Intra-peritoneal lavage of Zingiber officinale rhizome and its active constituent gingerol impede inflammation, angiogenesis, and fibrosis following post-operative peritoneal adhesion in male rats

IF 3 3区 医学 Q2 PHARMACOLOGY & PHARMACY Saudi Pharmaceutical Journal Pub Date : 2024-04-30 DOI:10.1016/j.jsps.2024.102092
Roghayeh Yahyazadeh , Vafa Baradaran Rahimi , Seyed Ahmad Mohajeri , Milad Iranshahy , Maede Hasanpour , Vahid Reza Askari
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Abstract

Post-operative peritoneal adhesions (PA) are a common and important clinical problem. In this study, we focused on the ameliorative efficacy of ginger and gingerol compounds on surgical-induced peritoneal adhesion, and their strategies that disrupted the PA formation pathways to suppress their incidence. First, liquid chromatography-mass spectrometry (LC-MS) was established to separate and identify several chemical groups of ginger rhizome extract. In the next steps, male Wistar albino rats were randomly selected and divided into various groups, namely sham, control, ginger extract (0.6, 1.8, 5 %w/v), and gingerol (0.05, 0.1, 0.3, and 1 %w/v). Finally, we investigated the macroscopic parameters such as wound healing, body weight as well as spleen height and weight. In addition, visual peritoneal adhesion assessment was performed via Nair et al and Adhesion Scoring Scheme. Moreover, the microscopic parameters and biological assessment was performed via and immunoassays. The present findings revealed significant improvement in wound healing and reduction of the adhesion range, as Nair et al. and Adhesion Scoring Scheme scoring, in both the ginger and gingerol groups compared to the PA group (P < 0.05). Whereas, gingerol (0.3 % w/v) was able to increase the body weight in rats (P < 0.0001) at end stage of experiment. Also, inflammation, angiogenesis, and fibrosis were significantly decreased due to the downregulation of interleukin (IL)-6, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, vascular endothelial growth factor (VEGF), respectively, in the ginger and gingerol groups compared to the PA group (P < 0.05). In contrast, the levels of IL-10 were increased in the ginger and gingerol groups compared to the control group (P < 0.01). Our results proved that ginger rhizome and gingerol, as novel therapeutic compounds, could be used to prevent PA for their beneficial anti-inflammatory as well as anti-fibrosis properties in clinical trials. However, further clinical studies are required to approve the effectiveness of ginger and gingerol.

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雄性大鼠术后腹膜粘连后腹腔内灌洗姜科植物姜的根茎及其活性成分姜酚能抑制炎症、血管生成和纤维化
术后腹膜粘连(PA)是一个常见且重要的临床问题。在这项研究中,我们重点研究了生姜和姜酚化合物对手术引起的腹膜粘连的改善作用,以及它们破坏 PA 形成途径以抑制其发生的策略。首先,通过液相色谱-质谱联用技术(LC-MS)分离鉴定了生姜根茎提取物中的多个化学组。随后,随机选取雄性 Wistar 白化大鼠,将其分为假组、对照组、生姜提取物组(0.6%、1.8%、5%w/v)和姜酚组(0.05%、0.1%、0.3%、1%w/v)。最后,我们研究了伤口愈合、体重、脾脏高度和重量等宏观参数。此外,我们还通过 Nair 等人和粘附评分计划对腹膜粘附进行了目测评估。此外,还通过免疫测定进行了显微参数和生物评估。本研究结果显示,与 PA 组相比(P < 0.05),生姜组和姜酚组的伤口愈合情况均有明显改善,粘附范围也有所减少(Nair et al.而姜酚(0.3 % w/v)在实验结束阶段能够增加大鼠的体重(P < 0.0001)。此外,与 PA 组相比,生姜组和姜酚组的白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α、转化生长因子(TGF)-β1、血管内皮生长因子(VEGF)分别下调,因此炎症、血管生成和纤维化明显减轻(P < 0.05)。相反,与对照组相比,生姜组和姜辣素组的 IL-10 水平升高(P < 0.01)。我们的研究结果证明,生姜根茎和姜辣素作为新型治疗化合物,具有抗炎和抗纤维化的功效,可用于临床试验预防 PA。然而,生姜和姜酚的有效性还需要进一步的临床研究来验证。
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来源期刊
Saudi Pharmaceutical Journal
Saudi Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
6.10
自引率
2.40%
发文量
194
审稿时长
67 days
期刊介绍: The Saudi Pharmaceutical Journal (SPJ) is the official journal of the Saudi Pharmaceutical Society (SPS) publishing high quality clinically oriented submissions which encompass the various disciplines of pharmaceutical sciences and related subjects. SPJ publishes 8 issues per year by the Saudi Pharmaceutical Society, with the cooperation of the College of Pharmacy, King Saud University.
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