SEM and LM insights: Spore morphology and its taxonomic significance in Sino Himalayan, Malesian, and European elements of Pteridaceae

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY Microscopy Research and Technique Pub Date : 2024-05-10 DOI:10.1002/jemt.24583

Syed Nasar Shah, Sezai Ercisli, Akhtar Zaman, Israr Ud Din, Fahim Ullah Khan, Muhammad Nauman Khan, Majid Iqbal, Baber Ali, Mohammad K. Okla

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Abstract

The Pteridaceae family, known for its taxonomic complexity, presents challenges in identification due to high variability among its species. This study investigates the spore morphology employing both SEM and LM techniques in 10 Pteridaceae taxa phytogeographicaly Sino-Himalayan, Malesian, and European elements in Pakistan. The taxa include Adiantum capillus-veneris, A. incisum, A. venustum, Aleuritopteris bicolor, Oeosporangium nitidulum, O. pteridioides, Onychium cryptogrammoides, O. vermae, Pteris cretica, and P. vittata. The objective is to assess their taxonomic relevance and develop a spore-based taxonomic key. Findings indicate differences in spore shape, sizes, exospore thickness, and in surface ornamentation highlighting the potential for taxonomic differentiation. Spores are trilete, and notable differences are observed in the dimension of spores in both distal and proximal sides. Equatorial dimensions vary between 35 and 50 μm, while the polar diameter ranges from 29 to 50 μm. SEM revealed different spore ornamentation types that show several useful characteristics establishing valuable taxonomic variations. The studied Adiantum taxa feature a perispore with tubercules and a micro-granulose surface. The spores of examined Oeosporangium and Aleuritopteris taxa shows cristate sculptures with variable ornamentations. Both species of Onychium have tuberculate-pleated tubercles with sinuous folds on both distal and proximal sides. The surface ornamentation among examined Pteris taxa show variability. PCA analysis indicated that spore quantitative data identified distinct groups, underscoring taxonomic significance. Nevertheless, there was variation observed in surface ornamentation and spore shape, indicating the potential for discrimination among taxa.

Research Highlights

Spore morphology of 10 Pteridaceae taxa has been investigated through LM and SEM.
Investigated species shows differences in spore shape, sizes, exospore thickness, and in surface ornamentation.
Ornamentation on the perispore provides several valuable characteristics, establishing useful taxonomic distinctions.
Spore morphological analysis is effective at the generic level, with minor distinctions discernible at the species level.

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SEM 和 LM 见解：中喜马拉雅、马来和欧洲翼手目植物的孢子形态及其分类意义。

翼手目科以其分类复杂而著称，由于其物种之间的高度变异性，给鉴定工作带来了挑战。本研究采用 SEM 和 LM 技术，对巴基斯坦境内 10 个具有中国-喜马拉雅、马来和欧洲植物地理特征的翼手目分类群的孢子形态进行了研究。这些分类群包括 Adiantum capillus-veneris、A. incisum、A. venustum、Aleuritopteris bicolor、Oeosporangium nitidulum、O. pteridioides、Onychium cryptogrammoides、O. vermae、Pteris cretica 和 P. vittata。目的是评估它们在分类学上的相关性，并开发基于孢子的分类钥匙。研究结果表明，孢子的形状、大小、外孢子厚度和表面装饰都存在差异，这凸显了它们在分类学上的差异。孢子呈三裂状，远端和近端孢子的尺寸存在明显差异。赤道直径在 35 至 50 μm 之间，而两极直径在 29 至 50 μm 之间。扫描电子显微镜显示了不同的孢子装饰类型，这些类型显示了一些有用的特征，建立了有价值的分类变异。所研究的 Adiantum 类群的孢子具有小瘤和微颗粒表面。所研究的 Oeosporangium 和 Aleuritopteris 类群的孢子显示出具有不同装饰的皱缩雕刻。Onychium 的两个种都有具瘤褶的小瘤，小瘤的远端和近端都有蜿蜒的褶皱。所研究的翼手目类群之间的表面装饰也存在差异。PCA 分析表明，孢子定量数据确定了不同的类群，强调了分类的重要性。然而，在表面装饰和孢子形状方面也观察到了差异，这表明分类群之间有可能存在差异。研究亮点：通过 LM 和 SEM 对 10 个翼手目分类群的孢子形态进行了研究。所研究的物种在孢子形状、大小、外孢子厚度和表面装饰方面存在差异。孢子表面的装饰提供了一些有价值的特征，从而建立了有用的分类区分。孢子形态学分析在属的层面上是有效的，在种的层面上也能看出细微的差别。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.