Advances in common in vitro cellular models of pulmonary fibrosis

IF 3.2 4区 医学 Q3 CELL BIOLOGY Immunology & Cell Biology Pub Date : 2024-05-07 DOI:10.1111/imcb.12756
Die Li, Xinyue Zhang, Ziqiong Song, Shan Zhao, Yuan Huang, Weibin Qian, Xinrui Cai
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Abstract

The development of in vitro models is essential for a comprehensive understanding and investigation of pulmonary fibrosis (PF) at both cellular and molecular levels. This study presents a literature review and an analysis of various cellular models used in scientific studies, specifically focusing on their applications in elucidating the pathogenesis of PF. Our study highlights the importance of taking a comprehensive approach to studing PF, emphasizing the necessity of considering multiple cell types and organs and integrating diverse analytical perspectives. Notably, primary cells demonstrate remarkable cell growth characteristics and gene expression profiles; however, their limited availability, maintenance challenges, inability for continuous propagation and susceptibility to phenotypic changes over time significantly limit their utility in scientific investigation. By contrast, immortalized cell lines are easily accessible, cultured and continuously propagated, although they may have some phenotypic differences from primary cells. Furthermore, in vitro coculture models offer a more practical and precise method to explore complex interactions among cells, tissues and organs. Consequently, when developing models of PF, researchers should thoroughly assess the advantages, limitations and relevant mechanisms of different cell models to ensure their selection is consistent with the research objectives.

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肺纤维化常见体外细胞模型的研究进展。
开发体外模型对于从细胞和分子水平全面了解和研究肺纤维化(PF)至关重要。本研究对科学研究中使用的各种细胞模型进行了文献综述和分析,特别侧重于它们在阐明肺纤维化发病机制方面的应用。我们的研究强调了采取综合方法研究 PF 的重要性,强调了考虑多种细胞类型和器官以及整合不同分析视角的必要性。值得注意的是,原代细胞表现出显著的细胞生长特性和基因表达谱;然而,由于其可用性有限、维护困难、无法持续繁殖以及表型易随时间发生变化等原因,大大限制了其在科学研究中的应用。相比之下,永生化细胞系很容易获得、培养和持续繁殖,尽管它们可能与原代细胞有一些表型差异。此外,体外共培养模型为探索细胞、组织和器官之间复杂的相互作用提供了更实用、更精确的方法。因此,在开发 PF 模型时,研究人员应全面评估不同细胞模型的优势、局限性和相关机制,以确保其选择符合研究目标。
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来源期刊
Immunology & Cell Biology
Immunology & Cell Biology 医学-免疫学
CiteScore
7.50
自引率
2.50%
发文量
98
审稿时长
4-8 weeks
期刊介绍: The Australasian Society for Immunology Incorporated (ASI) was created by the amalgamation in 1991 of the Australian Society for Immunology, formed in 1970, and the New Zealand Society for Immunology, formed in 1975. The aim of the Society is to encourage and support the discipline of immunology in the Australasian region. It is a broadly based Society, embracing clinical and experimental, cellular and molecular immunology in humans and animals. The Society provides a network for the exchange of information and for collaboration within Australia, New Zealand and overseas. ASI members have been prominent in advancing biological and medical research worldwide. We seek to encourage the study of immunology in Australia and New Zealand and are active in introducing young scientists to the discipline.
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