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Effect of budesonide nasal irrigation on Hypothalamic-Pituitary-Adrenal Axis in patients with chronic rhinosinusitis post endoscopic sinus surgery: A prospective study.
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-21 DOI: 10.12932/AP-121124-1968
Jesada Kanjanaumporn, Sorranut Thaweboon, Songklot Aeumjaturapat, Kornkiat Snidvongs, Supinda Chusakul, Kachorn Seresirikachorn, Lalita Prathanee

Background: Corticosteroids added to high volume saline nasal irrigation have been introduced as a more effective method of delivering corticosteroids to the sinuses than nasal sprays. However, information regarding the effect of this intervention on the hypothalamic-pituitary-adrenal (HPA) axis is still limited.

Objective: To evaluate the safety of long-term corticosteroid (6 months) nasal irrigation in patients with chronic rhinosinusitis (CRS) post endoscopic sinus surgery.

Methods: Seventeen patients with CRS were included. After undergoing endoscopic sinus surgery, the patients were prescribed budesonide nasal irrigations (250 ml via squeeze bottle) twice daily (1 mg/day) for six months. The serum morning cortisol levels of these patients were then evaluated at 3 and 6 months post-operatively.

Results: Median serum morning cortisol levels were 10.5 mcg% at pre-operative baseline; 10.3 mcg% at 3 months; and 11.2 mcg% at 6 months on post-operative follow-up. There were no significant changes in the serum morning cortisol levels (P value = 0.71 and 0.63 respectively). Three of 17 patients (17.65%) had mildly abnormal serum morning cortisol levels (4, 4.3 and 4.9 mcg%) at 3 months. However, these levels were within a normal range at 6 months.

Conclusions: Serum morning cortisol levels were not significantly changed after usage of budesonide nasal irrigation for 6 months.

{"title":"Effect of budesonide nasal irrigation on Hypothalamic-Pituitary-Adrenal Axis in patients with chronic rhinosinusitis post endoscopic sinus surgery: A prospective study.","authors":"Jesada Kanjanaumporn, Sorranut Thaweboon, Songklot Aeumjaturapat, Kornkiat Snidvongs, Supinda Chusakul, Kachorn Seresirikachorn, Lalita Prathanee","doi":"10.12932/AP-121124-1968","DOIUrl":"https://doi.org/10.12932/AP-121124-1968","url":null,"abstract":"<p><strong>Background: </strong>Corticosteroids added to high volume saline nasal irrigation have been introduced as a more effective method of delivering corticosteroids to the sinuses than nasal sprays. However, information regarding the effect of this intervention on the hypothalamic-pituitary-adrenal (HPA) axis is still limited.</p><p><strong>Objective: </strong>To evaluate the safety of long-term corticosteroid (6 months) nasal irrigation in patients with chronic rhinosinusitis (CRS) post endoscopic sinus surgery.</p><p><strong>Methods: </strong>Seventeen patients with CRS were included. After undergoing endoscopic sinus surgery, the patients were prescribed budesonide nasal irrigations (250 ml via squeeze bottle) twice daily (1 mg/day) for six months. The serum morning cortisol levels of these patients were then evaluated at 3 and 6 months post-operatively.</p><p><strong>Results: </strong>Median serum morning cortisol levels were 10.5 mcg% at pre-operative baseline; 10.3 mcg% at 3 months; and 11.2 mcg% at 6 months on post-operative follow-up. There were no significant changes in the serum morning cortisol levels (P value = 0.71 and 0.63 respectively). Three of 17 patients (17.65%) had mildly abnormal serum morning cortisol levels (4, 4.3 and 4.9 mcg%) at 3 months. However, these levels were within a normal range at 6 months.</p><p><strong>Conclusions: </strong>Serum morning cortisol levels were not significantly changed after usage of budesonide nasal irrigation for 6 months.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of pre-existing immunity on humoral and cellular responses to CoronaVac in SARS-CoV-2 variants: A focus on common human Coronaviruses. 原有免疫力对 SARS-CoV-2 变体对 CoronaVac 的体液和细胞反应的影响:聚焦常见人类冠状病毒。
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-21 DOI: 10.12932/AP-201124-1979
Nawamin Pinpathomrat, Bunya Seeyankem, Ratchanon Sophonmanee, Jomkwan Ongarj, Smonrapat Surasombatpattana

Background: The global COVID-19 pandemic, caused by SARS-CoV-2, has highlighted the importance of understanding immune responses elicited by vaccines.

Objective: This study evaluated antibody and T cell responses to the inactivated CoronaVac vaccine, as well as the role of pre-existing immunity to common human coronaviruses (HCoVs) in shaping vaccine-induced immunity.

Methods: We enrolled 64 participants (17 males and 47 females) and measured IgG levels against HCoVs before and after vaccination. T cell responses were analysed by stimulating peripheral blood mononuclear cells (PBMCs) with wild-type, Delta, and Omicron spike peptides.

Results: We found pre-existing antibodies against HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43 were present before vaccination. Notably, a positive correlation was observed between pre-existing antibodies to HCoV-229E and HCoV-HKU1 and anti-RBD IgG levels post-vaccination. Pre-existing CD4+ T cell responses were observed for the wild-type strain before vaccination, with a significant reduction in IFN-γ secretion after Delta re-stimulation and partial restoration after Omicron re-stimulation. IL-4 production by CD4+ T cells was significantly reduced upon re-stimulation with Delta and Omicron compared to wild-type. CD8+ T cells again showed a reduction of IL-4 production after Delta re-stimulation compared to the original strain.

Conclusions: This work demonstrate that CoronaVac induces robust humoral and cellular immune responses, though variant-specific responses vary. Pre-existing immunity to certain HCoVs may influence vaccine-induced antibody responses, underscoring the importance of monitoring immunity to emerging SARS-CoV-2 variants and informing future vaccine design.

{"title":"Impact of pre-existing immunity on humoral and cellular responses to CoronaVac in SARS-CoV-2 variants: A focus on common human Coronaviruses.","authors":"Nawamin Pinpathomrat, Bunya Seeyankem, Ratchanon Sophonmanee, Jomkwan Ongarj, Smonrapat Surasombatpattana","doi":"10.12932/AP-201124-1979","DOIUrl":"https://doi.org/10.12932/AP-201124-1979","url":null,"abstract":"<p><strong>Background: </strong>The global COVID-19 pandemic, caused by SARS-CoV-2, has highlighted the importance of understanding immune responses elicited by vaccines.</p><p><strong>Objective: </strong>This study evaluated antibody and T cell responses to the inactivated CoronaVac vaccine, as well as the role of pre-existing immunity to common human coronaviruses (HCoVs) in shaping vaccine-induced immunity.</p><p><strong>Methods: </strong>We enrolled 64 participants (17 males and 47 females) and measured IgG levels against HCoVs before and after vaccination. T cell responses were analysed by stimulating peripheral blood mononuclear cells (PBMCs) with wild-type, Delta, and Omicron spike peptides.</p><p><strong>Results: </strong>We found pre-existing antibodies against HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43 were present before vaccination. Notably, a positive correlation was observed between pre-existing antibodies to HCoV-229E and HCoV-HKU1 and anti-RBD IgG levels post-vaccination. Pre-existing CD4+ T cell responses were observed for the wild-type strain before vaccination, with a significant reduction in IFN-γ secretion after Delta re-stimulation and partial restoration after Omicron re-stimulation. IL-4 production by CD4+ T cells was significantly reduced upon re-stimulation with Delta and Omicron compared to wild-type. CD8+ T cells again showed a reduction of IL-4 production after Delta re-stimulation compared to the original strain.</p><p><strong>Conclusions: </strong>This work demonstrate that CoronaVac induces robust humoral and cellular immune responses, though variant-specific responses vary. Pre-existing immunity to certain HCoVs may influence vaccine-induced antibody responses, underscoring the importance of monitoring immunity to emerging SARS-CoV-2 variants and informing future vaccine design.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Denatonium benzoate promotes IgE-mediated mast cell degranulation and ovalbumin-induced allergic diarrhea in BALB/c mice.
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-21 DOI: 10.12932/AP-250424-1845
Ju Liu, Huaping Xu, Shaotong Feng, Xiaoyun Shi, Xin Li, Jiexin Zou, Linbo Shi

Background: Denatonium benzoate (DB), one of the most bitter compounds known to man, is used for alcohol denaturation. Some reports have demonstrated that asthmatic symptoms are associated with DB exposure, but the possible links between DB and immunoglobulin E (IgE)-mediated allergy susceptibility have not yet been examined.

Objective: This study investigated the effects of DB on in vitro IgE-mediated mast cell degranulation and food allergy in BALB/c mice.

Methods: IgE-sensitized rat RBL-2H3 cells (a basophilic leukemia mast cell line) and human KU812 cells (a basophilic cell line) and mice with ovalbumin (OVA)-induced allergy were treated with DB. Histamine release, calcium ion (Ca²⁺) influx, phosphorylated spleen tyrosine kinase (p-Lyn) and phosphorylated phospholipase C-γ (p-PLCγ) levels, OVA-specific IgE, anaphylactic symptoms, and the cell-surface expression of the high-affinity IgE receptor α-subunit (FcεRIα) on mast cells were evaluated.

Results: DB increased histamine release, Ca²⁺ mobilization, and p-Lyn and p-PLCγ levels in IgE-mediated activated RBL-2H3 and KU812 cells, and enhanced the cell-surface expression of FcεRIα messenger RNA (mRNA). In mice, DB increased the severity of OVA-induced anaphylactic and diarrheic symptoms, the mucus thickness in the jejunum, histamine and OVA-specific IgE levels, and FcεRIα mRNA in isolated mucosal mast cells.

Conclusions: Our work indicates that DB promotes IgE-mediated mast cell degranulation and OVA-induced allergic diarrhea in BALB/c mice, providing evidence that exposure to DB promotes allergy susceptibility.

{"title":"Denatonium benzoate promotes IgE-mediated mast cell degranulation and ovalbumin-induced allergic diarrhea in BALB/c mice.","authors":"Ju Liu, Huaping Xu, Shaotong Feng, Xiaoyun Shi, Xin Li, Jiexin Zou, Linbo Shi","doi":"10.12932/AP-250424-1845","DOIUrl":"https://doi.org/10.12932/AP-250424-1845","url":null,"abstract":"<p><strong>Background: </strong>Denatonium benzoate (DB), one of the most bitter compounds known to man, is used for alcohol denaturation. Some reports have demonstrated that asthmatic symptoms are associated with DB exposure, but the possible links between DB and immunoglobulin E (IgE)-mediated allergy susceptibility have not yet been examined.</p><p><strong>Objective: </strong>This study investigated the effects of DB on in vitro IgE-mediated mast cell degranulation and food allergy in BALB/c mice.</p><p><strong>Methods: </strong>IgE-sensitized rat RBL-2H3 cells (a basophilic leukemia mast cell line) and human KU812 cells (a basophilic cell line) and mice with ovalbumin (OVA)-induced allergy were treated with DB. Histamine release, calcium ion (Ca²⁺) influx, phosphorylated spleen tyrosine kinase (p-Lyn) and phosphorylated phospholipase C-γ (p-PLCγ) levels, OVA-specific IgE, anaphylactic symptoms, and the cell-surface expression of the high-affinity IgE receptor α-subunit (FcεRIα) on mast cells were evaluated.</p><p><strong>Results: </strong>DB increased histamine release, Ca²⁺ mobilization, and p-Lyn and p-PLCγ levels in IgE-mediated activated RBL-2H3 and KU812 cells, and enhanced the cell-surface expression of FcεRIα messenger RNA (mRNA). In mice, DB increased the severity of OVA-induced anaphylactic and diarrheic symptoms, the mucus thickness in the jejunum, histamine and OVA-specific IgE levels, and FcεRIα mRNA in isolated mucosal mast cells.</p><p><strong>Conclusions: </strong>Our work indicates that DB promotes IgE-mediated mast cell degranulation and OVA-induced allergic diarrhea in BALB/c mice, providing evidence that exposure to DB promotes allergy susceptibility.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of exposure to ambient air volatile organic compounds on the severity of atopic dermatitis and lag-day effect.
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-21 DOI: 10.12932/AP-101124-1967
Hui-Wen Tseng

Background: Previous studies reported positive associations between exposure to air volatile organic compounds (VOCs) and daily visits for atopic dermatitis (AD).

Objective: This population-based study investigated associations between air VOCs exposure and daily hospitals visits for AD, severity subgroup (mild and severe), and lag-day effect in central-southern Taiwan.

Methods: The dependent variable was AD with diagnostic codes (ICD-9-CM 691.8 and ICD-10-CM L20) retrieved from the Taiwan National Health Insurance Research Database from 2008/01/01 to 2018/12/31. Independent variables included one-day 75th-percentile value of each VOC (benzene, ethylbenzene, toluene, m-/p-xylene, o-xylene, 1,3,5-trimethylbenzene, 1,2,4-trimethylbenzene, isopentane, n-pentane, n-hexane, methylcyclohexane, and cyclohexane) and four meteorological conditions from the Taiwan Air Quality Monitoring Network Database. This was a case-crossover design with multivariable conditional logistic regression, and the adjusted odds ratios (AORs) were reported.

Results: Concentrations of the 12 air VOCs significantly positively affected the total number of daily visits for AD (AOR = 1.02~1.69, P < 0.001) and subgroups of mild (AOR = 1.001~1.049, P < 0.001) and severe (AOR = 1.002~1.077, P < 0.001). The effect of air VOCs on the severe AD group was higher than that on the mild group. Values of the six VOCs on the 1st lag day (benzene: AOR = 1.16, 1,3,5-trimethylbenzene: AOR = 1.5, 1,2,4-trimethylbenzene: AOR = 1.13, isopentane: AOR = 1.07, n-pentane: AOR = 1.08, methylcyclohexane: AOR = 1.5, all P < 0.05) were significantly positively associated with the number of daily visits for AD.

Conclusions: Exposure to the 12 air VOCs on the visit days increased the risks of daily visits for AD in total and severity subgroup. The effects of six certain VOCs on the 1st lag day were significant positive.

{"title":"Effect of exposure to ambient air volatile organic compounds on the severity of atopic dermatitis and lag-day effect.","authors":"Hui-Wen Tseng","doi":"10.12932/AP-101124-1967","DOIUrl":"https://doi.org/10.12932/AP-101124-1967","url":null,"abstract":"<p><strong>Background: </strong>Previous studies reported positive associations between exposure to air volatile organic compounds (VOCs) and daily visits for atopic dermatitis (AD).</p><p><strong>Objective: </strong>This population-based study investigated associations between air VOCs exposure and daily hospitals visits for AD, severity subgroup (mild and severe), and lag-day effect in central-southern Taiwan.</p><p><strong>Methods: </strong>The dependent variable was AD with diagnostic codes (ICD-9-CM 691.8 and ICD-10-CM L20) retrieved from the Taiwan National Health Insurance Research Database from 2008/01/01 to 2018/12/31. Independent variables included one-day 75th-percentile value of each VOC (benzene, ethylbenzene, toluene, m-/p-xylene, o-xylene, 1,3,5-trimethylbenzene, 1,2,4-trimethylbenzene, isopentane, n-pentane, n-hexane, methylcyclohexane, and cyclohexane) and four meteorological conditions from the Taiwan Air Quality Monitoring Network Database. This was a case-crossover design with multivariable conditional logistic regression, and the adjusted odds ratios (AORs) were reported.</p><p><strong>Results: </strong>Concentrations of the 12 air VOCs significantly positively affected the total number of daily visits for AD (AOR = 1.02~1.69, P < 0.001) and subgroups of mild (AOR = 1.001~1.049, P < 0.001) and severe (AOR = 1.002~1.077, P < 0.001). The effect of air VOCs on the severe AD group was higher than that on the mild group. Values of the six VOCs on the 1st lag day (benzene: AOR = 1.16, 1,3,5-trimethylbenzene: AOR = 1.5, 1,2,4-trimethylbenzene: AOR = 1.13, isopentane: AOR = 1.07, n-pentane: AOR = 1.08, methylcyclohexane: AOR = 1.5, all P < 0.05) were significantly positively associated with the number of daily visits for AD.</p><p><strong>Conclusions: </strong>Exposure to the 12 air VOCs on the visit days increased the risks of daily visits for AD in total and severity subgroup. The effects of six certain VOCs on the 1st lag day were significant positive.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of daily aspirin intake on platelet-associated factors and aggregation in nonsteroidal anti-inflammatory drug exacerbated respiratory disease: A cross-sectional study.
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-21 DOI: 10.12932/AP-261024-1959
Selcan Genc, Basak Ezgi Sarac, Ozge Can Bostan, Gulseren Tuncay, Hayriye Akel Bilgic, Baran Erman, Umit Sahiner, Gul Karakaya, Ali Fuat Kalyoncu, Ebru Damadoglu, Cagatay Karaaslan

Background: Nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD) is a clinical syndrome characterized by chronic rhinosinusitis with nasal polyposis (CRSwNP), adult-onset asthma and hypersensitivity to NSAIDs. Long-term aspirin treatment after desensitization (ATAD) is used for clinical improvement in N-ERD patients. However, information on the potential effect of ATAD on the platelet-neutrophil aggregates (PNA) level in N-ERD patients is highly limited.

Objective: This study aimed to explore the impact of PNA on the pathogenesis of N-ERD and the potential effect of ATAD on N-ERD patient profiles from a platelet point-of-view.

Methods: Sixty-one individuals were enrolled, including 16 N-ERD patients with ATAD (ATAD+), 15 N-ERD patients without ATAD (ATAD-), 15 aspirin-tolerant asthma (ATA) patients, and 15 healthy controls (HCs). Lipid mediators classical in N-ERD, including urinary-LTE4 (uLTE4), prostaglandin-D2 (PGD2), and prostaglandin-E2 (PGE2) were assessed by ELISA. Platelet activation was estimated based on expression levels of sP-selectin, CD40L, Platelet Factor-4 (PF4), RANTES, Thromboxane-A2 (TXA2), PAF, 12-HETE in plasma levels by ELISA; and PNA percentage by flow cytometry.

Results: ATAD+; 12-HETE, and PF4 levels were remarkably low, while higher levels were determined in ATAD- and ATA groups. ATAD+; uLTE4 levels were positively correlated with 12-HETE. Another positive correlation was detected between sP-selectin and 12-HETE in ATAD-. Compared to HCs, it was found that among all N-ERD patients, significant increase in PNA.

Conclusions: Plasma levels of PGE2, PF4, and 12-HETE appear to be affected by aspirin treatment. We believe that 12-HETE could play a significant role in the N-ERD pathogenesis by contributing to platelet activation.

{"title":"Analysis of daily aspirin intake on platelet-associated factors and aggregation in nonsteroidal anti-inflammatory drug exacerbated respiratory disease: A cross-sectional study.","authors":"Selcan Genc, Basak Ezgi Sarac, Ozge Can Bostan, Gulseren Tuncay, Hayriye Akel Bilgic, Baran Erman, Umit Sahiner, Gul Karakaya, Ali Fuat Kalyoncu, Ebru Damadoglu, Cagatay Karaaslan","doi":"10.12932/AP-261024-1959","DOIUrl":"https://doi.org/10.12932/AP-261024-1959","url":null,"abstract":"<p><strong>Background: </strong>Nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD) is a clinical syndrome characterized by chronic rhinosinusitis with nasal polyposis (CRSwNP), adult-onset asthma and hypersensitivity to NSAIDs. Long-term aspirin treatment after desensitization (ATAD) is used for clinical improvement in N-ERD patients. However, information on the potential effect of ATAD on the platelet-neutrophil aggregates (PNA) level in N-ERD patients is highly limited.</p><p><strong>Objective: </strong>This study aimed to explore the impact of PNA on the pathogenesis of N-ERD and the potential effect of ATAD on N-ERD patient profiles from a platelet point-of-view.</p><p><strong>Methods: </strong>Sixty-one individuals were enrolled, including 16 N-ERD patients with ATAD (ATAD+), 15 N-ERD patients without ATAD (ATAD-), 15 aspirin-tolerant asthma (ATA) patients, and 15 healthy controls (HCs). Lipid mediators classical in N-ERD, including urinary-LTE4 (uLTE4), prostaglandin-D2 (PGD2), and prostaglandin-E2 (PGE2) were assessed by ELISA. Platelet activation was estimated based on expression levels of sP-selectin, CD40L, Platelet Factor-4 (PF4), RANTES, Thromboxane-A2 (TXA2), PAF, 12-HETE in plasma levels by ELISA; and PNA percentage by flow cytometry.</p><p><strong>Results: </strong>ATAD+; 12-HETE, and PF4 levels were remarkably low, while higher levels were determined in ATAD- and ATA groups. ATAD+; uLTE4 levels were positively correlated with 12-HETE. Another positive correlation was detected between sP-selectin and 12-HETE in ATAD-. Compared to HCs, it was found that among all N-ERD patients, significant increase in PNA.</p><p><strong>Conclusions: </strong>Plasma levels of PGE2, PF4, and 12-HETE appear to be affected by aspirin treatment. We believe that 12-HETE could play a significant role in the N-ERD pathogenesis by contributing to platelet activation.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of anti-elastin antibodies in a mouse model of asthma. 抗弹性蛋白抗体在哮喘小鼠模型中的作用
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-01 DOI: 10.12932/AP-020221-1053

Background: The role of anti-elastin antibody (Ab) in the lung is unclear, although they may be involved in chronic obstructive pulmonary disease (COPD). Recently, increased anti-elastin Ab levels were reported in asthma.

Objective: To elucidate the role of anti-elastin Ab in asthma, we created a murine asthma model. Anti-elastin Ab in the airway was neutralized by intratracheal administration of elastin peptide, and the inhibitory effects of anti-elastin Ab on airway remodeling were evaluated.

Methods: BALB/c mice were immunized with ovalbumin (OVA) on days 0 and 14. After immunization, the mice received booster OVA via inhalation twice per week for 9 weeks, and bronchoalveolar lavage fluid (BALF) and lung tissues were evaluated.

Results: In lung tissues, airway remodeling occurred after 9 weeks of OVA sensitization. Peak levels of anti-elastin Ab and eosinophils in BALF were detected after 3 weeks of OVA sensitization. Anti-elastin Ab and eosinophil levels in BALF were significantly reduced after 3 weeks by the neutralization of anti-elastin Ab. Peak transforming growth factor-β1 levels in BALF were detected at 3 weeks after OVA sensitization and were significantly reduced by the neutralization of anti-elastin Ab. Airway remodeling in lung tissues was also significantly inhibited by the neutralization of anti-elastin Ab.

Conclusions: In our murine asthma model, anti-elastin Ab was recruited to the airway by OVA-induced allergic inflammation. Airway remodeling was inhibited by the neutralization of anti-elastin Ab. Anti-elastin Ab may contribute to the progression of airway remodeling.

背景:尽管抗弹性蛋白抗体(Ab)可能与慢性阻塞性肺病(COPD)有关,但其在肺部的作用尚不明确。最近,有报道称哮喘患者的抗弹性蛋白抗体水平升高:为了阐明抗弹性蛋白抗体在哮喘中的作用,我们建立了一个小鼠哮喘模型。气管内注射弹性蛋白肽中和气道中的抗弹性蛋白抗体,并评估抗弹性蛋白抗体对气道重塑的抑制作用:在第 0 天和第 14 天用卵清蛋白(OVA)免疫 BALB/c 小鼠。免疫后,小鼠每周吸入两次加强型 OVA,持续 9 周,并对支气管肺泡灌洗液(BALF)和肺组织进行评估:结果:OVA致敏9周后,肺组织中的气道发生重塑。OVA致敏3周后,BALF中的抗弹性蛋白抗体和嗜酸性粒细胞水平达到峰值。3 周后,通过中和抗弹性蛋白抗体,BALF 中的抗弹性蛋白抗体和嗜酸性粒细胞水平明显降低。在OVA致敏3周后检测到BALF中转化生长因子-β1水平达到峰值,抗弹性蛋白抗体中和后可显著降低该水平。抗弹性蛋白抗体中和也能显著抑制肺组织中的气道重塑:结论:在我们的小鼠哮喘模型中,抗弹性蛋白抗体被 OVA 诱导的过敏性炎症招募到气道中。结论:在我们的小鼠哮喘模型中,抗弹性蛋白Ab被OVA诱导的过敏性炎症招募到气道,抗弹性蛋白Ab的中和抑制了气道重塑。抗弹性蛋白抗体可能有助于气道重塑的进展。
{"title":"The role of anti-elastin antibodies in a mouse model of asthma.","authors":"Tomoshige Wakayama, Kumiya Sugiyama, Shingo Tokita, Hajime Arifuku, Naotatsu Otsuji, Kei Sugitate, Takayoshi Owada, Kenya Koyama, Hirokuni Hirata, Masafumi Arima, Yasutsugu Fukushima","doi":"10.12932/AP-020221-1053","DOIUrl":"10.12932/AP-020221-1053","url":null,"abstract":"<p><strong>Background: </strong>The role of anti-elastin antibody (Ab) in the lung is unclear, although they may be involved in chronic obstructive pulmonary disease (COPD). Recently, increased anti-elastin Ab levels were reported in asthma.</p><p><strong>Objective: </strong>To elucidate the role of anti-elastin Ab in asthma, we created a murine asthma model. Anti-elastin Ab in the airway was neutralized by intratracheal administration of elastin peptide, and the inhibitory effects of anti-elastin Ab on airway remodeling were evaluated.</p><p><strong>Methods: </strong>BALB/c mice were immunized with ovalbumin (OVA) on days 0 and 14. After immunization, the mice received booster OVA via inhalation twice per week for 9 weeks, and bronchoalveolar lavage fluid (BALF) and lung tissues were evaluated.</p><p><strong>Results: </strong>In lung tissues, airway remodeling occurred after 9 weeks of OVA sensitization. Peak levels of anti-elastin Ab and eosinophils in BALF were detected after 3 weeks of OVA sensitization. Anti-elastin Ab and eosinophil levels in BALF were significantly reduced after 3 weeks by the neutralization of anti-elastin Ab. Peak transforming growth factor-β1 levels in BALF were detected at 3 weeks after OVA sensitization and were significantly reduced by the neutralization of anti-elastin Ab. Airway remodeling in lung tissues was also significantly inhibited by the neutralization of anti-elastin Ab.</p><p><strong>Conclusions: </strong>In our murine asthma model, anti-elastin Ab was recruited to the airway by OVA-induced allergic inflammation. Airway remodeling was inhibited by the neutralization of anti-elastin Ab. Anti-elastin Ab may contribute to the progression of airway remodeling.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"56-64"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39434837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Home-based up-dosing of wheat oral immunotherapy: Real-world effectiveness and predictive factor analysis. 基于家庭的小麦口服免疫疗法:实际效果和预测因素分析。
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-01 DOI: 10.12932/AP-130224-1783
Jiwon Kim, Minyoung Jung, Sehun Jang, Sanghee Shin, Jeongmin Song, Sukyung Kim, Sukyung Kim, Ji Young Lee, Hyun Mi Kim, Yeonghee Kim, Min Hee Lee, Su Jin Lee, Minji Kim, Jihyun Kim, Kangmo Ahn

Background: Wheat allergy is one of the most prevalent allergens in Korea, decreasing quality of life and causing nutritional repercussions.

Objective: We aimed to investigate the efficacy and safety of the home-based wheat oral immunotherapy (OIT) using wheat noodles in children with a wheat allergy.

Methods: We conducted a retrospective study involving 72 children aged 3 to 17 years diagnosed with a wheat allergy. Patients received wheat OIT using wheat noodles (n = 50) and were compared with a historical control group (n = 22). Baseline characteristics, adverse events, and immunological changes were assessed. Predictors of successful desensitization were identified using logistic regression analysis.

Results: Among 50 patients completing the up-dosing phase, 82.0% achieved desensitization to 2,400 mg of wheat protein, compared to 4.5% in the control group (p < 0.001). During the up-dosing period, the median number of adverse reactions per person was 2, and anaphylaxis occurred in 30.0% (15/50). However, there were no life-threatening adverse events. In multivariable analysis, the presence of asthma (adjusted odds ratio [aOR], 8.88; 95% confidence interval [CI], 1.10-71.97; p = 0.041) and a higher ratio of specific IgE (sIgE) to ω-5-gliadin and total IgE (aOR 19.09, 95%CI 1.21-300.80, p = 0.036) were significantly associated with treatment outcomes of wheat OIT.

Conclusion: Our study showed the safety and efficacy of home-based wheat OIT using boiled noodles in Korean children with wheat allergies. Careful consideration is warranted for patients with elevated baseline sIgE to ω-5-gliadin to total IgE ratio and a history of asthma.

背景:小麦过敏是韩国最常见的过敏原之一,会降低生活质量并对营养造成影响:我们的目的是调查使用小麦面条对小麦过敏儿童进行家庭小麦口服免疫疗法(OIT)的有效性和安全性:我们进行了一项回顾性研究,涉及 72 名被诊断为小麦过敏的 3 至 17 岁儿童。患者接受了使用小麦面条的小麦 OIT(50 人),并与历史对照组(22 人)进行了比较。对基线特征、不良事件和免疫学变化进行了评估。通过逻辑回归分析确定了成功脱敏的预测因素:结果:在完成增量给药阶段的 50 名患者中,82.0% 的患者对 2400 毫克小麦蛋白实现了脱敏,而对照组仅为 4.5%(p < 0.001)。在增加剂量期间,每人的不良反应中位数为 2 次,30.0% 的患者(15/50)出现过敏性休克。不过,没有出现危及生命的不良反应。在多变量分析中,是否患有哮喘(调整赔率[aOR],8.88;95% 置信区间[CI],1.10-71.97;p = 0.041)以及特异性 IgE(sIgE)与ω-5-花生蛋白和总 IgE 的比率是否较高(aOR 19.09,95%CI 1.21-300.80,p = 0.036)与小麦 OIT 的治疗结果显著相关:我们的研究表明,在韩国小麦过敏儿童中使用煮熟的面条进行家庭小麦 OIT 既安全又有效。对于ω-5-gliadin sIgE 与总 IgE 比值基线升高且有哮喘病史的患者,应慎重考虑。
{"title":"Home-based up-dosing of wheat oral immunotherapy: Real-world effectiveness and predictive factor analysis.","authors":"Jiwon Kim, Minyoung Jung, Sehun Jang, Sanghee Shin, Jeongmin Song, Sukyung Kim, Sukyung Kim, Ji Young Lee, Hyun Mi Kim, Yeonghee Kim, Min Hee Lee, Su Jin Lee, Minji Kim, Jihyun Kim, Kangmo Ahn","doi":"10.12932/AP-130224-1783","DOIUrl":"10.12932/AP-130224-1783","url":null,"abstract":"<p><strong>Background: </strong>Wheat allergy is one of the most prevalent allergens in Korea, decreasing quality of life and causing nutritional repercussions.</p><p><strong>Objective: </strong>We aimed to investigate the efficacy and safety of the home-based wheat oral immunotherapy (OIT) using wheat noodles in children with a wheat allergy.</p><p><strong>Methods: </strong>We conducted a retrospective study involving 72 children aged 3 to 17 years diagnosed with a wheat allergy. Patients received wheat OIT using wheat noodles (n = 50) and were compared with a historical control group (n = 22). Baseline characteristics, adverse events, and immunological changes were assessed. Predictors of successful desensitization were identified using logistic regression analysis.</p><p><strong>Results: </strong>Among 50 patients completing the up-dosing phase, 82.0% achieved desensitization to 2,400 mg of wheat protein, compared to 4.5% in the control group (p < 0.001). During the up-dosing period, the median number of adverse reactions per person was 2, and anaphylaxis occurred in 30.0% (15/50). However, there were no life-threatening adverse events. In multivariable analysis, the presence of asthma (adjusted odds ratio [aOR], 8.88; 95% confidence interval [CI], 1.10-71.97; p = 0.041) and a higher ratio of specific IgE (sIgE) to ω-5-gliadin and total IgE (aOR 19.09, 95%CI 1.21-300.80, p = 0.036) were significantly associated with treatment outcomes of wheat OIT.</p><p><strong>Conclusion: </strong>Our study showed the safety and efficacy of home-based wheat OIT using boiled noodles in Korean children with wheat allergies. Careful consideration is warranted for patients with elevated baseline sIgE to ω-5-gliadin to total IgE ratio and a history of asthma.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"97-105"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elevation of anti-elastin antibody in patients with asthma. 哮喘患者体内的抗弹性蛋白抗体升高。
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-01 DOI: 10.12932/AP-010221-1052

Background: It is often difficult to differentiate between asthma and chronic obstructive pulmonary disease (COPD), and useful biomarkers are needed for accurate diagnosis.

Objective: We evaluated anti-elastin antibody to identify useful biomarkers for differentiating between a diagnosis of asthma and COPD.

Methods: Patients with asthma (male to female ratio = 10/13; mean age, 67.3 years), COPD (16/0; 74.8 years) and controls (8/4; 72.3 years) were enrolled. Samples from sputum and serum were collected and levels of anti-elastin Ab were measured.

Results: The levels of anti-elastin Ab in sputum were significantly higher in asthma (11.4 ± 7.16 μg/mL) than in COPD (5.82 ± 5.16 μg/mL; P < 0.01), and serum levels in asthma (67.4 ± 29.7 μg/mL) were also significantly higher than in COPD or controls (45.0 ± 12.8 μg/mL; P < 0.05, 38.6 ± 10.4 μg/mL; P < 0.01, respectively). Anti-elastin Ab in sputum showed a positive correlation with smoking in asthma (r2 = 0.218, P < 0.05). However, no significant differences were observed in the levels of anti-elastin Ab and eosinophils, asthma phenotypes, inhaled corticosteroids, or severity in patients with asthma. Elastin was strongly expressed under the airway basement membrane in asthma compared with COPD or the healthy control.

Conclusions: Anti-elastin Ab in sputum could be a useful biomarker for COPD and asthma in ever-smokers. In asthma, anti-elastin Ab was recruited to the airways by both airway allergic inflammation and smoking, and it may contribute to the progression of airway remodeling via autoimmune inflammation, but not emphysema, in COPD.

背景:通常很难区分哮喘和慢性阻塞性肺病(COPD),因此需要有用的生物标志物来进行准确诊断:我们评估了抗弹性蛋白抗体,以确定区分哮喘和慢性阻塞性肺疾病诊断的有用生物标志物:方法:我们招募了哮喘患者(男女比例=10/13;平均年龄 67.3 岁)、慢性阻塞性肺病患者(16/0;74.8 岁)和对照组患者(8/4;72.3 岁)。采集痰液和血清样本并测定抗弹性蛋白抗体水平:哮喘患者痰中抗弹性蛋白抗体水平(11.4 ± 7.16 μg/mL)明显高于慢性阻塞性肺病患者(5.82 ± 5.16 μg/mL;P <0.01),哮喘患者血清中抗弹性蛋白抗体水平(67.4 ± 29.7 μg/mL)也明显高于COPD或对照组(分别为45.0 ± 12.8 μg/mL;P < 0.05,38.6 ± 10.4 μg/mL;P < 0.01)。痰中的抗弹性蛋白抗体与哮喘患者吸烟呈正相关(r2 = 0.218,P < 0.05)。然而,在哮喘患者的抗弹性蛋白抗体和嗜酸性粒细胞水平、哮喘表型、吸入皮质类固醇或严重程度方面均未观察到明显差异。与慢性阻塞性肺病或健康对照组相比,哮喘患者气道基底膜下的弹性蛋白表达较强:结论:痰中的抗弹性蛋白抗体可作为慢性阻塞性肺病和哮喘的有效生物标志物。在哮喘患者中,气道过敏性炎症和吸烟都会将抗弹性蛋白Ab招募到气道中,它可能会通过自身免疫性炎症导致气道重塑,但不会导致慢性阻塞性肺病患者的肺气肿。
{"title":"Elevation of anti-elastin antibody in patients with asthma.","authors":"Shingo Tokita, Kumiya Sugiyama, Tomoshige Wakayama, Hajime Arifuku, Naotatsu Otsuji, Kei Sugitate, Takayoshi Owada, Kenya Koyama, Hirokuni Hirata, Masafumi Arima, Yoshihiko Ueda, Yasutsugu Fukushima","doi":"10.12932/AP-010221-1052","DOIUrl":"10.12932/AP-010221-1052","url":null,"abstract":"<p><strong>Background: </strong>It is often difficult to differentiate between asthma and chronic obstructive pulmonary disease (COPD), and useful biomarkers are needed for accurate diagnosis.</p><p><strong>Objective: </strong>We evaluated anti-elastin antibody to identify useful biomarkers for differentiating between a diagnosis of asthma and COPD.</p><p><strong>Methods: </strong>Patients with asthma (male to female ratio = 10/13; mean age, 67.3 years), COPD (16/0; 74.8 years) and controls (8/4; 72.3 years) were enrolled. Samples from sputum and serum were collected and levels of anti-elastin Ab were measured.</p><p><strong>Results: </strong>The levels of anti-elastin Ab in sputum were significantly higher in asthma (11.4 ± 7.16 μg/mL) than in COPD (5.82 ± 5.16 μg/mL; P < 0.01), and serum levels in asthma (67.4 ± 29.7 μg/mL) were also significantly higher than in COPD or controls (45.0 ± 12.8 μg/mL; P < 0.05, 38.6 ± 10.4 μg/mL; P < 0.01, respectively). Anti-elastin Ab in sputum showed a positive correlation with smoking in asthma (r2 = 0.218, P < 0.05). However, no significant differences were observed in the levels of anti-elastin Ab and eosinophils, asthma phenotypes, inhaled corticosteroids, or severity in patients with asthma. Elastin was strongly expressed under the airway basement membrane in asthma compared with COPD or the healthy control.</p><p><strong>Conclusions: </strong>Anti-elastin Ab in sputum could be a useful biomarker for COPD and asthma in ever-smokers. In asthma, anti-elastin Ab was recruited to the airways by both airway allergic inflammation and smoking, and it may contribute to the progression of airway remodeling via autoimmune inflammation, but not emphysema, in COPD.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"49-55"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39172752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prick and intradermal skin tests in patients with severe hymenoptera sting allergy using commercial versus in-house allergen extracts. 使用商品与自制过敏原提取物对严重绣线菊蜇伤过敏患者进行刺穿和皮内皮肤试验。
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-01 DOI: 10.12932/AP-151120-0995

Background: Fire ant, honey bee, and wasp allergen extracts are useful in the diagnosis and treatment of severe Hymenoptera allergic patients.

Objective: To evaluate the result of skin prick test (SPT) and intradermal test (ID) compared between local and commercial insect allergen extracts in patients with severe Hymenoptera sting allergy.

Methods: SPT and ID using local and commercial insect allergen extracts were performed. Specific IgE (sIgE) to honey bee, wasp, and fire ant; component-resolved diagnosis (CRD); (rApi m1, rApi m2, rApi m3, rApi m5, rApi m10, rVes v5, rPol d5, and rVes v1); and, cross-reactive carbohydrate determinant (CCD) were performed.

Results: Twenty-seven patients were included. Twenty-five had anaphylaxis, and 2 had severe systemic skin reaction. Positive skin test (SPT and/or ID) result from local and commercial allergen extracts was 74% vs. 67% for fire ant, 48% vs. 59% for honey bee, and 52% vs. 74% for yellowjacket. Local and commercial allergen extracts showed substantial agreement for fire ant (k = 0.647, p = 0.001) and honey bee (k = 0.632, p = 0.001), and moderate agreement for wasp (k = 0.547, p = 0.001). When compared with sIgE subtracted with CCD and/or CRD, skin test results of local fire ant allergen extract showed higher sensitivity (87% vs. 67%), specificity (42% vs. 33%), and accuracy (67% vs. 52%) than commercial extract. Commercial honey bee and wasp showed higher sensitivity (62% vs. 50%, 85% vs. 65%) and accuracy (63% vs. 52%, 78% vs. 70%), respectively.

Conclusions: SPT and ID with local or commercial insect venoms could help in confirming and/or identifying the causative insects.

背景:火蚁、蜜蜂和黄蜂过敏原提取物可用于严重膜翅目昆虫过敏症患者的诊断和治疗:评估本地和商业昆虫过敏原提取物对严重膜翅目昆虫蛰伤过敏患者的皮肤点刺试验(SPT)和皮内试验(ID)的结果比较:方法:使用本地和商业昆虫过敏原提取物进行 SPT 和 ID 试验。方法:使用本地和商业昆虫过敏原提取物进行 SPT 和 ID,检测蜜蜂、黄蜂和火蚁的特异性 IgE(sIgE);成分解析诊断(CRD);(rApi m1、rApi m2、rApi m3、rApi m5、rApi m10、rVes v5、rPol d5 和 rVes v1);以及交叉反应碳水化合物决定簇(CCD):结果:共纳入 27 名患者。结果:共纳入 27 例患者,其中 25 例出现过敏性休克,2 例出现严重的全身性皮肤反应。本地和商业过敏原提取物的皮肤测试(SPT 和/或 ID)阳性率分别为:火蚁 74% 对 67%,蜜蜂 48% 对 59%,黄蜂 52% 对 74%。本地和商业过敏原提取物在火蚁(k = 0.647,p = 0.001)和蜜蜂(k = 0.632,p = 0.001)方面显示出很大的一致性,在黄蜂(k = 0.547,p = 0.001)方面显示出中等程度的一致性。与用 CCD 和/或 CRD 减去的 sIgE 相比,本地火蚁过敏原提取物皮试结果的灵敏度(87% 对 67%)、特异性(42% 对 33%)和准确性(67% 对 52%)均高于商品提取物。商品蜜蜂和黄蜂的灵敏度(62% 对 50%,85% 对 65%)和准确度(63% 对 52%,78% 对 70%)分别更高:使用本地或商业昆虫毒液进行 SPT 和 ID 有助于确认和/或鉴别致病昆虫。
{"title":"Prick and intradermal skin tests in patients with severe hymenoptera sting allergy using commercial versus in-house allergen extracts.","authors":"Nualanong Visitsunthorn, Kittipos Visitsunthorn, Nitat Sookrung, Witchaya Srisuwatchari, Punchama Pacharn, Orathai Jirapongsananuruk","doi":"10.12932/AP-151120-0995","DOIUrl":"10.12932/AP-151120-0995","url":null,"abstract":"<p><strong>Background: </strong>Fire ant, honey bee, and wasp allergen extracts are useful in the diagnosis and treatment of severe Hymenoptera allergic patients.</p><p><strong>Objective: </strong>To evaluate the result of skin prick test (SPT) and intradermal test (ID) compared between local and commercial insect allergen extracts in patients with severe Hymenoptera sting allergy.</p><p><strong>Methods: </strong>SPT and ID using local and commercial insect allergen extracts were performed. Specific IgE (sIgE) to honey bee, wasp, and fire ant; component-resolved diagnosis (CRD); (rApi m1, rApi m2, rApi m3, rApi m5, rApi m10, rVes v5, rPol d5, and rVes v1); and, cross-reactive carbohydrate determinant (CCD) were performed.</p><p><strong>Results: </strong>Twenty-seven patients were included. Twenty-five had anaphylaxis, and 2 had severe systemic skin reaction. Positive skin test (SPT and/or ID) result from local and commercial allergen extracts was 74% vs. 67% for fire ant, 48% vs. 59% for honey bee, and 52% vs. 74% for yellowjacket. Local and commercial allergen extracts showed substantial agreement for fire ant (k = 0.647, p = 0.001) and honey bee (k = 0.632, p = 0.001), and moderate agreement for wasp (k = 0.547, p = 0.001). When compared with sIgE subtracted with CCD and/or CRD, skin test results of local fire ant allergen extract showed higher sensitivity (87% vs. 67%), specificity (42% vs. 33%), and accuracy (67% vs. 52%) than commercial extract. Commercial honey bee and wasp showed higher sensitivity (62% vs. 50%, 85% vs. 65%) and accuracy (63% vs. 52%, 78% vs. 70%), respectively.</p><p><strong>Conclusions: </strong>SPT and ID with local or commercial insect venoms could help in confirming and/or identifying the causative insects.</p>","PeriodicalId":8552,"journal":{"name":"Asian Pacific journal of allergy and immunology","volume":" ","pages":"40-48"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39172756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thymocytes induce renal tubular epithelial cells to undergo the epithelial-to-mesenchymal transition. 胸腺细胞诱导肾小管上皮细胞发生上皮到间质的转变。
IF 2.3 4区 医学 Q3 ALLERGY Pub Date : 2025-03-01 DOI: 10.12932/AP-210221-1075

Background: Renal tubulointerstitial fibrosis is known to occur as a result of epithelial cell transformation into myofibroblasts via the epithelial-to-mesenchymal transition (EMT) process. It has been reported that macrophages, regulatory T (Treg) cells, and gamma delta T (γδ T) cells can promote fibrosis via EMT in vivo.

Objective: Our study intended to detect whether thymocytes can induce renal tubular cells to undergo the EMT.

Methods: Rat thymocytes were activated by phytohemagglutinin and concanavalin A. The rat renal tubular epithelial cells (NRK-52E) were incubated in a conditioned medium harvested from activated thymocytes or co-cultured with freshly isolated thymocytes for 48 hours. Real-time reverse transcription-polymerase chain reaction, immunofluorescence, and western blotting analysis were used to test the expression of the epithelial and mesenchymal markers in NRK-52E cells. Scratch assay was designed to test the cell migration abilities of NRK-52E cells. Student's t test and oneway analysis of variance test were used for statistical analysis.

Results: The combined stimulation with phytohemagglutinin and concanavalin A activated the primary isolated rat thymocytes. After treatment with conditioned medium or freshly isolated thymocytes, the expression levels of cytokeratin 19 and E-cadherin were downregulated in NRK-52E cells, while the mRNA and protein expression levels of alpha-smooth muscle actin, desmin, and vimentin were upregulated (P < 0.05). We found that the cell migration abilities of the induced NRK-52E cells were significantly improved.

Conclusions: Both activated rat thymocytes (more percentage of CD8+ T cells) and freshly isolated thymocytes have promoting effects on the EMT of NRK-52E cells.

背景:众所周知,肾小管间质纤维化是上皮细胞通过上皮-间质转化(EMT)过程转变为肌成纤维细胞的结果。据报道,巨噬细胞、调节性 T(Treg)细胞和γδT(γδT)细胞可通过 EMT 促进体内纤维化:我们的研究旨在检测胸腺细胞是否能诱导肾小管细胞发生 EMT:大鼠肾小管上皮细胞(NRK-52E)在从活化的胸腺细胞中获得的条件培养基中或与新鲜分离的胸腺细胞共培养48小时。采用实时逆转录聚合酶链反应、免疫荧光和 Western 印迹分析检测 NRK-52E 细胞中上皮和间质标记物的表达。划痕实验用于检测 NRK-52E 细胞的迁移能力。统计分析采用学生 t 检验和单向方差分析:结果:植物血凝素和 concanavalin A 的联合刺激激活了原代离体大鼠胸腺细胞。经条件培养基或新鲜分离的胸腺细胞处理后,NRK-52E细胞中细胞角蛋白19和E-cadherin的表达水平下调,而α-平滑肌肌动蛋白、desmin和波形蛋白的mRNA和蛋白表达水平上调(P < 0.05)。我们发现,诱导的 NRK-52E 细胞的细胞迁移能力明显提高:结论:活化的大鼠胸腺细胞(CD8+ T 细胞比例更高)和新鲜分离的胸腺细胞都对 NRK-52E 细胞的 EMT 有促进作用。
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Asian Pacific journal of allergy and immunology
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