Oral administration of Lactobacillus delbrueckii subsp. lactis LDL557 attenuates airway inflammation and changes the gut microbiota in a Der p-sensitized mouse model of allergic asthma.

IF 2.3 4区 医学 Q3 ALLERGY Asian Pacific journal of allergy and immunology Pub Date : 2024-05-06 DOI:10.12932/AP-200823-1672
Pei-Chi Chen, Han-Yin Hsu, Yi-Chu Liao, Chia-Chia Lee, Miao-Hsi Hsieh, Wen-Shou Kuo, Lawrence Shih-Hsin Wu, Jiu-Yao Wang
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Abstract

Background: Lactic acid bacteria may be used as probiotics to prevent or treat various diseases, and Lactobacillus delbrueckii has an inhibitory effect on the development of atopic diseases.

Objective: This study explored the effects of L. delbrueckii subsp. lactis strain LDL557 administration on a mouse asthma model resulting from Dermatophoides pteronyssinus (Der p) sensitization and investigated the associated gut microbiota.

Methods: Der p-sensitized and challenged BALB/c mice were orally administered with three different doses of live (low, 10⁷ colony-forming units (CFU); medium, 10⁸ CFU; high, 10⁹ CFU) and heat-killed (10⁹ cells) LDL557 in 200 μL of PBS daily, starting 2 weeks before Der p sensitization and lasting 4 weeks. After the allergen challenge, airway responsiveness to methacholine and the influx of inflammatory cells to the lungs were assessed. The gut microbiome was obtained by sequencing the V3-V4 region of the 16S rRNA gene from mice stool samples.

Results: LDL557 in the live (10⁹ CFU) and heat-killed (10⁹ cells) conditions reduced the airway hyper-responsiveness after stimulation with methacholine, inflammatory cell infiltration, and mucus production. These effects were similar to those in groups treated with dexamethasone. No significant change in the gut microbiota was observed after LDL557 treatment, except for the tendency of heat-killed LDL557 to change the gut microbial profile to a greater extent than live LDL557.

Conclusion: In summary, we found that live and heat-killed LDL557 had the beneficial effect of preventing Der p-induced allergic inflammation in a mouse model of asthma.

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口服德尔布鲁贝克乳杆菌亚种 LDL557 可减轻气道炎症,并改变 Der p 致敏过敏性哮喘小鼠模型的肠道微生物群。
背景:特应性皮炎(AD)是一种慢性炎症性皮肤病,在发达国家多达 20% 的儿童受到影响。虽然益生菌有望成为特应性皮炎的辅助治疗药物,但其作用机制尚不十分清楚:在先前研究的基础上,我们研究了加塞乳杆菌及其月光甘油醛-3-磷酸脱氢酶(GAPDH)(即 LGp40)是否有益于 AD 的治疗:方法:根据 "由外而内 "和 "由内而外 "的假说,我们在使用卵清蛋白(OVA)和Dermatophagoides pteronyssinus(Der p)过敏原的AD小鼠模型(SKH和C57BL/6J小鼠)中,口服L. gasseri和腹腔注射LGp40,研究它们的保护作用。评估包括测量生理、病理和免疫功能参数。为了深入探讨 LGp40 对注意力缺失症的保护作用的详细机制,我们还使用人类皮肤角质细胞(HaCaT)和单核细胞(THP1)细胞系进行了其他试验:结果:L. gasseri 和 LGp40 增强了皮肤屏障功能,提高了皮肤保湿能力。它们还能减少真皮中朗格汉斯细胞的浸润,减轻偏斜的 Th2 和 Th17 免疫反应。此外,LGp40 还能通过阻断 caspase-3 级联抑制过敏原诱导的角质细胞凋亡,并减少巨噬细胞中的 NLR 家族含吡啶域 3(NLRP3)炎性体。这些抑制作用是通过激活过氧化物酶体增殖激活受体γ(PPARγ)途径实现的:本研究的结果提供了一个新的视角,让人们了解益生菌通过月光 GAPDH 蛋白预防和治疗过敏性疾病的作用机制。
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来源期刊
CiteScore
12.80
自引率
0.00%
发文量
74
审稿时长
>12 weeks
期刊介绍: The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.
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