Multi-omics explores the potential regulatory role of acetylation modification in flavonoid biosynthesis of Ginkgo biloba.

IF 3.5 2区 农林科学 Q1 FORESTRY Tree physiology Pub Date : 2024-06-03 DOI:10.1093/treephys/tpae051
Xiaomeng Liu, Jiabao Ye, Xiaoxi Zhang, Ke Yang, Jiarui Zheng, Shuiyuan Cheng, Weiwei Zhang, Feng Xu
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Abstract

Flavonoids are crucial medicinal active ingredients in Ginkgo biloba L. However, the effect of protein post-translational modifications on flavonoid biosynthesis remains poorly explored. Lysine acetylation, a reversible post-translational modification, plays a crucial role in metabolic regulation. This study aims to investigate the potential role of acetylation in G. biloba flavonoid biosynthesis. Through comprehensive analysis of transcriptomes, metabolomes, proteomes and acetylated proteins in different tissues, a total of 11,788 lysine acetylation sites were identified on 4324 acetylated proteins, including 89 acetylation sites on 23 proteins. Additionally, 128 types of differentially accumulated flavonoids were identified among tissues, and a dataset of differentially expressed genes related to the flavonoid biosynthesis pathway was constructed. Twelve (CHI, C3H1, ANR, DFR, CCoAOMT1, F3H1, F3H2, CCoAOMT2, C3H2, HCT, F3'5'H and FG2) acetylated proteins that might be involved in flavonoid biosynthesis were identified. Specifically, we found that the modification levels of CCoAOMT1 and F3'5'H sites correlated with the catalytic production of homoeriodictyol and dihydromyricetin, respectively. Inhibitors of lysine deacetylase (trichostatin A) impacted total flavonoid content in different tissues and increased flavonoid levels in G. biloba roots. Treatment with trichostatin A revealed that expression levels of GbF3'5'H and GbCCoAOMT1 in stems and leaves aligned with total flavonoid content variations, while in roots, expression levels of GbC3H2 and GbFG2 corresponded to total flavonoid content changes. Collectively, these findings reveal for the first time the important role of acetylation in flavonoid biosynthesis.

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多组学探索乙酰化修饰在银杏叶类黄酮生物合成中的潜在调控作用。
类黄酮是银杏叶中重要的药用活性成分。然而,人们对蛋白质翻译后修饰(PTM)对类黄酮生物合成的影响仍然知之甚少。赖氨酸乙酰化是一种可逆的 PTM,在新陈代谢调节中起着至关重要的作用。本研究旨在探讨乙酰化在双叶黄酮生物合成中的潜在作用。通过对不同组织的转录组、代谢组、蛋白质组和乙酰化蛋白质进行全面分析,在 4324 个乙酰化蛋白质上共鉴定出 11788 个赖氨酸乙酰化位点,其中包括 23 个蛋白质上的 89 个乙酰化位点。此外,还鉴定了128种组织间不同积累的黄酮类化合物,并构建了与黄酮类化合物生物合成途径相关的差异表达基因数据集。鉴定出了12种(CHI、C3H1、ANR、DFR、CCoAOMT1、F3H1、F3H2、CCoAOMT2、C3H2、HCT、F3'5'H和FG2)可能参与类黄酮生物合成的乙酰化蛋白。具体而言,我们发现 CCoAOMT1 和 F3'5'H 位点的修饰水平分别与同源碘酪醇(homoeriodictyol)和二氢杨梅素(dihydromyetin)的催化生产相关。赖氨酸去乙酰化酶抑制剂(trichostatin A,TSA)会影响不同组织中的总黄酮含量,并增加双叶植物根中的黄酮含量。用TSA处理后发现,茎和叶中GbF3'5'H和GbCCoAOMT1的表达水平与总黄酮含量的变化一致,而在根中,GbC3H2和GbFG2的表达水平与总黄酮含量的变化一致。这些发现首次揭示了乙酰化在黄酮类化合物生物合成中的重要作用。
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来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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