[Comparative immunohistochemistry study of different antibodies clones for detection of breast cancer markers (estrogen receptor, progesterone receptor, HER2/neu, Ki-67)].

Q4 Medicine Arkhiv patologii Pub Date : 2024-01-01 DOI:10.17116/patol20248602114

D V Kalinin, D V Samoylova, N V Danilova, N Yu Germanovich, O A Kuznetsova, L E Zavalishina

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Abstract

Objective: A comparative study of detection of breast cancer markers (estrogen receptors, progesterone receptors, HER2/neu, Ki-67) by immunohistochemical method with antibodies produced by PrimeBioMed (Russia) and antibodies produced by Roche Ventana (USA).

Material and methods: Surgical specimens and biopsies from 37 patients with invasive breast cancer were used. Sections were stained with antibodies of clones ER SP1 and GM030, PR 1E2 and PBM-5B8, HER2/neu 4B5 and PBM-46A6, Ki-67 30-9 and GM010.

Results: There was a high positive and significant correlation between the immunohistochemistry results and antibodies of the clones ER-SP1 and GM030, PR1E2 and PBM-5B8, HER2/neu4B5 and PBM-46A6, Ki-67 30-9 and GM010.

Conclusion: The study showed the possibility of using antibodies of clones GM030, HER2/neu 4B5, PBM-46A6, GM010 (PrimeBioMed) on the Ventana Bench Marck Ultra automatic immunostainer using the detection system UltraView Universal DAB Detection Kit.

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[不同抗体克隆检测乳腺癌标志物（雌激素受体、孕激素受体、HER2/neu、Ki-67）的免疫组化比较研究]。

目的：使用俄罗斯 PrimeBioMed 公司生产的抗体和美国罗氏 Ventana 公司生产的抗体，通过免疫组化方法检测乳腺癌标志物（雌激素受体、孕激素受体、HER2/neu、Ki-67）的比较研究：采用 37 例浸润性乳腺癌患者的手术标本和活组织切片。用ER SP1和GM030、PR 1E2和PBM-5B8、HER2/neu 4B5和PBM-46A6、Ki-67 30-9和GM010克隆抗体对切片进行染色：结果：免疫组化结果与克隆ER-SP1和GM030、PR1E2和PBM-5B8、HER2/neu4B5和PBM-46A6、Ki-67 30-9和GM010的抗体之间存在高度正相关和显著相关：研究表明，可以在 Ventana Bench Marck Ultra 自动免疫印迹仪上使用克隆 GM030、HER2/neu 4B5、PBM-46A6、GM010（PrimeBioMed）的抗体，并使用 UltraView 通用 DAB 检测试剂盒进行检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arkhiv patologii Medicine-Pathology and Forensic Medicine

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The journal deals with original investigations on pressing problems of general pathology and pathologic anatomy, newest research methods, major issues of the theory and practice as well as problems of experimental, comparative and geographic pathology. To inform readers latest achievements of Russian and foreign medicine the journal regularly publishes editorial and survey articles, reviews of the most interesting Russian and foreign books on pathologic anatomy, new data on modern methods of investigation (histochemistry, electron microscopy, autoradiography, etc.), about problems of teaching, articles on the history of pathological anatomy development both in Russia and abroad.