Effect of quaternized chitosan magnetic nanoparticles carrying indocyanine green phototherapy on cervical cancer cells

IF 2.1 4区 材料科学 Q3 CHEMISTRY, MULTIDISCIPLINARY Journal of Nanoparticle Research Pub Date : 2024-05-02 DOI:10.1007/s11051-024-06003-5
Xiaohui Tang, Qian Zhang, Kadireya Aikelamu, Jingya Bai, Rong Ma, Mei Wang, Chao Liu
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Abstract

The objective of the study is to prepare quaternized chitosan magnetic nanoparticles (ICG@Fe3O4@QCS) loaded with indocyanine green and to investigate their properties. Fe3O4@QCS nanoparticles were prepared by a one-part precipitation method and evaluated for their structure, particle size, morphology, and magnetic responsiveness. ICG@Fe3O4@QCS nanoparticles were prepared by electrostatic adsorption to investigate the properties of ICG@Fe3O4@QCS nanoparticles, such as particle size, zeta potential, encapsulation rate, drug loading, stability, photo- and thermo-conversion efficiencies, degree of release, biosafety, and in vitro antitumor property. Fe3O4@QCS nanoparticles were successfully prepared in the form of short rods with particle sizes around 20–30 nm, positively charged, with basic Fe3O4 skeleton, and possessing specific magnetic responsiveness. ICG@Fe3O4@QCS nanoparticles were successfully prepared, particle size is around 100–150 nm, charged positively, the encapsulation rate is ≥ 90%, the drug loading is 0.05–2.00%, the release is slower compared with the ICG solution, and hemolysis rate is less than 2% in all cases; in the laser irradiation, the nanoparticles can produce ROS and thermal effects and have the function of NIR imaging. Antitumor experiments in vitro showed that ICG@Fe3O4@QCS nanoparticles had strong phototoxicity, had a particular killing effect on tumor cells, and could promote Hela cell apoptosis. ICG@Fe3O4@QCS nanoparticles can be successfully prepared by the electrostatic adsorption method, with the stable nature of ICG@Fe3O4@QCS nanoparticles, which can improve the bioavailability of ICG, and the prepared nanoparticles have good antitumor properties.

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携带吲哚菁绿光疗法的季铵化壳聚糖磁性纳米粒子对宫颈癌细胞的影响
本研究旨在制备负载有吲哚菁绿的季铵化壳聚糖磁性纳米粒子(ICG@Fe3O4@QCS),并研究其特性。研究采用单组分沉淀法制备了 Fe3O4@QCS 纳米粒子,并对其结构、粒度、形态和磁响应性进行了评估。采用静电吸附法制备了ICG@Fe3O4@QCS纳米颗粒,研究了ICG@Fe3O4@QCS纳米颗粒的粒径、ZETA电位、包封率、载药量、稳定性、光热转化效率、释放度、生物安全性和体外抗肿瘤性能等特性。成功制备的 Fe3O4@QCS 纳米粒子呈短棒状,粒径约 20-30 nm,带正电荷,以碱性 Fe3O4 为骨架,具有特定的磁响应性。成功制备了ICG@Fe3O4@QCS纳米颗粒,粒径约为100-150 nm,带正电荷,包封率≥90%,载药量为0.05-2.00%,释放速度较ICG溶液慢,溶血率均小于2%;在激光照射下,纳米颗粒可产生ROS和热效应,并具有近红外成像功能。体外抗肿瘤实验表明,ICG@Fe3O4@QCS 纳米粒子具有很强的光毒性,对肿瘤细胞有特殊的杀伤作用,并能促进 Hela 细胞凋亡。静电吸附法可成功制备ICG@Fe3O4@QCS纳米粒子,ICG@Fe3O4@QCS纳米粒子性质稳定,可提高ICG的生物利用度,制备的纳米粒子具有良好的抗肿瘤性能。
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来源期刊
Journal of Nanoparticle Research
Journal of Nanoparticle Research 工程技术-材料科学:综合
CiteScore
4.40
自引率
4.00%
发文量
198
审稿时长
3.9 months
期刊介绍: The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size. Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology. The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.
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