Inflammation and tissue remodeling mediator expression during gingivitis: A comparison between experimental, naturally occurring gingivitis, and periodontal health

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontology Pub Date : 2024-05-06 DOI:10.1002/jper.23-0692
Yung‐Ting Hsu, Ana M. Chang, Diane Daubert, Frank Roberts, Dandan Chen, Harsh M. Trivedi, Juliana Gomez, Rich P. Darveau
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Abstract

BackgroundThe aim of this study is to evaluate the immune regulation and tissue remodeling responses during experimental gingivitis (EG) and naturally occurring gingivitis (NG) to provide a comprehensive analysis of host responses. Gingival crevicular fluid (GCF) was obtained from 2 human studies conducted in university settings.MethodsThe EG study enrolling 26 volunteers provided controls for the baseline (Day 0) from healthy disease‐free participants, while Day 21 (the end of EG induction of the same group) was used to represent EG. Twenty‐six NG participants age‐matched with those of the EG group were recruited. GCF samples were analyzed for 39 mediators of inflammatory/immune responses and tissue remodeling using commercially available bead‐based multiplex immunoassays. The differences in GI and mediator expression among groups were determined at a 95% confidence level (p ≤ 0.05) by a 2‐way analysis of variance (ANOVA) with a post‐hoc Tukey's test.ResultsOur findings showed that EG had a greater gingival index than NG and was healthy (p < 0.01 of all comparisons). Furthermore, EG showed significantly higher levels of MPO (p < 0.001), CCL3 (p < 0.05), and IL‐1B (p < 0.001) than NG. In contrast, NG had increased levels of MIF (p < 0.05), Fractalkine (p < 0.001), angiogenin (p < 0.05), C3a (p < 0.001), BMP‐2 (p < 0.001), OPN (p < 0.05), RANKL (p < 0.001), and MMP‐13 (p < 0.001) than EG.ConclusionsConsistent with the findings from chronic (NG) versus acute (EG) inflammatory lesions, these data reveal that NG displays greater immune regulation, angiogenesis, and bone remodeling compared to EG.
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牙龈炎期间炎症和组织重塑介质的表达:实验性牙龈炎、自然牙龈炎与牙周健康之间的比较
背景本研究旨在评估实验性牙龈炎(EG)和自然发生的牙龈炎(NG)期间的免疫调节和组织重塑反应,从而对宿主反应进行全面分析。方法EG研究为26名志愿者提供了基线(第0天)对照,来自健康无病的参与者,而第21天(同组参与者EG诱导结束时)则代表EG。另外还招募了 26 名与 EG 组年龄相匹配的 NG 参与者。使用市售的基于微珠的多重免疫测定法对 GCF 样品中的 39 种炎症/免疫反应和组织重塑介质进行了分析。结果我们的研究结果表明,与 NG 相比,EG 的牙龈指数更高,而且更健康(所有比较的 p 均为 0.01)。此外,EG 的 MPO(p < 0.001)、CCL3(p < 0.05)和 IL-1B (p < 0.001)水平明显高于 NG。相比之下,NG 的 MIF(p <;0.05)、Fractalkine(p <;0.001)、血管生成素(p <;0.05)、C3a(p <;0.001)、BMP-2(p <;0.001)、OPN(p <;0.05)、RANKL(p <;0.001)和 MMP-13 (p <;0.结论与慢性(NG)与急性(EG)炎性病变的研究结果一致,这些数据显示,与 EG 相比,NG 显示出更强的免疫调节、血管生成和骨重塑能力。
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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