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American Society of Laboratory Animal Practitioners Position Statement: Handling and Physical Restraint of Research Animals. 美国实验动物从业者协会立场声明:研究动物的处理和物理约束。
Pub Date : 2024-09-20 DOI: 10.30802/AALAS-JAALAS-24-074
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引用次数: 0
Identification and Treatment of Fur Mites (Radfordia lemnina) in California Deer Mice (Peromyscus californicus) Using Selamectin. 使用塞拉米星鉴定和治疗加州鹿鼠(Peromyscus californicus)体内的毛螨(Radfordia lemnina)。
Pub Date : 2024-09-06 DOI: 10.30802/AALAS-JAALAS-24-055
Vittoria M Capria, Carrie L Freed

Peromyscus species have been used in research for decades, yet there are no specific reports of mite infestations in the laboratory setting despite many reports of various mite species found in wild Peromyscus. This study documents P. californicus infestation with Radfordia lemnina in an academic research setting. During the colony quarantine period, deer mice tested positive on a general mite PCR but negative on all species-specific mite PCR assays. Tape tests were performed on a subset of cages, and 21% were positive for adult mites or viable eggs. Mites were sent for sequencing and identified as R. lemnina, for which the natural host is Microtus pennsylvanicus. The entire colony was treated with selamectin, applied topically to the nape of the neck, and repeated one month later. All deer mice were successfully treated using a novel method of restraint, and no gross adverse reactions to selamectin treatment were noted. Tape tests were performed weekly to biweekly on a subset of deer mice, and PCR was used to confirm negative tape test results. PCR was positive at 14 wk posttreatment, and tape tests were intermittently positive for egg casings for 27 wk, indicating continued presence of genetic material but not necessarily an active infection. Weaned offspring were tape test and/or PCR negative at 12 and 21 wk posttreatment, providing further support for successful treatment. At 31 wk, 2 rounds of tape tests and PCR were both negative. This report documents a safe and effective treatment method for mites in P. californicus.

几十年来,啮齿类动物一直被用于研究,但尽管有许多关于在野生啮齿类动物中发现各种螨类的报告,却没有关于实验室环境中螨虫侵扰的具体报告。本研究记录了在学术研究环境中加利福尼亚啮齿类动物受到 Radfordia lemnina 侵染的情况。在群体检疫期间,鹿鼠的一般螨类 PCR 检测结果呈阳性,但所有物种特异性螨类 PCR 检测结果均呈阴性。对一部分笼子进行了胶带检测,21%的笼子中的成螨或存活卵呈阳性。螨虫被送去测序,确定为 R. lemnina,其自然宿主是 Microtus pennsylvanicus。用舍拉米丁对整个鹿群进行治疗,局部涂抹颈背,一个月后重复一次。所有鹿鼠都成功地使用了一种新的束缚方法,并且没有发现塞拉菌素治疗引起的严重不良反应。每周至每两周对部分鹿鼠进行一次胶带测试,并使用 PCR 来确认胶带测试的阴性结果。治疗后 14 周时 PCR 呈阳性,27 周内胶带检测卵壳间歇呈阳性,表明遗传物质继续存在,但不一定是活动感染。断奶后的后代在治疗后 12 和 21 周胶带检测和/或 PCR 检测均为阴性,进一步证明治疗成功。在 31 周时,两轮胶带测试和 PCR 均为阴性。本报告记录了一种安全有效的加州蛙螨虫治疗方法。
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引用次数: 0
American Society of Laboratory Animal Practitioners Position Statement: Definition of Animal Welfare. 美国实验动物从业者协会立场声明:动物福利的定义。
Pub Date : 2024-09-06 DOI: 10.30802/AALAS-JAALAS-24-071
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引用次数: 0
Effect of Novel High-fat Diet Feeding Methods on Food Wastage, Weight Gain, Hair Coat Grease Accumulation, and Scratching Behavior in C57BL/6NCrl Mice. 新型高脂饮食喂养法对 C57BL/6NCrl 小鼠食物浪费、体重增加、毛皮油脂积累和抓挠行为的影响
Pub Date : 2024-09-06 DOI: 10.30802/AALAS-JAALAS-24-059
Alyson R Guy, Molly Klores, Kevin Prestia, Mark Raymond, Skye Rasmussen

Soft-pelleted, high-fat diets (HFD) are greasy and crumble easily leading to food wastage and hair coat grease accumulation when mice are fed using commercially available feeders. The ideal HFD feeder design should reduce food wastage, facilitate mouse weight gain, and minimize variables such as hair coat grease accumulation that have the potential to alter scratching behaviors. Our study compared the feeding efficiency of 2 commercially available feeders (feeders A and E) to 4 novel feeder designs (feeders B, C, D, and F). Novel feeders had alterations in feeding aperture size, feeding surface area, feeder configuration, and level of food presentation. Male C57BL/6NCrl mice (n = 120; 4/cage) were randomly assigned to cages containing one of the 6 feeder types and were fed HFD for 12 wk. Feeders and cage bottoms were weighed before use and then weekly at the time of cage change. Mice were weighed before starting the HFD and then biweekly. Scratching behavior was video recorded at 0, 4, 8, and 12 wk. Hair coat grease accumulation was visually scored biweekly. Feeder A use was associated with the highest feed cost due to HFD wastage ($36.98 ± 1.54/cage/wk). Mice fed using Feeder A had the highest average weight gain (23.75 ± 0.8 g, P < 0.005). However, mice also had significantly higher hair coat grease accumulation scores (P < 0.05) and significantly increased scratching frequency at 4 wk (P < 0.05) when compared with mice fed using other feeder types. Novel feeder designs utilized 10 to 21 times less HFD dispensed when compared to feeder A. Mice fed using novel feeders also displayed improved welfare, as evidenced by low hair coat grease accumulation scores, and no significant differences in scratching frequency when compared with baseline behavior.

使用市售饲喂器喂养小鼠时,软颗粒高脂日粮(HFD)油腻且容易碎裂,导致食物浪费和毛皮油脂积累。理想的高脂日粮饲喂器设计应能减少食物浪费,促进小鼠体重增加,并最大限度地减少可能会改变小鼠抓挠行为的毛皮油脂积累等变量。我们的研究比较了两种市售喂食器(喂食器 A 和 E)和四种新型喂食器设计(喂食器 B、C、D 和 F)的喂食效率。新型喂食器在喂食孔径大小、喂食表面积、喂食器结构和食物展示程度方面都有所改变。雄性 C57BL/6NCrl 小鼠(n = 120;4 只/笼)被随机分配到装有 6 种喂食器之一的笼子中,并连续 12 周喂食高纤维食物。喂食器和笼底在使用前称重,然后每周换笼时称重。小鼠在开始喂食高氟日粮前称重,然后每两周称重一次。对小鼠在 0、4、8 和 12 周的抓挠行为进行录像记录。每两周目测一次毛皮油脂累积情况。由于高密度脂蛋白饲料的浪费,使用饲料 A 的饲料成本最高(36.98 ± 1.54 美元/笼/周)。使用饲料 A 喂养的小鼠平均增重最高(23.75 ± 0.8 克,P < 0.005)。然而,与使用其他类型喂料器喂养的小鼠相比,小鼠的毛被油脂累积评分也明显更高(P < 0.05),而且在4周时,小鼠的抓挠频率也明显增加(P < 0.05)。使用新型喂料器喂养的小鼠福利也有所改善,这表现在毛皮油脂累积得分较低,而且抓挠频率与基线行为相比没有显著差异。
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引用次数: 0
General Anesthesia Induced by a Combination of Medetomidine/Vatinoxan with Ketamine and Buprenorphine-ER in C57BL/6J Mice (Mus musculus). 在 C57BL/6J 小鼠(Mus musculus)中使用美托咪定/瓦替诺生与氯胺酮和丁丙诺啡-ER 的组合诱导全身麻醉。
Pub Date : 2024-09-05 DOI: 10.30802/AALAS-JAALAS-23-000120
Krystal Tien, Benjamin Franco, Eden D Alamaw, Katechan Jampachairsi, Kerriann Casey, Monika Huss, Cholawat Pacharinsak

Medetomidine/vatinoxan (Zenalpha®) is a novel anesthetic combination used as a sedative and analgesic in dogs. Vatinoxan minimizes adverse cardiopulmonary effects associated with medetomidine administration while preserving sedation and analgesia. In this study, we evaluated the clinical safety and efficacy of 3 dosage combinations of Zenalpha with ketamine and buprenorphine extended release (ER) as compared with xylazine with ketamine and buprenorphine-ER for anesthesia of C57BL/6J mice. We hypothesized that anesthesia with 0.5 mg/kg of Zenalpha would more reliably provide a surgical anesthetic plane, lower mortality, and fewer adverse physiologic effects as compared with anesthesia with 8 mg/kg of xylazine. Ten-week-old male and female C57BL/6J mice were randomly administered 1 of 4 anesthetic cocktails subcutaneously: ketamine (80 mg/kg) and buprenorphine-ER (0.5 mg/kg) with 1) xylazine (8 mg/kg; XKB); 2) Zenalpha (0.25 mg/kg; ZKB/0.25); 3) Zenalpha (0.5 mg/kg; ZKB/0.5); or 4) Zenalpha (1.0 mg/kg; ZKB/1.0). Following drug administration, we assessed the anesthesia induction time by measuring the time to loss of righting reflex and loss of paw withdrawal reflex (PWR). Upon reaching a loss of righting reflex, physiologic parameters including heart rate, respiratory rate, oxygen saturation, indirect mean arterial blood pressure, body temperature, jaw tone, and skin color were monitored every 5 min. Thirty minutes after anesthetic drug administration (TA), atipamezole (1 mg/kg SC) was administered. Recovery time was determined through time until return of PWR, righting reflex, and ambulation. Mice were monitored for 3 d postanesthesia. Results included: 1) ZKB anesthesia caused loss of PWR in a dose-dependent manner; 2) physiologic parameters were similar between XKB and ZKB mice by TA in 100% O₂; 3) ZKB groups took longer to recover and had a 20% to 30% mortality rate in the mid-to-high dosage groups. We conclude that anesthesia with 0.5 mg/kg of Zenalpha more reliably produced a surgical anesthetic plane but also led to decreased mean arterial pressure and increased mortality as compared with anesthesia with 8 mg/kg of xylazine. We recommend using Zenalpha (0.25 to 1.0 mg/kg) with 80 mg/kg ketamine and 0.5 mg/kg buprenorphine-ER to provide general anesthesia in C57BL/6 mice, along with supplemental 100% oxygen and atipamezole.

美托咪定/伐替诺生(Zenalpha®)是一种新型麻醉组合剂,可用作狗的镇静剂和镇痛剂。在保持镇静和镇痛效果的同时,华替诺生可最大程度地减少与美托咪定给药相关的心肺不良反应。在这项研究中,我们评估了泽纳尔法与氯胺酮和丁丙诺啡缓释剂(ER)的3种剂量组合与恶嗪与氯胺酮和丁丙诺啡缓释剂(ER)用于C57BL/6J小鼠麻醉的临床安全性和有效性比较。我们假设,与使用 8 毫克/千克的甲苯噻嗪进行麻醉相比,使用 0.5 毫克/千克的 Zenalpha 会更可靠地提供手术麻醉平面、更低的死亡率和更少的不良生理效应。对十周大的雄性和雌性 C57BL/6J 小鼠随机皮下注射四种混合麻醉剂中的一种:氯胺酮(80 毫克/千克)和丁丙诺啡-ER(0.5 毫克/千克)加 1) 异丙嗪(8 毫克/千克;XKB);2) Zenalpha(0.25 毫克/千克;ZKB/0.25);3) Zenalpha(0.5 毫克/千克;ZKB/0.5);或 4) Zenalpha(1.0 毫克/千克;ZKB/1.0)。给药后,我们通过测量右反射和爪退缩反射(PWR)消失的时间来评估麻醉诱导时间。在右反射消失后,每隔 5 分钟监测一次生理参数,包括心率、呼吸频率、血氧饱和度、间接平均动脉血压、体温、下颌张力和皮肤颜色。注射麻醉药物(TA)30 分钟后,注射阿替帕米唑(1 毫克/千克,皮下注射)。恢复时间通过脉搏波速度、右侧反射和行走的恢复时间来确定。对小鼠进行麻醉后 3 天的监测。结果包括1)ZKB 麻醉以剂量依赖的方式导致脉搏波速度减慢;2)在 100% O₂下,XKB 和 ZKB 小鼠的生理参数相似;3)ZKB 组的恢复时间更长,中高剂量组的死亡率为 20% 至 30%。我们的结论是,与使用 8 毫克/千克的甲苯噻嗪麻醉相比,使用 0.5 毫克/千克的 Zenalpha 麻醉能更可靠地产生手术麻醉平面,但也会导致平均动脉压下降和死亡率上升。我们建议使用 Zenalpha(0.25 至 1.0 毫克/千克)、80 毫克/千克氯胺酮和 0.5 毫克/千克丁丙诺啡-ER 对 C57BL/6 小鼠进行全身麻醉,同时补充 100% 氧气和阿替巴唑。
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引用次数: 0
American Society of Laboratory Animal Practitioners Position Statement: Animal Care Principles. 美国实验动物从业者协会立场声明:动物护理原则。
Pub Date : 2024-09-05 DOI: 10.30802/AALAS-JAALAS-24-073
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引用次数: 0
American Society of Laboratory Animal Practitioners Position Statement: Humane Endpoints. 美国实验动物从业者协会立场声明:人道终点。
Pub Date : 2024-09-05 DOI: 10.30802/AALAS-JAALAS-24-072
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引用次数: 0
The ARRIVE 2.0 Guidelines: Importance and Full Adoption by AALAS Journals. ARRIVE 2.0 指南:AALAS 期刊的重要性和全面采用。
Pub Date : 2024-09-05 DOI: 10.30802/AALAS-JAALAS-24-083
Mark A Suckow, Michael T Fallon
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引用次数: 0
Point-of-Care Cardiac Troponin I Assay Evaluation in New Zealand White Rabbits (Oryctolagus cuniculus). 新西兰白兔(Oryctolagus cuniculus)心肌肌钙蛋白 I 护理点检测评估。
Pub Date : 2024-09-04 DOI: 10.30802/AALAS-JAALAS-24-041
Sarah M Ozawa, Kursten V Pierce, Andrea Thomson, Nina A Moiseiwitsch, Jazz Stephens, Jessie LeGrand, Ashley C Brown, Hiroyuki Mochizuki

Cardiac troponin I (cTnI) is a cardiac-specific biomarker, used for the detection of myocardial injury. While rabbits develop naturally occurring cardiovascular disease, they are also an animal model for human disease; thus, rapid detection of cTnI has implications for both veterinary and human medicine. The objective of this study was to validate and establish a reference interval for a point-of-care (POC) cTnI assay in New Zealand White rabbits. In the first portion of the study, rabbit cardiac and skeletal muscle tissues were used to create homogenates, serially diluted with saline or rabbit whole blood, and run by repeated analysis on the POC assay. In the second portion of the study, a reference interval of peripheral whole blood cTnI was determined by robust methods from 49 New Zealand White rabbits. The least diluted cardiac muscle homogenates produced detectable cTnI (mean 23.12 ± 3.557 ng/mL), while skeletal muscle homogenates produced low to undetectable cTnI. The CV ranged from 0.00% to 32.51% for cTnI of diluted cardiac muscle homogenates. Rabbit cardiac homogenate diluted in blood had a linear relationship to cTnI concentration (Y = 0.2254 × X + 0.5396, R² = 0.975). The reference interval for cTnI in this population was less than 0.04 ng/mL. This POC assay may be useful when rapid detection of cTnI is needed and differentiation between normal and elevated values is required. Given the high CV, this assay may not be appropriate for cases that require high sensitivity or detection of low concentrations of cTnI.

心肌肌钙蛋白 I(cTnI)是一种心脏特异性生物标记物,用于检测心肌损伤。兔子会罹患自然发生的心血管疾病,同时也是人类疾病的动物模型;因此,快速检测 cTnI 对兽医和人类医学都有意义。本研究的目的是在新西兰白兔中验证并建立一种床旁 (POC) cTnI 检测方法的参考区间。在研究的第一部分,使用兔子的心肌和骨骼肌组织制作匀浆,用生理盐水或兔子全血进行系列稀释,并在 POC 检测仪上进行重复分析。在研究的第二部分,采用可靠的方法测定了 49 只新西兰白兔外周全血 cTnI 的参考区间。稀释度最低的心肌匀浆可检测到 cTnI(平均值为 23.12 ± 3.557 ng/mL),而骨骼肌匀浆产生的 cTnI 低至检测不到。稀释的心肌匀浆中 cTnI 的 CV 值范围为 0.00% 至 32.51%。血液中稀释的兔心肌匀浆与 cTnI 浓度呈线性关系(Y = 0.2254 × X + 0.5396,R² = 0.975)。该人群中 cTnI 的参考区间小于 0.04 纳克/毫升。当需要快速检测 cTnI 并区分正常值和升高值时,这种 POC 检测方法可能很有用。鉴于 CV 值较高,该检测方法可能不适合需要高灵敏度或检测低浓度 cTnI 的病例。
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引用次数: 0
Comprehensive Husbandry Protocol for Corydoras Catfish and Many Other Amazonian Species. 科里多拉斯鲶鱼和许多其他亚马逊鱼种的综合饲养规程。
Pub Date : 2024-09-02 DOI: 10.30802/AALAS-JAALAS-24-039
Austin Chiang, Sullivan S S Haine, Rebecca Goldring, Arne Jungwirth, Munir Siddiqui, Gerald Wilkinson, Andrea Manica, Riva J Riley

A variety of fish species have proven instrumental in the investigation of evolution, behavior, ecology, and physiology, among many other fields. Many model systems (e.g., zebrafish, guppies, and three-spined sticklebacks) have been maintained by institutions and have had protocols written with respect to their husbandry. Here we present the protocols we have developed to maintain and breed a variety of Corydoras catfish species, which are native to the tropical Americas. Corydoras species are excellent systems for investigating behavior, ecology, and other topics, and our husbandry protocols would be suitable for nearly every species in the genus. In addition, these protocols are appropriate for a variety of softwater Amazonian species, and we present options for a variety of housing and husbandry conditions. On the whole, we suggest that, in a scientific laboratory setting, the use of remineralized reverse osmosis water is most appropriate and that in context, a single measure, total dissolved solids, can be used to monitor the water chemistry for water introduced to fish enclosures.

事实证明,各种鱼类在研究进化、行为学、生态学和生理学等许多领域都发挥了重要作用。许多模式系统(如斑马鱼、幌鱼和三刺鱼)都由相关机构维护,并制定了饲养规程。在此,我们将介绍我们为饲养和繁殖原产于美洲热带地区的各种科氏鲶而制定的规程。鲯鳅是研究行为学、生态学和其他课题的绝佳系统,我们的饲养规程几乎适用于该属的所有物种。此外,这些饲养方法也适用于多种亚马逊河软水鱼种,我们还提供了多种饲养方法和饲养条件。总的来说,我们建议,在科学实验室环境中,使用再矿化反渗透水是最合适的,而且在具体情况下,可以使用溶解性总固体这一单一指标来监测引入鱼类围栏的水的化学性质。
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引用次数: 0
期刊
Journal of the American Association for Laboratory Animal Science : JAALAS
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