Beam shaping in light-sheet microscopy: an experimental analysis

IF 4.6 Q1 OPTICS Journal of Physics-Photonics Pub Date : 2024-05-12 DOI:10.1088/2515-7647/ad46a9
Manuel Hüpfel and Gerd Ulrich Nienhaus
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Abstract

Thanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution. For light sheet generation with scanned laser beams, holographic beam shaping offers precise control over the optical fields exciting the fluorescence. Various illumination schemes have been proposed, aiming for best image quality with regard to axial resolution, optical sectioning, illumination homogeneity and photobleaching while at the same time retaining a large field of view. Here, we have engineered and characterized a variety of beams and analyzed their imaging performance by using phantom samples and zebrafish embryos. These data may assist researchers to select the light sheet best suited to the imaging application at hand.
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光片显微镜中的光束整形:实验分析
由于具有独特的光学切片功能,光片荧光显微镜已被证明是一种强大的技术,可对整个模型生物体进行高空间和时间分辨率的体积成像。使用扫描激光束产生光片时,全息光束整形可精确控制激发荧光的光场。目前已经提出了各种照明方案,目的是在保留大视野的同时,在轴向分辨率、光学切片、照明均匀性和光漂白方面获得最佳图像质量。在此,我们设计了多种光束并对其进行了表征,还利用模型样本和斑马鱼胚胎分析了它们的成像性能。这些数据可帮助研究人员选择最适合手头成像应用的光片。
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来源期刊
CiteScore
10.70
自引率
0.00%
发文量
27
审稿时长
12 weeks
期刊最新文献
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