Jiewen Guan, Alison Lacombe, Bhargavi Rane, Jared Van Blair, Yujie Zhang, Juming Tang, Shyam Sablani, Vivian C. H. Wu
{"title":"The stress response of Listeria monocytogenes inoculated on fresh apples exposed to gaseous chlorine dioxide","authors":"Jiewen Guan, Alison Lacombe, Bhargavi Rane, Jared Van Blair, Yujie Zhang, Juming Tang, Shyam Sablani, Vivian C. H. Wu","doi":"10.1111/jfs.13126","DOIUrl":null,"url":null,"abstract":"<p>Fresh apples are vulnerable to Listeria monocytogenes (L. monocytogenes) contamination during handling and storage causing subsequent foodborne outbreaks. Gaseous chlorine dioxide (ClO<sub>2</sub>) is an effective antimicrobial agent against L. monocytogenes and could augment food safety. This study investigates the stress and virulence response of L. monocytogenes on apples exposed to a sublethal concentration of ClO<sub>2</sub>. L. monocytogenes was inoculated on the surface of apples and exposed to gaseous ClO<sub>2</sub> for 1 h. Gene expression analysis via Real-Time quantitative polymerase chain reaction (RT-qPCR) revealed minimal changes in gene expression compared to control samples. However, a surface-dependent response was observed, with slight upregulation of relevant virulence (hly) and stress (clpC) genes on more hydrophilic surfaces. These findings indicate that gaseous ClO<sub>2</sub> treatment can provide a biostatic effect against L. monocytogenes on fresh produce, shedding light on its antimicrobial mechanism and potential for enhancing apple safety.</p>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"44 3","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfs.13126","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Safety","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jfs.13126","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Fresh apples are vulnerable to Listeria monocytogenes (L. monocytogenes) contamination during handling and storage causing subsequent foodborne outbreaks. Gaseous chlorine dioxide (ClO2) is an effective antimicrobial agent against L. monocytogenes and could augment food safety. This study investigates the stress and virulence response of L. monocytogenes on apples exposed to a sublethal concentration of ClO2. L. monocytogenes was inoculated on the surface of apples and exposed to gaseous ClO2 for 1 h. Gene expression analysis via Real-Time quantitative polymerase chain reaction (RT-qPCR) revealed minimal changes in gene expression compared to control samples. However, a surface-dependent response was observed, with slight upregulation of relevant virulence (hly) and stress (clpC) genes on more hydrophilic surfaces. These findings indicate that gaseous ClO2 treatment can provide a biostatic effect against L. monocytogenes on fresh produce, shedding light on its antimicrobial mechanism and potential for enhancing apple safety.
期刊介绍：
The Journal of Food Safety emphasizes mechanistic studies involving inhibition, injury, and metabolism of food poisoning microorganisms, as well as the regulation of growth and toxin production in both model systems and complex food substrates. It also focuses on pathogens which cause food-borne illness, helping readers understand the factors affecting the initial detection of parasites, their development, transmission, and methods of control and destruction.
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