Impact of breath sample collection method and length of storage of breath samples in Tedlar bags on the level of selected volatiles assessed using gas chromatography-ion mobility spectrometry (GC-IMS).

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of breath research Pub Date : 2024-05-17 DOI:10.1088/1752-7163/ad4736
Barbora Czippelová, Slavomíra Nováková, Miroslava Šarlinová, Eva Baranovičová, Anna Urbanová, Zuzana Turianiková, Jana Čerňanová Krohová, Erika Halašová, Henrieta Škovierová
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Abstract

The analysis of volatile organic compounds (VOCs) in exhaled air has attracted the interest of the scientific community because it provides the possibility of monitoring physiological and metabolic processes and non-invasive diagnostics of various diseases. However, this method remains underused in clinical practice as well as in research because of the lack of standardized procedures for the collection, storage and transport of breath samples, which would guarantee good reproducibility and comparability of results. The method of sampling, as well as the storage time of the breath samples in the polymer bags used for sample storage and transport, affect the composition and concentration of VOCs present in the breath samples. The aim of our study was to compare breath samples obtained using two methods with fully disposable equipment: a Haldane sampling tube intended for direct breath collection and breath samples exhaled into a transparent Tedlar bag. The second task was to monitor the stability of selected compounds of real breath samples stored in a Tedlar bag for 6 h. Gas chromatography coupled with ion mobility spectrometry (GC-IMS) implemented in the BreathSpec®device was used to analyse exhaled breath. Our results showed a significant difference in the signal intensity of some volatiles when taking a breath sample with a Haldane tube and a Tedlar bag. Due to its endogenous origin, acetone levels were significantly higher when the Haldane tube sampler was used while elevated levels of 2-propanol and unidentified VOC (designated as VOC 3) in the Tedlar bag samples likely originated from contamination of the Tedlar bags. The VOC stability study revealed compound-specific signal intensity changes of the selected VOCs with storage time in the Tedlar bags, with some volatiles showing increasing signal intensity during storage in Tedlar bags. This limits the use of Tedlar bags only for very limited time and carefully selected purpose. Our results highlight the importance of careful design and implementation of experiments and clinical protocols to obtain relevant and reliable results.

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使用气相色谱-离子迁移谱法(GC-IMS)评估呼气样本收集方法和在 Tedlar 袋中储存呼气样本的时间对选定挥发性物质含量的影响。
呼出空气中挥发性有机化合物 (VOC) 的分析引起了科学界的兴趣,因为它提供了监测生理和代谢过程以及对各种疾病进行无创诊断的可能性。然而,由于缺乏收集、储存和运输呼气样本的标准化程序,这种方法在临床实践和研究中仍未得到充分利用,无法保证结果的良好重现性和可比性。采样方法以及呼气样本在用于样本储存和运输的聚合物袋中的储存时间会影响呼气样本中挥发性有机化合物的成分和浓度。我们的研究旨在比较使用两种方法和完全一次性设备获取的呼气样本:直接采集呼气样本的霍尔丹采样管和呼入透明 Tedlar 袋的呼气样本。第二项任务是监测在 Tedlar 袋中储存 6 小时的真实呼气样本中特定化合物的稳定性。BreathSpec® 设备采用气相色谱-离子迁移谱法(GC-IMS)分析呼出的气体。结果表明,使用霍尔丹管和 Tedlar 袋采集呼气样本时,某些挥发性物质的信号强度存在明显差异。使用 Haldane 管采样器时,丙酮的含量明显较高,而使用 Tedlar 袋采样时,2-丙醇和不明挥发性有机化合物的含量较高,这可能是由于 Tedlar 袋受到了污染。挥发性有机化合物稳定性研究显示,所选挥发性有机化合物的特定信号强度会随着在 Tedlar 袋中储存时间的延长而发生变化,某些挥发物在 Tedlar 袋储存期间信号强度会增加。我们的研究结果凸显了精心设计和实施实验和临床方案对获得相关可靠结果的重要性。
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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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