A bamboo ‘PeSAPK4-PeMYB99-PeTIP4-3’ regulatory model involved in water transport

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-05-06 DOI:10.1111/nph.19787

Chenglei Zhu, Zeming Lin, Kebin Yang, Yongfeng Lou, Yan Liu, Tiankuo Li, Hui Li, Xiaolin Di, Jiangfei Wang, Huayu Sun, Ying Li, Xueping Li, Zhimin Gao

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Abstract

Water plays crucial roles in expeditious growth and osmotic stress of bamboo. Nevertheless, the molecular mechanism of water transport remains unclear.
In this study, an aquaporin gene, PeTIP4-3, was identified through a joint analysis of root pressure and transcriptomic data in moso bamboo (Phyllostachys edulis). PeTIP4-3 was highly expressed in shoots, especially in the vascular bundle sheath cells. Overexpression of PeTIP4-3 could increase drought and salt tolerance in transgenic yeast and rice.
A co-expression pattern of PeSAPK4, PeMYB99 and PeTIP4-3 was revealed by WGCNA. PeMYB99 exhibited an ability to independently bind to and activate PeTIP4-3, which augmented tolerance to drought and salt stress. PeSAPK4 could interact with and phosphorylate PeMYB99 in vivo and in vitro, wherein they synergistically accelerated PeTIP4-3 transcription. Overexpression of PeMYB99 and PeSAPK4 also conferred drought and salt tolerance in transgenic rice.
Further ABA treatment analysis indicated that PeSAPK4 enhanced water transport in response to stress via ABA signaling. Collectively, an ABA-mediated cascade of PeSAPK4-PeMYB99-PeTIP4-3 is proposed, which governs water transport in moso bamboo.

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参与水运输的竹子 "PeSAPK4-PeMYB99-PeTIP4-3 "调控模型

摘要水对竹子的快速生长和渗透胁迫起着至关重要的作用。然而，水分运输的分子机制仍不清楚。本研究通过对毛竹（Phyllostachys edulis）根压和转录组数据的联合分析，发现了一个水蒸发蛋白基因--PeTIP4-3。PeTIP4-3在笋中高表达，尤其是在维管束鞘细胞中。过表达 PeTIP4-3 可提高转基因酵母和水稻的耐旱性和耐盐性。WGCNA揭示了PeSAPK4、PeMYB99和PeTIP4-3的共表达模式。PeMYB99 能够独立地与 PeTIP4-3 结合并激活 PeTIP4-3，从而增强对干旱和盐胁迫的耐受性。PeSAPK4 可在体内和体外与 PeMYB99 相互作用并使其磷酸化，它们协同加速了 PeTIP4-3 的转录。过表达 PeMYB99 和 PeSAPK4 还能赋予转基因水稻抗旱和抗盐能力。进一步的 ABA 处理分析表明，PeSAPK4 通过 ABA 信号转导增强了水运输对胁迫的响应。综上所述，提出了一个由 ABA 介导的 PeSAPK4-PeMYB99-PeTIP4-3 级联，它控制着毛竹的水分运输。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.