Three-Dimensional Bio-Printed Tubular Tissue Using Dermal Fibroblast Cells as a New Tissue-Engineered Vascular Graft for Venous Replacement.

IF 3.1 3区 医学 Q2 ENGINEERING, BIOMEDICAL ASAIO Journal Pub Date : 2024-11-01 Epub Date: 2024-05-03 DOI:10.1097/MAT.0000000000002224
Makoto Hayasaka, Takashi Kokudo, Junichi Kaneko, Takehiro Chiyoda, Anna Nakamura, Manabu Itoh, Kazuhiro Endo, Koichi Nakayama, Kiyoshi Hasegawa
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Abstract

The current study was a preliminary evaluation of the feasibility and biologic features of three-dimensionally bio-printed tissue-engineered (3D bio-printed) vascular grafts comprising dermal fibroblast spheroids for venous replacement in rats and swine. The scaffold-free tubular tissue was made by the 3D bio-printer with normal human dermal fibroblasts. The tubular tissues were implanted into the infrarenal inferior vena cava of 4 male F344-rnu/rnu athymic nude rats and the short-term patency and histologic features were analyzed. A larger 3D bio-printed swine dermal fibroblast-derived prototype of tubular tissue was implanted into the right jugular vein of a swine and patency was evaluated at 4 weeks. The short-term patency rate was 100%. Immunohistochemistry analysis showed von Willebrand factor positivity on day 2, with more limited positivity observed on the luminal surface on day 5. Although the cross-sectional area of the wall differed significantly between preimplantation and days 2 and 5, suggesting swelling of the tubular tissue wall (both p < 0.01), the luminal diameter of the tubular tissues was not significantly altered during this period. The 3D bio-printed scaffold-free tubular tissues using human dermal or swine fibroblast spheroids may produce better tissue-engineered vascular grafts for venous replacement in rats or swine.

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利用真皮成纤维细胞的三维生物打印管状组织作为用于静脉置换的新型组织工程血管移植物
目前的研究是初步评估由真皮成纤维细胞球体组成的三维生物打印组织工程(3D 生物打印)血管移植物用于大鼠和猪静脉置换的可行性和生物特征。无支架管状组织是由三维生物打印机用正常人的真皮成纤维细胞制成的。将管状组织植入 4 只雄性 F344-rnu/rnu 无胸腺裸鼠的肾下腔静脉,分析其短期通畅性和组织学特征。将一个更大的三维生物打印猪真皮成纤维细胞衍生的管状组织原型植入一头猪的右颈静脉,并在 4 周后评估其通畅性。短期通畅率为 100%。免疫组化分析表明,第 2 天冯-威廉因子呈阳性,第 5 天在管腔表面观察到更有限的阳性。虽然管壁的横截面积在植入前与第 2 天和第 5 天之间存在显著差异,表明管壁组织发生了肿胀(均 p < 0.01),但在此期间,管腔组织的直径并没有发生显著变化。利用人体真皮或猪成纤维细胞球体进行三维生物打印的无支架小管组织可能会产生更好的组织工程血管移植物,用于大鼠或猪的静脉置换。
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来源期刊
ASAIO Journal
ASAIO Journal 医学-工程:生物医学
CiteScore
6.60
自引率
7.10%
发文量
651
审稿时长
4-8 weeks
期刊介绍: ASAIO Journal is in the forefront of artificial organ research and development. On the cutting edge of innovative technology, it features peer-reviewed articles of the highest quality that describe research, development, the most recent advances in the design of artificial organ devices and findings from initial testing. Bimonthly, the ASAIO Journal features state-of-the-art investigations, laboratory and clinical trials, and discussions and opinions from experts around the world. The official publication of the American Society for Artificial Internal Organs.
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