Cytotoxicity effects and differentiation potential of ormocer-based and nanohybrid composite resins on human dental pulp stem cells

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Dental Materials Pub Date : 2024-06-01 DOI:10.1016/j.dental.2024.05.011

C. Del Giudice , C. Rengo , M. Maglitto , N.G. Armogida , F. Iaculli , S. Rengo , C. Menale , G. Spagnuolo

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引用次数: 0

Abstract

Objective

to compare conventional nanohybrid (Ceram.x Spectra) and ormocer-based (Admira fusion) dental composite resins effects on human dental pulp stem cells (hDPSCs) in terms of cytotoxicity, self-renewal, migration and osteogenic differentiation.

Methods

hDPSCs were cultured in presence of different dilutions (undiluted, form 1:2 to 1:100) of CeramX (CX) and Admira fusion (AD) eluates and viability assay in standard or osteogenic conditions were performed. Samples and eluates were prepared according to ISO 10993–12. In addition, apoptosis, self-renewal and migration activity evaluations were carried out. Osteogenic differentiation potential was tested by Alkaline Phosphatase Activity, alizarin red staining and gene expression of specific markers (ALP, RUNX2, OCN, OPN and COL1α1). Statistical analysis was performed by means of a One-way analysis of variance (One-way ANOVA) followed by a Tukey’s test for multiple comparison; results were presented as mean ± standard error of mean (SEM).

Results

Admira Fusion demonstrated to be highly biocompatible and showed positive effects on hDPSCs proliferation and differentiation; on the contrary, conventional nanohybrid composite showed to be more cytotoxic and without any notable effect on stem cells differentiation. Moreover, the obtained results were further corroborated by a significant upregulation of osteogenic differentiation markers obtained in presence of ormocer-based composite resin eluate. Specifically, in AD 1:50 group expression levels of ALP, Runx2, Col1α1 were double than control (ALP, p = 0.045; Runx2, p = 0.003; Col1α1, p = 0.001) and CX 1:50 (ALP, p = 0.006; RUNX2, p = 0.029; Col1α1, p = 0.005). Moreover, in the same group, OPN and OCN resulted about 5 times more expressed as compared to control (OPN, p = 0.009; OCN, p = 0.0005) and CX 1:50 (OPN, p = 0.012; OCN, p = 0.0006).

Significance

The less cytotoxicity obtained by AD than conventional nanohybrid composite may be attributed to a reduced monomers release in the oral environment, supporting the hypothesis of limited adverse effect and enhanced healing potential, mainly when the material is positioned in close contact with pulp tissue.

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ormocer基和纳米杂化复合树脂对人牙髓干细胞的细胞毒性效应和分化潜能

目的：比较传统纳米杂化（Ceram.x Spectra）和基于ormocer（Admira fusion）的牙科复合树脂在细胞毒性、自我更新、迁移和成骨分化方面对人牙髓干细胞（hDPSCs）的影响。方法：在不同稀释度（未稀释，1:2 至 1:100）的 CeramX（CX）和 Admira fusion（AD）洗脱液存在下培养 hDPSCs，并在标准或成骨条件下进行活力检测。样品和洗脱液按照 ISO 10993-12 标准制备。此外，还进行了细胞凋亡、自我更新和迁移活性评估。碱性磷酸酶活性、茜素红染色和特定标记物（ALP、RUNX2、OCN、OPN 和 COL1α1）的基因表达检测了成骨分化潜能。统计分析采用单因素方差分析（One-way ANOVA），然后进行Tukey多重比较检验；结果以平均值±平均值标准误差（SEM）表示：结果表明，Admira Fusion 具有很高的生物相容性，对 hDPSCs 的增殖和分化有积极作用；相反，传统的纳米杂化复合材料细胞毒性较强，对干细胞分化没有明显影响。此外，在ormocer基复合树脂洗脱液的存在下，成骨分化标志物显著上调，进一步证实了上述结果。具体来说，在 AD 1:50 组中，ALP、Runx2、Col1α1 的表达水平是对照组（ALP，p = 0.045；Runx2，p = 0.003；Col1α1，p = 0.001）和 CX 1:50 组（ALP，p = 0.006；RUNX2，p = 0.029；Col1α1，p = 0.005）的两倍。此外，与对照组（OPN，p = 0.009；OCN，p = 0.0005）和 CX 1:50 组（OPN，p = 0.012；OCN，p = 0.0006）相比，同一组中 OPN 和 OCN 的表达量增加了约 5 倍：AD比传统纳米杂化复合材料的细胞毒性更小，这可能是由于单体在口腔环境中的释放量减少，支持了不良影响有限和愈合潜力增强的假设，主要是在材料与牙髓组织紧密接触的情况下。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.