Quality control for Adeno-associated viral vector production.

Neuromethods Pub Date : 2023-01-01 Epub Date: 2023-02-07 DOI:10.1007/978-1-0716-2918-5_5
Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Tillgren, Alanna Fava, Gabrielle Clouse, Marcella Patrick, Karen Guerin, Melina Fan
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引用次数: 0

Abstract

Adeno-associated viral vectors (AAV) are frequently used by neuroscientists to deliver tools, such as biosensors and optogenetic and chemogenetic actuators, in vivo. Despite its widespread use, AAV vector characterization and quality control can vary between labs and viral vector cores leading to variable results and irreproducibility. This protocol describes some of the characterization and quality control assays necessary to confirm an AAV vector's titer, genomic identity, serotype and purity.

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腺相关病毒载体生产的质量控制。
腺相关病毒载体(AAV)经常被神经科学家用于在体内传递生物传感器、光遗传和化学遗传致动器等工具。尽管 AAV 被广泛使用,但不同实验室和病毒载体中心对 AAV 载体的表征和质量控制可能会有所不同,从而导致结果不一和不可再现性。本规程介绍了确认 AAV 载体滴度、基因组特征、血清型和纯度所需的一些表征和质量控制检测方法。
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