Identification of Rmg11 in Tetraploid Wheat as a New Blast Resistance Gene with Tolerance to High Temperature.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES Phytopathology Pub Date : 2024-08-01 Epub Date: 2024-08-13 DOI:10.1094/PHYTO-02-24-0074-R

M Thoihidul Islam, Chika Nago, Motohiro Yoshioka, Trinh Thi Phuong Vy, Yukio Tosa, Soichiro Asuke

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Abstract

Wheat blast caused by Pyricularia oryzae pathotype Triticum has spread to Asia (Bangladesh) and Africa (Zambia) from the endemic region of South America. Wheat varieties with durable resistance are needed, but very limited resistance resources are currently available. After screening tetraploid wheat accessions, we found an exceptional accession St19 (Triticum dicoccum, KU-114). Primary leaves of St19 were resistant not only to Brazilian isolate Br48 (a carrier of Type eI of AVR-Rmg8) but also to Br48ΔA8, an AVR-Rmg8 disruptant of Br48, even at 30°C, suggesting that the resistance of St19 is tolerant to high temperature and controlled by a gene or genes other than Rmg8. When an F₂ population derived from a cross between St19 and St30 (a susceptible accession of T. paleocolchicum, KU-191) was inoculated with Br48, resistant and susceptible seedlings segregated in a 3:1 ratio, indicating that resistance of St19 is conferred by a single gene. We designated this gene Rmg11. Molecular mapping revealed that the RMG11 locus is located on the short arm of chromosome 7A. Rmg11 is effective not only against other two Brazilian isolates (Br5 and Br116.5) but also against Bangladeshi isolates (T-108 and T-109) at the seedling stage. At the heading stage, lines containing Rmg11 were highly susceptible to the Bangladeshi isolates but moderately resistant to the Brazilian isolates. Stacking of Rmg11 with Rmg8 and the 2NS segment is highly recommended to achieve durable wheat blast resistance.

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鉴定四倍体小麦中的 Rmg11，作为一种耐高温的新抗瘟基因。

由 Pyricularia oryzae 病原型 Triticum 引起的小麦瘟疫已从南美洲流行区蔓延到亚洲（孟加拉国）和非洲（赞比亚）。需要具有持久抗性的小麦品种，但目前可用的抗性资源非常有限。在对四倍体小麦品种进行筛选后，我们发现了一个特殊的品种 St19（Triticum dicoccum，KU-114）。St19 的初生叶片不仅对巴西分离株 Br48（AVR-Rmg8 eI 型的携带者）具有抗性，而且对 Br48 的 AVR-Rmg8 干扰株 Br48ΔA8 也具有抗性，即使在 30℃的条件下也是如此。将 St19 与 St30（古小结球甘蓝的易感品种，KU-191）杂交产生的 F2 群体接种 Br48，抗性幼苗与易感幼苗的分离比例为 3:1，表明 St19 的抗性是由单一基因赋予的。我们将该基因命名为 Rmg11。分子图谱显示，RMG11 基因座位于染色体 7A 的短臂上。Rmg11 不仅对其他两种巴西分离株（Br5 和 Br116.5）有效，而且在苗期对孟加拉国分离株（T-108 和 T-109）也有效。在头花期，含有 Rmg11 的品系对孟加拉国分离物高度敏感，但对巴西分离物有中等抗性。强烈建议将 Rmg11 与 Rmg8 和 2NS 片段堆叠，以获得持久的小麦稻瘟病抗性。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.