Eccentric muscle-damaging exercise in the heat lowers cellular stress prior to and immediately following future exertional heat exposure

IF 3.3 3区生物学 Q3 CELL BIOLOGY Cell Stress & Chaperones Pub Date : 2024-05-10 DOI:10.1016/j.cstres.2024.05.001

Ryan A. Dunn , Hui-Ying Luk , Casey R. Appell , Nigel C. Jiwan , Marcos S. Keefe , Jan-Joseph S. Rolloque , Yasuki Sekiguchi

{"title":"Eccentric muscle-damaging exercise in the heat lowers cellular stress prior to and immediately following future exertional heat exposure","authors":"Ryan A. Dunn , Hui-Ying Luk , Casey R. Appell , Nigel C. Jiwan , Marcos S. Keefe , Jan-Joseph S. Rolloque , Yasuki Sekiguchi","doi":"10.1016/j.cstres.2024.05.001","DOIUrl":null,"url":null,"abstract":"<div>Muscle-damaging exercise (e.g., downhill running [DHR]) or heat exposure bouts potentially reduce physiological and/or cellular stress during future exertional heat exposure; however, the true extent of their combined preconditioning effects is unknown. Therefore, this study investigated the effect of muscle-damaging exercise in the heat on reducing physiological and cellular stress during future exertional heat exposure. Ten healthy males (mean ± Standard Definition; age, 23 ± 3 years; body mass, 78.7 ± 11.5 kg; height, 176.9 ± 4.7 cm) completed this study. Participants were randomly assigned into two preconditioning groups: (a) DHR in the heat (ambient temperature [Tamb], 35 °C; relative humidity [RH], 40%) and (b) DHR in thermoneutral (Tamb, 20 °C; RH, 20%). Seven days following DHR, participants performed a 45-min flat run in the heat (FlatHEAT [Tamb, 35 °C; RH, 40%]). During exercise, heart rate and rectal temperature (Trec) were recorded at baseline and every 5-min. Peripheral blood mononuclear cells were isolated to assess heat shock protein 72 (Hsp72) concentration between conditions at baseline, immediately post-DHR, and immediately pre-FlatHEAT and post-FlatHEAT. Mean Trec during FlatHEAT between hot (38.23 ± 0.38 °C) and thermoneutral DHR (38.26 ± 0.38 °C) was not significantly different (P = 0.68), with no mean heart rate differences during FlatHEAT between hot (172 ± 15 beats min−1) and thermoneutral conditions (174 ± 8 beats min−1; P = 0.58). Hsp72 concentration change from baseline to immediately pre-FlatHEAT was significantly lower in hot (−51.4%) compared to thermoneutral (+24.2%; P = 0.025) DHR, with Hsp72 change from baseline to immediately post-FlatHEAT also lower in hot (−52.6%) compared to thermoneutral conditions (+26.3%; P = 0.047). A bout of muscle-damaging exercise in the heat reduces cellular stress levels prior to and immediately following future exertional heat exposure.</div>","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":"29 3","pages":"Pages 472-482"},"PeriodicalIF":3.3000,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1355814524000737/pdfft?md5=7527cb8830b42a0ea4d640a6302a437a&pid=1-s2.0-S1355814524000737-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Stress & Chaperones","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1355814524000737","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Muscle-damaging exercise (e.g., downhill running [DHR]) or heat exposure bouts potentially reduce physiological and/or cellular stress during future exertional heat exposure; however, the true extent of their combined preconditioning effects is unknown. Therefore, this study investigated the effect of muscle-damaging exercise in the heat on reducing physiological and cellular stress during future exertional heat exposure. Ten healthy males (mean ± Standard Definition; age, 23 ± 3 years; body mass, 78.7 ± 11.5 kg; height, 176.9 ± 4.7 cm) completed this study. Participants were randomly assigned into two preconditioning groups: (a) DHR in the heat (ambient temperature [T_amb], 35 °C; relative humidity [RH], 40%) and (b) DHR in thermoneutral (T_amb, 20 °C; RH, 20%). Seven days following DHR, participants performed a 45-min flat run in the heat (Flat_HEAT [T_amb, 35 °C; RH, 40%]). During exercise, heart rate and rectal temperature (T_rec) were recorded at baseline and every 5-min. Peripheral blood mononuclear cells were isolated to assess heat shock protein 72 (Hsp72) concentration between conditions at baseline, immediately post-DHR, and immediately pre-Flat_HEAT and post-Flat_HEAT. Mean T_rec during Flat_HEAT between hot (38.23 ± 0.38 °C) and thermoneutral DHR (38.26 ± 0.38 °C) was not significantly different (P = 0.68), with no mean heart rate differences during Flat_HEAT between hot (172 ± 15 beats min⁻¹) and thermoneutral conditions (174 ± 8 beats min⁻¹; P = 0.58). Hsp72 concentration change from baseline to immediately pre-Flat_HEAT was significantly lower in hot (−51.4%) compared to thermoneutral (+24.2%; P = 0.025) DHR, with Hsp72 change from baseline to immediately post-Flat_HEAT also lower in hot (−52.6%) compared to thermoneutral conditions (+26.3%; P = 0.047). A bout of muscle-damaging exercise in the heat reduces cellular stress levels prior to and immediately following future exertional heat exposure.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

在高温下进行肌肉损伤性偏心运动，可在今后暴露于高温之前和之后立即降低细胞应力。

目的：肌肉损伤运动（如下坡跑[DHR]）或热暴露阵痛可能会减少未来用力-热暴露时的生理和/或细胞应激；然而，它们的综合预处理效应的真实程度尚不清楚。因此，本研究调查了在高温下进行肌肉损伤运动对降低未来体力消耗-热暴露期间的生理和细胞压力的影响：十名健康男性（平均值±SD；年龄，23±3 岁；体重，78.7±11.5 千克；身高，176.9±4.7 厘米）完成了本研究。参与者被随机分配到两个预处理组：a) 高温（环境温度[Tamb]，35°C；相对湿度[RH]，40%）下的 DHR；b) 中温（环境温度[Tamb]，20°C；相对湿度[RH]，20%）下的 DHR。DHR 后七天，参与者在高温下进行 45 分钟的平跑（FlatHEAT[Tamb, 35°C; RH, 40%]）。运动期间，在基线和每隔 5 分钟记录一次心率（HR）和直肠温度（Trec）。分离外周血单核细胞，以评估基线、DHR后立即、FlatHEAT前后立即等不同条件下的热休克蛋白72（Hsp72）浓度：高温（38.23±0.38oC）和中温 DHR（38.26±0.38oC）期间的平均 Trec 没有显著差异（p=0.68），高温（172±15beats.min-1）和中温（174±8beats.min-1；p=0.58）期间的平均 HR 没有差异。与热中性条件（+24.2%；p=0.025）相比，热条件下（-51.4%）的Hsp72浓度变化显著低于热条件下（+26.3%；p=0.047）的DHR，而与热中性条件（+26.3%；p=0.047）相比，热条件下（-52.6%）的Hsp72浓度变化也显著低于热条件下（-52.6%）的Hsp72浓度变化：结论：在高温下进行的肌肉损伤运动可在未来的体力热暴露之前和之后立即降低细胞应激水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Cell Stress & Chaperones 生物-细胞生物学

CiteScore

7.60

自引率

2.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.