[Study on the antimicrobial effect of new bioactive glass against common bacteria in apical periodontitis of deciduous teeth].

Q4 Medicine 上海口腔医学 Pub Date : 2024-02-01

Ying Fang, Wen-Yan Huang, Guo-Hou Miao, Si Meng, Bo Peng, Su-Juan Zeng

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引用次数: 0

Abstract

Purpose: To study the antimicrobial effect of different concentrations of new bioactive glass(BG) on common bacteria in apical periodontitis of deciduous teeth.

Methods: The diameter (mm) of the inhibitory rings formed after treatment of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum with the new bioactive glass was detected and observed by paper diffusion method, and the minimal inhibitory concentration(MIC), minimal bactericidal concentration (MBC) and minimal biofilm eradication concentration (MBEC) of E. faecalis, P. gingivalis and C. pseudomallei were determined. The mixed plaques of the three bacteria were treated with 20, 40, 60 and 80 mg/mL of the new bioactive glass for 24 h. The results were analyzed by laser confocal microscopy. The antibacterial effect of the new bioactive glass on the mixed plaque was observed by confocal laser scanning microscopy (CLSM). Statistical analysis was performed with GraphPad Prism 10.0 software.

Results: The new bioactive glass showed strong antibacterial potential against the common bacteria of apical periodontitis; the MBEC of the new bioactive glass on the plaque was significantly greater than MIC and MBC of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, and as the concentration of the new bioactive glass increased, the number of dead bacteria in the mixed plaque increased, and there was significant difference from that of the blank control group (P＜0.05).

Conclusions: The novel bioactive glass shows significant antibacterial efficacy against Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, which are the common bacteria in apical periodontitis of deciduous teeth.

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[新型生物活性玻璃对乳牙根尖牙周炎常见细菌的抗菌效果研究]。

目的：研究不同浓度的新型生物活性玻璃（BG）对乳牙根尖牙周炎常见细菌的抗菌效果：方法：用纸片扩散法检测和观察粪肠球菌、牙龈卟啉单胞菌和核酸梭菌经新型生物活性玻璃处理后形成的抑菌圈的直径（mm），并测定粪肠球菌、牙龈卟啉单胞菌和核酸梭菌的最小抑菌浓度（MIC）、最小杀菌浓度（MBC）和最小生物膜根除浓度（MBEC）。用 20、40、60 和 80 毫克/毫升的新型生物活性玻璃处理三种细菌的混合斑块 24 小时。激光共聚焦扫描显微镜（CLSM）观察了新型生物活性玻璃对混合斑块的抗菌效果。使用 GraphPad Prism 10.0 软件进行统计分析：结果：新型生物活性玻璃对牙周炎根尖常见细菌有较强的抗菌作用；新型生物活性玻璃对牙菌斑的MBEC显著大于粪肠球菌、牙龈卟啉单胞菌和核酸梭菌的MIC和MBC，且随着新型生物活性玻璃浓度的增加，混合菌斑中死亡细菌数增加，与空白对照组相比差异有显著性（P＜0.05）：新型生物活性玻璃对乳牙根尖牙周炎中常见的粪肠球菌、牙龈卟啉单胞菌和核酸梭菌有明显的抗菌效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

上海口腔医学 Medicine-Medicine (all)

CiteScore

0.30

自引率

0.00%

发文量

5299

期刊介绍： "Shanghai Journal of Stomatology (SJS)" is a comprehensive academic journal of stomatology directed by Shanghai Jiao Tong University and sponsored by the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. The main columns include basic research, clinical research, column articles, clinical summaries, reviews, academic lectures, etc., which are suitable for reference by clinicians, scientific researchers and teaching personnel at all levels engaged in oral medicine.