Dry Heat Sterilization of a Pelleted, Natural Ingredient Rodent Diet.

David M Kurtz, Tanya E Whiteside, Gordan Caviness, Fred B Lih
{"title":"Dry Heat Sterilization of a Pelleted, Natural Ingredient Rodent Diet.","authors":"David M Kurtz, Tanya E Whiteside, Gordan Caviness, Fred B Lih","doi":"10.30802/AALAS-JAALAS-24-000005","DOIUrl":null,"url":null,"abstract":"<p><p>Sterilization of rodent feed is recommended to eliminate potential murine pathogens and minimize microbial variability between batches. Most research institutions sterilize feed using steam/pressure (autoclave) or irradiation. Both methods have advantages and disadvantages that contribute to their suitability, including cost, maintenance, availability, and alterations to the exposed product. Dry heat sterilization, which has been in use for over 75 y, uses higher temperatures and longer sterilization times than steam autoclave and is most often used for delicate instruments or products that would be damaged by water such as powders or oil-based liquids. Dry heat sterilization in vivaria has been limited to date but is gaining popularity due to lower initial purchase and ongoing operational costs as compared with steam autoclaves. Little published information exists on the effects of dry heat sterilization on animal feed. We evaluated the sterility and chemical alterations of a natural ingredient, pelleted, rodent diet (NIH-31) after exposure to dry heat. Feed sterility was achieved using a dry heat exposure temperature of 160 °C (320 °F) for 4 h. This exposure resulted in a significant loss of heat-labile vitamins and significantly more acrylamide production as compared with the nonsterile, irradiated, and autoclaved feed.</p>","PeriodicalId":94111,"journal":{"name":"Journal of the American Association for Laboratory Animal Science : JAALAS","volume":" ","pages":"377-384"},"PeriodicalIF":0.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11270041/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Association for Laboratory Animal Science : JAALAS","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30802/AALAS-JAALAS-24-000005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/29 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Sterilization of rodent feed is recommended to eliminate potential murine pathogens and minimize microbial variability between batches. Most research institutions sterilize feed using steam/pressure (autoclave) or irradiation. Both methods have advantages and disadvantages that contribute to their suitability, including cost, maintenance, availability, and alterations to the exposed product. Dry heat sterilization, which has been in use for over 75 y, uses higher temperatures and longer sterilization times than steam autoclave and is most often used for delicate instruments or products that would be damaged by water such as powders or oil-based liquids. Dry heat sterilization in vivaria has been limited to date but is gaining popularity due to lower initial purchase and ongoing operational costs as compared with steam autoclaves. Little published information exists on the effects of dry heat sterilization on animal feed. We evaluated the sterility and chemical alterations of a natural ingredient, pelleted, rodent diet (NIH-31) after exposure to dry heat. Feed sterility was achieved using a dry heat exposure temperature of 160 °C (320 °F) for 4 h. This exposure resulted in a significant loss of heat-labile vitamins and significantly more acrylamide production as compared with the nonsterile, irradiated, and autoclaved feed.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
天然成分啮齿动物颗粒食物的干热灭菌。
建议对啮齿动物饲料进行灭菌处理,以消除潜在的鼠类病原体,并将批次间的微生物变异性降至最低。大多数研究机构使用蒸汽/压力(高压灭菌器)或辐照对饲料进行灭菌。这两种方法各有优缺点,包括成本、维护、可用性和对暴露产品的改变等。干热灭菌法已使用超过 75 年,与蒸汽高压灭菌法相比,它使用的温度更高,灭菌时间更长,最常用于易碎器械或粉末或油性液体等会被水损坏的产品。迄今为止,干热灭菌在活体灭菌器中的应用还很有限,但由于与蒸汽高压灭菌器相比,干热灭菌器的初始购买成本和持续运行成本较低,因此越来越受到欢迎。有关干热灭菌对动物饲料影响的公开信息很少。我们评估了天然成分颗粒啮齿动物饲料(NIH-31)暴露于干热后的无菌性和化学变化。与未灭菌、辐照和高压灭菌的饲料相比,干热灭菌会导致热敏性维生素的大量损失和丙烯酰胺的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
American Society of Laboratory Animal Practitioners Position Statement: Handling and Physical Restraint of Research Animals. American Society of Laboratory Animal Practitioners Position Statement: Definition of Animal Welfare. Effect of Novel High-fat Diet Feeding Methods on Food Wastage, Weight Gain, Hair Coat Grease Accumulation, and Scratching Behavior in C57BL/6NCrl Mice. Identification and Treatment of Fur Mites (Radfordia lemnina) in California Deer Mice (Peromyscus californicus) Using Selamectin. American Society of Laboratory Animal Practitioners Position Statement: Animal Care Principles.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1