OXA-48-like carbapenemases in Proteus mirabilis - novel genetic environments and a challenge for detection.

IF 8.4 2区 医学 Q1 IMMUNOLOGY Emerging Microbes & Infections Pub Date : 2024-12-01 Epub Date: 2024-05-23 DOI:10.1080/22221751.2024.2353310
Janko Sattler, Janina Noster, Yvonne Stelzer, Martina Spille, Sina Schäfer, Kyriaki Xanthopoulou, Julian Sommer, Jonathan Jantsch, Silke Peter, Stephan Göttig, Sören G Gatermann, Axel Hamprecht
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Abstract

OXA-48-like enzymes represent the most frequently detected carbapenemases in Enterobacterales in Western Europe, North Africa and the Middle East. In contrast to other species, the presence of OXA-48-like in Proteus mirabilis leads to an unusually susceptible phenotype with low MICs for carbapenems and piperacillin-tazobactam, which is easily missed in the diagnostic laboratory. So far, there is little data available on the genetic environments of the corresponding genes, blaOXA-48-like, in P. mirabilis. In this study susceptibility phenotypes and genomic data of 13 OXA-48-like-producing P. mirabilis were investigated (OXA-48, n = 9; OXA-181, n = 3; OXA-162, n = 1). Ten isolates were susceptible to meropenem and ertapenem and three isolates were susceptible to piperacillin-tazobactam. The gene blaOXA-48 was chromosomally located in 7/9 isolates. Thereof, in three isolates blaOXA-48 was inserted into a P. mirabilis genomic island. Of the three isolates harbouring blaOXA-181 one was located on an IncX3 plasmid and two were located on a novel MOBF plasmid, pOXA-P12, within the new transposon Tn7713. In 5/6 isolates with plasmidic location of blaOXA-48-like, the plasmids could conjugate to E. coli recipients in vitro. Vice versa, blaOXA-48-carrying plasmids could conjugate from other Enterobacterales into a P. mirabilis recipient. These data show a high diversity of blaOXA-48-like genetic environments compared to other Enterobacterales, where genetic environments are quite homogenous. Given the difficult-to-detect phenotype of OXA-48-like-producing P. mirabilis and the location of blaOXA-48-like on mobile genetic elements, it is likely that OXA-48-like-producing P. mirabilis can disseminate, escape most surveillance systems, and contribute to a hidden spread of OXA-48-like.

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奇异变形杆菌中的 OXA-48 类碳青霉烯酶--新的遗传环境和检测挑战。
OXA-48 样酶是西欧、北非和中东肠杆菌中最常检测到的碳青霉烯酶。与其他物种不同的是,奇异变形杆菌中 OXA-48-like 酶的存在导致其表型异常易感,对碳青霉烯类和哌拉西林-他唑巴坦的 MIC 值较低,在诊断实验室中很容易被漏诊。迄今为止,有关奇异变形杆菌中相应基因(blaOXA-48-like)遗传环境的数据还很少。本研究调查了 13 个产 OXA-48-like 的奇异变形杆菌的药敏表型和基因组数据(OXA-48,n=9;OXA-181,n=3;OXA-162,n=1)。10 个分离株对美罗培南和厄他培南敏感,3 个分离株对哌拉西林-他唑巴坦敏感。7/9 个分离株的 blaOXA-48 基因位于染色体上。其中,3 个分离株的 blaOXA-48 被插入到 mirabilis 杆菌的基因组岛中。在携带 blaOXA-181 的 3 个分离物中,1 个位于 IncX3 质粒上,2 个位于新转座子 Tn7713 内的新型 MOBF 质粒 pOXA-P12 上。在 5/6 个分离株中,blaOXA-48-like 质粒可在体外与大肠杆菌受体结合。反之亦然,携带 blaOXA-48 的质粒也能从其他肠杆菌结合到奇异变形杆菌的受体中。这些数据表明,与遗传环境相当单一的其他肠杆菌相比,类似 blaOXA-48 的遗传环境具有很高的多样性。鉴于产生 OXA-48-like 的奇异变形杆菌的表型难以检测,而且 blaOXA-48-like 位于移动遗传因子上,因此产生 OXA-48-like 的奇异变形杆菌很可能会传播,逃过大多数监控系统的监控,并促成 OXA-48-like 的隐性传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Emerging Microbes & Infections
Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY
CiteScore
26.20
自引率
2.30%
发文量
276
审稿时长
20 weeks
期刊介绍: Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.
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