Addition of nisin to high-viscosity glass-ionomer cement: a comparative in vitro study on antibacterial and physical properties.

IF 2.3 Q2 DENTISTRY, ORAL SURGERY & MEDICINE European Archives of Paediatric Dentistry Pub Date : 2024-08-01 Epub Date: 2024-05-14 DOI:10.1007/s40368-024-00910-w
D Hegde, B S Suprabha, K Ginjupalli, E Suman, S Natarajan, R Shenoy, A Rao
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Abstract

Purpose: Nisin is a lantibiotic effective against Gram-positive microorganisms such as Streptococcus mutans. The study aimed to determine the effect of the addition of nisin to high-viscosity glass-ionomer cement (HVGIC) on its antibacterial activity, setting time, surface microhardness, and compressive strength.

Methods: 1 and 3% w/w nisin were added to HVGIC before mixing. Unmodified HVGIC was the control. Agar disc diffusion, direct contact test, and scanning electron microscopy (SEM) analysis were used to evaluate antibacterial activity against S. mutans. Setting time, surface microhardness, and compressive strength were measured using Gilmore needle apparatus, digital microhardness tester, and universal testing machine, respectively. Statistical analysis included Student's t test, one-way ANOVA with Tamhane's post hoc test, and repeated-measures ANOVA.

Results: As evidenced by the agar disc diffusion (p < 0.001), direct contact tests (p = 0.025), and SEM analysis of the S. mutans cell count and cell surface area (p = 0.049 and 0.003), 3% nisin had the strongest antibacterial activity. There was a dose-dependent increase in setting time (p = 0.005) and surface microhardness (p = 0.006), with no significant difference in compressive strength compared to control.

Conclusion: The addition of 3% nisin to HVGIC enhances the antibacterial action against S. mutans and surface microhardness without adversely affecting setting time and compressive strength.

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在高粘度玻璃-离子水泥中添加尼生素:抗菌和物理特性的体外对比研究。
目的:Nisin 是一种抗菌素,对变异链球菌等革兰氏阳性微生物有效。本研究旨在确定在高粘度玻璃-离子水泥(HVGIC)中添加尼生素对其抗菌活性、凝结时间、表面微硬度和抗压强度的影响。未改性的 HVGIC 为对照组。采用琼脂盘扩散、直接接触试验和扫描电子显微镜(SEM)分析来评估对突变菌的抗菌活性。凝固时间、表面显微硬度和抗压强度分别使用吉尔莫尔针刺仪、数字显微硬度计和万能试验机进行测量。统计分析包括学生 t 检验、单因素方差分析和重复测量方差分析:结果:琼脂盘扩散试验(p < 0.001)、直接接触试验(p = 0.025)、SEM 分析(p = 0.049 和 0.003)显示,3% 尼生素的抗菌活性最强。与对照组相比,凝固时间(p = 0.005)和表面微硬度(p = 0.006)呈剂量依赖性增加,抗压强度无显著差异:结论:在 HVGIC 中添加 3% 的 nisin 可增强对 S. mutans 的抗菌作用和表面微硬度,而不会对凝固时间和抗压强度产生不利影响。
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来源期刊
European Archives of Paediatric Dentistry
European Archives of Paediatric Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
4.40
自引率
9.10%
发文量
81
期刊介绍: The aim and scope of European Archives of Paediatric Dentistry (EAPD) is to promote research in all aspects of dentistry for children, including interceptive orthodontics and studies on children and young adults with special needs. The EAPD focuses on the publication and critical evaluation of clinical and basic science research related to children. The EAPD will consider clinical case series reports, followed by the relevant literature review, only where there are new and important findings of interest to Paediatric Dentistry and where details of techniques or treatment carried out and the success of such approaches are given.
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