Pascal Eusemann, Jana Rees, Vivian Kuhlenkamp, Paul Lippitsch, Heiner Schumann
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引用次数: 0
Abstract
Metabarcoding is emerging as an alternative to morphological methods in noninvasive carnivore diet analysis based on scats. A number of metabarcoding markers have been developed but their comparative performance to recover DNA from scats remains mostly untested. We tested three markers covering a wide taxonomic range of prey items and compared them with the results of a morphological analysis. Morphological and genetic methods performed comparably regarding the identity of detected prey species, but the number of identified species varied strongly between markers. Only one, 12S-V5, amplified successfully in all samples and proved to be robust and reliable when working with the highly degraded DNA obtained from scats.
在基于粪便的非侵入性食肉动物饮食分析中，元条码正在成为形态学方法的替代方法。目前已开发出许多元条码标记，但它们从粪便中回收 DNA 的比较性能大多仍未经过测试。我们测试了涵盖猎物分类范围广泛的三种标记物，并将它们与形态分析结果进行了比较。形态学方法和遗传学方法在检测到的猎物物种识别方面表现相当，但不同标记的识别物种数量差异很大。只有一种标记（12S-V5）能在所有样本中成功扩增，并且在处理从粪便中获得的高度降解的 DNA 时证明是稳健可靠的。
期刊介绍：
Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.
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