for elucidating the intricate cellular responses to altered gravitational conditions. This study investigates the effects of simulated microgravity on cellular DNA, employing four distinct cell lines—breast, brain, and esophageal cancer cells, in conjunction with normal cells for comparative analysis. The experiment utilized the comet assay test to quantitatively assess DNA damage. The results revealed a discernible disparity in the response to simulated microgravity, notably with cancer cells exhibiting a significant increase in DNA damage compared to the relatively minimal effects observed in both control and normal cells. Furthermore, within the cancer cell lines, significant variations in the extent of DNA damage were evident, implying a cell type-dependent response to simulated microgravity. These findings illuminate the potential differential susceptibility of cancerous and normal cells to microgravity-induced DNA damage. Consequently, this research substantially contributes to our comprehension of microgravity-induced cellular responses and unveils promising avenues for targeted interventions in cancer therapy.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

探索模拟微重力对细胞 DNA 的影响：癌症细胞系与正常细胞系的比较分析

由于微重力对健康和疾病的潜在影响，研究微重力对生物系统的影响已经获得了相当多的关注。模拟微重力是阐明细胞对改变的重力条件的复杂反应的重要方法。本研究采用四种不同的细胞系--乳腺癌、脑癌和食管癌细胞，以及正常细胞进行比较分析，研究模拟微重力对细胞DNA的影响。实验利用彗星试验来定量评估DNA损伤。结果显示，癌细胞对模拟微重力的反应存在明显差异，特别是与对照细胞和正常细胞中观察到的相对最小的影响相比，癌细胞的DNA损伤显著增加。此外，在癌细胞系中，DNA损伤程度明显不同，这意味着细胞类型对模拟微重力的反应具有依赖性。这些发现揭示了癌细胞和正常细胞对微重力诱导的DNA损伤的潜在易感性差异。因此，这项研究大大有助于我们理解微重力诱导的细胞反应，并为癌症治疗的靶向干预开辟了前景广阔的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.