Biogenic silver nanoparticle synthesis using orange peel extract and its multifaceted biomedical application.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI:10.1007/s00449-024-03031-2
Umme Hani, Fawziah Nasser Kidwan, Lamis Ahmed Albarqi, Saud Abduluziz Al-Qahtani, Ruba Muhammad AlHadi, Haifa Abdullah AlZaid, Nazima Haider, Mohammad Azam Ansari
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Abstract

The aim of this study was to employ an agro-industrial byproduct, specifically Citrus sinensis peels, as a reservoir of polyphenols. The natural chemicals present in C. sinensis peels serve as reducing agents in an environmentally benign method for synthesizing silver nanoparticles (AgNPs). This methodology not only provides a more environmentally friendly method for synthesizing nanoparticles but also enhances the value of agricultural waste, emphasizing the sustainable utilization of resources. In our study, AgNPs were successfully synthesized using peel aqueous exact of C. sinensis and then their various biological activity has been investigated. The synthesized AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy (TEM) analysis. Furthermore, their effectiveness in inhibiting growth and biofilm formation of Escherichia coli, Staphylococcus aureus, and Candida albicans has been investigated. The minimum inhibitory concentrations (MIC) for E. coli and S. aureus were both 32 μg/mL, and for C. albicans, it was 128 µg/mL. At 250 µg/mL of AgNPs, 94% and 92% biofilm inhibition were observed against E. coli and S. aureus, respectively. Furthermore, AgNPs demonstrated significant toxic effects against human prostate cancer cell line DU145 as investigated by anti-apoptotic, 4',6-diamidino-2-phenylindole (DAPI), reactive oxygen species (ROS), and acridine orange/ethidium bromide (AO/EtBr) assays. We also conducted uptake analysis on these pathogens and cancer cell lines to preliminarily investigate the mechanisms underlying their toxic effects. These findings confirm that AgNPs can serve as a cost-effective, non-toxic, and environmentally friendly resource for green synthesis of medicinal AgNPs. Moreover, this approach offers an alternative recycling strategy that contributes to the sustainable use of biological by-products.

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利用橙皮提取物合成生物银纳米粒子及其多方面的生物医学应用。
本研究的目的是利用一种农用工业副产品,特别是柑橘皮,作为多酚的宝库。柑橘皮中的天然化学物质可作为还原剂,以一种对环境无害的方法合成银纳米粒子(AgNPs)。这种方法不仅提供了一种更环保的合成纳米粒子的方法,还提高了农业废弃物的价值,强调了资源的可持续利用。在我们的研究中,我们成功地利用去皮水溶液合成了 AgNPs,并对其各种生物活性进行了研究。通过紫外-可见光谱、动态光散射(DLS)、扫描电子显微镜(SEM)、能量色散 X 射线(EDX)和透射电子显微镜(TEM)分析,对合成的 AgNPs 进行了表征。此外,还研究了它们抑制大肠杆菌、金黄色葡萄球菌和白色念珠菌生长和生物膜形成的效果。大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(MIC)均为 32 微克/毫升,而白色念珠菌的最低抑菌浓度为 128 微克/毫升。当 AgNPs 的浓度为 250 微克/毫升时,对大肠杆菌和金黄色葡萄球菌的生物膜抑制率分别为 94% 和 92%。此外,通过抗凋亡、4',6-二脒基-2-苯基吲哚(DAPI)、活性氧(ROS)和吖啶橙/溴化乙锭(AO/EtBr)检测,AgNPs 对人类前列腺癌细胞株 DU145 具有显著的毒性作用。我们还对这些病原体和癌细胞系进行了吸收分析,以初步研究其毒性作用的机制。这些研究结果证实,AgNPs 可以作为一种经济、无毒、环保的资源,用于药用 AgNPs 的绿色合成。此外,这种方法还提供了另一种回收策略,有助于生物副产品的可持续利用。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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