上海口腔医学最新文献

Purpose: To compare the trueness of the tissue surface of the complete denture base among the digital cutting complete denture, the digital 3D printing complete denture and the conventional complete denture.

Methods: Three edentulous patients were selected, and three complete dentures were made for each case. Cutting dentures (cutting group), 3D printed dentures(printing group) were considered as the experimental groups, traditional dentures (traditional group) were considered as the control group. The tissue surface of complete denture model and denture base were scanned and their 3D data were obtained. Geomagic software was used to fit the model information with the tissue surface information of the denture base. A total of 8 regions were selected in the upper and lower complete dentures, and 10 points were randomly selected in each region. The average value of the absolute deviation value(Dm) of each region was calculated. SPSS 22.0 software package was used to analyze the Dm in a total of 24 regions of the three pairs of dentures.

Results: The absolute mean deviation was (0.056±0.025) mm in the cutting group, (0.103±0.038) mm in the printing group, and (0.096±0.039) mm in the traditional group, which were significantly different among the three groups(P＜0.05). The trueness of the ablation group was better than that of the conventional group, and the difference was significant (P＜0.05). The trueness of the printing group was worse than that of the traditional group, and the difference was not significant(P＞0.05).

Conclusions: The trueness of cutting dentures is better than that of traditional dentures, while the trueness of 3D printed dentures is not significantly better than that of traditional dentures.

Purpose: To explore the association between tumor necrosis factor-associated apoptosis-inducing ligand (TRAIL) gene polymorphism and susceptibility to diabetic periodontitis.

Methods: A total of 150 patients with type 2 diabetes were selected from September 2022 to September 2023. The patients were divided into combined group(n=50), non-combined group (n=50) and control group (n=50) according to whether they had periodontitis. Clinical medical records of all subjects were collected, TRAIL G1525A and C1595T gene polymorphisms were detected, and the influencing factors of type 2 diabetic periodontitis were analyzed. Statistical analysis was performed with SPSS 19.0 software package.

Results: In combined and non-combined group, the fasting blood glucose(FBG), loss of attachment(AL), gingival crevicular bleeding index (SBI) and blood calcium were significantly lower than those in the control group, and HbA1c and serum alkaline phosphatase(ALP) were significantly higher than those in the control group(P＜0.05). The GG gene frequency of TRAIL G1525A gene in the combined group was significantly higher than that in the non-combined group and control group(P＜0.05). There was no significant difference in genotype distribution of TRAIL C1595T among three groups(P＞0.05).HbA1c, AL, SBI, ALP and TRAIL G1525A polymorphisms were risk factors for periodontitis(P＜0.05). The distribution of TRAIL G1525ALeuGGin with moderate and severe periodontitis was significantly different from that with mild periodontitis (P＜0.05).

Conclusions: TRAIL G1525A gene is associated with the susceptibility of diabetic periodontitis, and GG genotype patients with type 2 diabetes have a higher risk of periodontitis.

Purpose: To explore the effects of personalized oral stents in intensity-modulated radiation therapy (IMRT) treatment.

Methods: Totally 108 patients with nasopharyngeal carcinoma treated with IMRT at Nanjing Gaochun People's Hospital from January 2021 to December 2023 were selected. Using a random number table method, the patients were divided into experimental group (n=54) using personalized oral stents and control group (n=54) using simple mouthpieces. The dosimetry of clinical target volumes (CTV), normal oral tissues, conformity index (CI), homogeneity index (HI), pain level evaluated by visual analogue scale(VAS ), grades of oral mucositis, xerostomia scores, taste scores, oral health impact profile (OHIP) scores and incidence of complications(dental caries, xerostomia, taste disorders, oral candidiasis, osteomyelitis of the jaw) were compared between the two groups. SPSS 23.0 software package was used for statistical analysis.

Results: There was no significant difference between the two groups in average received dose and CI, HI values on CTV and the tongue(P＞0.05); however, the experimental group showed a lower average received dose on the mandible, mandibular teeth, and bilateral parotid glands compared to the control group(P＜0.05). After treatment, the experimental group had significantly lower scores in VAS, grades of oral mucositis, xerostomia evaluation, OHIP and frequency of complications (P＜0.05). The taste scores in the experimental group were significantly higher than that in the control group (P＜0.05).

Conclusions: Personalized oral stents significantly reduce the radiation dose to oral sites in IMRT treatment, decrease pain and other oral complications, and improve patients' quality of life.

Purpose: To explore the differences between plaque biofilms of children with different caries activities via metabolomics.

Methods: A case-control study was conducted to investigate the oral health-related behaviors of children in caries-free (CF), low level of early childhood caries(LECC) and high level of early childhood caries (HECC) groups and to collect supragingival plaque biofilms. Untargeted metabolomics was used to detect the compositions of plaque biofilm metabolites in three groups. Orthogonal partial least squares discriminant analysis, volcano plot and heat map were used to analyze the significant differential metabolites between groups with SPSS 26.0 software package.

Results: A total of 102 children aged 36 months were included in this study, including 34 children in each group. A total of 660 metabolites belonging to 11 categories were detected. There were statistical differences of 6 metabolites between LECC group and CF group, and 22 metabolites showed statistical differences between HECC group and CF group(P＜0.05). Cys Cys Cys Gln, Cys Gln Cys Cys and 5-O-p-coumaroylnigrumin were the common differential metabolites between children with different levels of dental caries.

Conclusions: There are differences in the compositions of plaque biofilms between children with different caries activities and healthy children. Peptides are important differential metabolites within plaque biofilms, also related to oral health.

Purpose: To analyze the effect of n-HA/chitosan/minocycline composite scaffold in the animal model of peri-implant inflammatory bone defect.

Methods: Twelve healthy adult male beagle dogs were selected to construct the model of peri-implant inflammatory bone defect. The control group(n=6) underwent bone regeneration by alveolar self-healing without any treatment in the bone defect area. In the experimental group(n=6), n-HA/chitosan/minocycline composite scaffold prepared was implanted into the bone defect site. Periodontitis, bone defect, pathological parameters, bone mineral density(BMD) and new bone formation rate were recorded in two groups of animal models. Statistical analysis was performed with SPSS 26.0 software package.

Results: There was no significant difference in the horizontal width and vertical depth of bone defect between the control group and the experimental group before treatment (P＞0.05). At 4, 8 and 12 weeks after treatment, the horizontal width and vertical depth of the experimental group were significantly lower than those before treatment and the control group(P＜0.05). There was no significant difference in probing depth(PD), sulcus bleeding index(SBI) and plaque index(PLI) between the control group and the experimental group before treatment(P＞0.05). PD, SBI and PLI in the experimental group were significantly higher than those before treatment and in the control group at 4, 8 and 12 weeks after treatment(P＜0.05). BMD value, new bone height and new bone formation rate of the experimental group were significantly higher than those of the control group at 4, 8 and 12 weeks after treatment(P＜0.05).

Conclusions: n-HA/chitosan/minocycline composite scaffold shows a good effect in the model of peri-implant inflammatory bone defect, which can promote bone osteointegration and bone regeneration in the peri-implant bone defect area.

Purpose: To assess the impact of retrograde dissection of the facial nerve along the mandibular margin on the postoperative quality of life in patients with benign superficial parotid tumors.

Methods: One hundred and sixteen patients who underwent surgical treatment for benign superficial parotid tumors at Qingdao Central Hospital from January 2020 to January 2023 were involved. The patients were randomly allocated into two groups, with 58 patients in each group using the touch ball method.The control group underwent antegrade dissection of the facial nerve during surgery, while the experimental group underwent retrograde dissection along the mandibular margin. Surgical duration, intraoperative blood loss, incision length and hospital stay between the two groups were compared. Postoperative pain levels were recorded at 1, 3, 7 and 14 days after surgery. Chewing function was assessed at 15 days, 1 month, 3 months and 6 months postoperatively. House-Brackmann scale (H-B) and scar cosmesis assessment and rating (SCAR) scale were used to evaluate facial nerve function and aesthetic outcomes at 3 days and 6 months postoperatively. The quality of life of the two groups of patients was compared before and 6 months after surgery. Complications were recorded including temporary facial paralysis, periaural numbness, salivate fistula and Frey syndrome. SPSS 28.0 software package was used for statistical analysis.

Results: The operative time of the experimental group was significantly higher than that of the control group, intraoperative blood loss, incision length and postoperative pain score of the experimental group were significantly lower than those of the control group. Masticatory efficiency, facial nerve function, scar aesthetics and quality of life of the experimental group were significantly higher than those of the control group, while the incidence of complications was significantly decreased than those of the control group(P＜0.05).

Conclusions: The use of retrograde dissection of facial nerve along the mandibular marginal branch during surgery for benign parotid gland is helpful to reduce intraoperative blood loss and postoperative pain, and improve patients' quality of life.

Purpose: To explore the application effect of mind mapping-assisted flipped classroom in clinical endodontics teaching.

Methods: A total of 41 fifth-year undergraduates majoring in stomatology from School of Stomatology, Tongji University, finishing their clinical internship at Departments of Endodontics from October 2023 to March 2024, were randomly divided into control group (traditional teaching group, n=21) and experimental group (flipped classroom teaching group, n=20). Theoretical assessments were conducted on the day of entering the department and at the end of eight weeks in the department. Mini-CEX assessments were conducted in the third week and at the end of the eighth week (including indicators such as medical interviews, clinical examinations, humanistic care, clinical judgment, health education, clinical operations, and overall clinical capabilities). The scores of these two assessment indicators were used to evaluate the mastery extent of basic knowledge and clinical abilities of the two groups of students, and teaching satisfaction questionnaire survey was conducted. SPSS 22.0 software package was used for data analysis.

Results: In terms of theoretical assessment results, there was no significant difference between the two groups in the average scores of the first assessment. The average scores of both groups were better than those of the first assessment, and the average scores of the experimental group were significantly higher than those of the control group. In terms of mini-CEX assessment results, at the first assessment, the experimental group was better than the control group in two dimensions of clinical examination and clinical judgment, but there was no significant difference between the two groups in the other five dimensions. In the second assessment, the results of the two groups in 7 dimensions were higher than those of the first assessment, and the scores of the experimental group in 5 dimensions of medical interview, clinical examination, clinical judgment, clinical operation and overall clinical ability were significantly higher than those of the control group.

Conclusions: Mind mapping-assisted flipped classroom teaching can significantly improve the subjective initiative of undergraduate students during clinical internships, enhancing their mastery extent of theoretical knowledge and overall clinical capabilities, and significantly improve the teaching effect.

Purpose: To analyze the repair effect of glass fiber post combined with zirconia all ceramic crown in tooth defects and explore the relevant factors affecting the therapeutic effect.

Methods: A total of 100 patients(142 teeth) with dental defects admitted to Shandong Provincial Chronic Disease Hospital from January 2018 to January 2021 were selected as the study subjects. They were divided into an experimental group (glass fiber post combined with zirconia all ceramic crown restoration) with 70 patients(98 teeth) and the control group (metal post combined with zirconium dioxide all ceramic crown) with 30 patients (44 teeth). Clinical follow-up was conducted 6 months after surgery to compare the differences in dental restoration effectiveness, gingival bleeding index (BI), plaque index (PLI), and probing depth (PD) between the two groups of patients. According to the 6-month postoperative repair effect of the experimental group, patients were divided into two groups: poor repair group and good repair group. Single factor and multi-factor analysis was used to analyze the factors affecting the repair effect of glass fiber post combined with zirconia all ceramic crown. Statistical analysis was performed with SPSS 22.0 software package.

Results: Six months after surgery, the proportion of grade A, grade B and grade C in the experimental group was 76.5%, 20.4% and 3.1%, respectively, while the control group was 50.0%, 40.0%, and 10.0%. The dental restoration effect in the experimental group was significantly better than that in the control group (P=0.008). Before surgery, there was no significant difference in BI, PLI and PD between the two groups of patients (P＞0.05). Six months after treatment, the BI, PLI and PD in the experimental group were significantly lower than those in the control group(P＜0.05). Compared with the group with good repair, the group with poor repair had a higher proportion of smoking and stepped broken end morphology (P＜0.05). Using smoking (yes=1, no=0) and broken end shape (step shape=1, blade shape=0) as independent variables, and efficacy (poor repair group=1, good repair group=0) as dependent variables, logistic regression analysis showed the factors that smoking and broken end shape affected the treatment efficacy(P＜0.05).

Conclusions: Compared with metal post zirconium dioxide all ceramic crown restoration, glass fiber post combined with zirconium dioxide all ceramic crown restoration is more effective, but its restoration effect is affected by smoking and the shape of the broken end. Clinical intervention measures should be taken to improve the restoration effect.

Purpose: To study the correlation between the levels of inflammatory factors in gingival crevicular fluid and implant stability and prognosis after oral implant restoration.

Methods: A total of 78 patients who underwent oral implant restoration surgery from August 2022 to August 2023 were selected as the experimental group. According to the results of X-ray examination 1 month after implantation, the patients were divided into poor prognosis subgroup(n=33) and good prognosis subgroup(n=45), another 60 healthy subjects who underwent physical examination during the same period were selected as the control group. The gingival crevicular fluid of all research subjects was collected, and chemiluminescent immunoassay was used to determine high-sensitivity C-reactive protein (hsCRP), and enzyme-linked immunosorbent assay was used to determine amyloid A(SAA) and procalcitonin (PCT), implant stability quotient(ISQ) was recorded after surgery. Pearson's correlation analysis was used to evaluate the correlation between inflammatory factors in gingival crevicular fluid and ISQ, and multifactor logistic regression analysis was used to analyze the relationship between inflammatory factors in gingival crevicular fluid and implant prognosis. Statistical analysis was performed with SPSS 22.0 software package.

Results: The levels of hsCRP, SAA and PCT in the gingival crevicular fluid of the experimental group were significantly higher than the control group(P＜0.05), the levels of hsCRP, SAA and PCT in the gingival crevicular fluid of the poor prognosis subgroup were significantly higher than the good prognosis subgroup (P＜0.05). The initial and second-stage ISQ values of the patients in the experimental group were (74.08±6.12) and (76.78±5.11) respectively. Pearson's correlation analysis results showed that the gingival crevicular fluid hsCRP, SAA and PCT were significantly negatively correlated with initial and second-stage ISQ(P＜0.05); multi-factor logistic regression analysis results showed that hsCRP, SAA and PCT in gingival crevicular fluid were important factors affecting the prognosis of patients with dental implants(P＜0.05).

Conclusions: The levels of hsCRP, SAA and PCT in the gingival crevicular fluid after oral implant restoration are significantly increased, which are related to the stability and prognosis of the implant.

Purpose: To evaluate the preclinical teaching effects of digital virtual simulation system applied in window-type veneer tooth preparation of undergraduate dental students.

Methods: In the early clinical exposure of five-year undergraduate students from School of Stomatology, Fudan University, Simodont virtual simulation system and conventional phantom-simulator system were alternatively used in the preclinical teaching of veneer tooth preparation. First, after theoretical training and tests of veneer tooth preparation, twenty-three students were randomly divided into two groups. Students in group of virtual simulation priority were trained using Simodont virtual simulation system, while those in group of phantom priority were trained using traditional phantom head to complete the window-type veneer tooth preparation within the specified time, followed by theoretical test again for all students. Then, the two groups of students exchanged training systems and completed the window-type veneer tooth preparation and assessment in the same time. All students were asked to fill up a teaching questionnaire for the evaluation of two training methods. The data were collected and analyzed using SPSS 21.0 software package.

Results: The scores of veneer preparations in labial surface cutting volume, labial surface form, cervical margin design, proximal margin design using traditional phantom head training system were significantly higher than those using Simodont virtual simulation system(P＜0.05). Statistical results showed that the scores of labial surface cutting volume of phantom priority group were significantly higher than those of virtual simulation priority group(P＜0.05), while the scores of incisal length of virtual simulation priority group were significantly higher than those of phantom priority group(P＜0.05). The results of the questionnaire showed that the majority of students believed that the traditional phantom head was more helpful than Simodont virtual simulation in identification of labial surface cutting volume, margin form, depth of indicator groove, cutting force, while Simodont virtual simulation was superior to traditional phantom head in safety and future development tendency.

Conclusions: It is necessary to give full consideration of the advantages and limitations of digital virtual simulation system and design reasonable teaching plans. Combined application of digital virtual simulation system and traditional phantom head should be used to improve the preclinical teaching effects of veneer tooth preparation skills for undergraduate dental students.