Dry Heat Sterilization of a Pelleted, Natural Ingredient Rodent Diet.

David M Kurtz, Tanya E Whiteside, Gordan Caviness, Fred B Lih
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Abstract

Sterilization of rodent feed is recommended to eliminate potential murine pathogens and minimize microbial variability between batches. Most research institutions sterilize feed using steam/pressure (autoclave) or irradiation. Both methods have advantages and disadvantages that contribute to their suitability, including cost, maintenance, availability, and alterations to the exposed product. Dry heat sterilization, which has been in use for over 75 y, uses higher temperatures and longer sterilization times than steam autoclave and is most often used for delicate instruments or products that would be damaged by water such as powders or oil-based liquids. Dry heat sterilization in vivaria has been limited to date but is gaining popularity due to lower initial purchase and ongoing operational costs as compared with steam autoclaves. Little published information exists on the effects of dry heat sterilization on animal feed. We evaluated the sterility and chemical alterations of a natural ingredient, pelleted, rodent diet (NIH-31) after exposure to dry heat. Feed sterility was achieved using a dry heat exposure temperature of 160 °C (320 °F) for 4 h. This exposure resulted in a significant loss of heat-labile vitamins and significantly more acrylamide production as compared with the nonsterile, irradiated, and autoclaved feed.

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天然成分啮齿动物颗粒食物的干热灭菌。
建议对啮齿动物饲料进行灭菌处理,以消除潜在的鼠类病原体,并将批次间的微生物变异性降至最低。大多数研究机构使用蒸汽/压力(高压灭菌器)或辐照对饲料进行灭菌。这两种方法各有优缺点,包括成本、维护、可用性和对暴露产品的改变等。干热灭菌法已使用超过 75 年,与蒸汽高压灭菌法相比,它使用的温度更高,灭菌时间更长,最常用于易碎器械或粉末或油性液体等会被水损坏的产品。迄今为止,干热灭菌在活体灭菌器中的应用还很有限,但由于与蒸汽高压灭菌器相比,干热灭菌器的初始购买成本和持续运行成本较低,因此越来越受到欢迎。有关干热灭菌对动物饲料影响的公开信息很少。我们评估了天然成分颗粒啮齿动物饲料(NIH-31)暴露于干热后的无菌性和化学变化。与未灭菌、辐照和高压灭菌的饲料相比,干热灭菌会导致热敏性维生素的大量损失和丙烯酰胺的产生。
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American Society of Laboratory Animal Practitioners Position Statement: Handling and Physical Restraint of Research Animals. American Society of Laboratory Animal Practitioners Position Statement: Definition of Animal Welfare. Effect of Novel High-fat Diet Feeding Methods on Food Wastage, Weight Gain, Hair Coat Grease Accumulation, and Scratching Behavior in C57BL/6NCrl Mice. Identification and Treatment of Fur Mites (Radfordia lemnina) in California Deer Mice (Peromyscus californicus) Using Selamectin. American Society of Laboratory Animal Practitioners Position Statement: Animal Care Principles.
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