Beam shaping in light-sheet microscopy: an experimental analysis

IF 4.6 Q1 OPTICS Journal of Physics-Photonics Pub Date : 2024-05-12 DOI:10.1088/2515-7647/ad46a9
Manuel Hüpfel and Gerd Ulrich Nienhaus
{"title":"Beam shaping in light-sheet microscopy: an experimental analysis","authors":"Manuel Hüpfel and Gerd Ulrich Nienhaus","doi":"10.1088/2515-7647/ad46a9","DOIUrl":null,"url":null,"abstract":"Thanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution. For light sheet generation with scanned laser beams, holographic beam shaping offers precise control over the optical fields exciting the fluorescence. Various illumination schemes have been proposed, aiming for best image quality with regard to axial resolution, optical sectioning, illumination homogeneity and photobleaching while at the same time retaining a large field of view. Here, we have engineered and characterized a variety of beams and analyzed their imaging performance by using phantom samples and zebrafish embryos. These data may assist researchers to select the light sheet best suited to the imaging application at hand.","PeriodicalId":44008,"journal":{"name":"Journal of Physics-Photonics","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Physics-Photonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1088/2515-7647/ad46a9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0

Abstract

Thanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution. For light sheet generation with scanned laser beams, holographic beam shaping offers precise control over the optical fields exciting the fluorescence. Various illumination schemes have been proposed, aiming for best image quality with regard to axial resolution, optical sectioning, illumination homogeneity and photobleaching while at the same time retaining a large field of view. Here, we have engineered and characterized a variety of beams and analyzed their imaging performance by using phantom samples and zebrafish embryos. These data may assist researchers to select the light sheet best suited to the imaging application at hand.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
光片显微镜中的光束整形:实验分析
由于具有独特的光学切片功能,光片荧光显微镜已被证明是一种强大的技术,可对整个模型生物体进行高空间和时间分辨率的体积成像。使用扫描激光束产生光片时,全息光束整形可精确控制激发荧光的光场。目前已经提出了各种照明方案,目的是在保留大视野的同时,在轴向分辨率、光学切片、照明均匀性和光漂白方面获得最佳图像质量。在此,我们设计了多种光束并对其进行了表征,还利用模型样本和斑马鱼胚胎分析了它们的成像性能。这些数据可帮助研究人员选择最适合手头成像应用的光片。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
10.70
自引率
0.00%
发文量
27
审稿时长
12 weeks
期刊最新文献
Wavefront shaping simulations with augmented partial factorization An efficient compact blazed grating antenna for optical phased arrays Highly reflective and high-Q thin resonant subwavelength gratings A practical guide to digital micro-mirror devices (DMDs) for wavefront shaping A modular GUI-based program for genetic algorithm-based feedback-assisted wavefront shaping
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1