Reactive extraction for the separation of glyceric acid from aqueous solutions with 2-naphthaleneboronic acid and tri-octyl methyl ammonium chloride

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-05-10 DOI:10.1007/s12257-024-00110-9
Long Hoang Dang Bui, Keitaro Aoki, Tomonari Tanaka, Yuji Aso
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Abstract

Glyceric acid (GA), a carboxylic group-containing diol, is obtained from bioresources via microbial processes. In this study, we aimed to develop a reactive extraction method to separate GA from aqueous solutions using 2-naphthaleneboronic acid (2NB) and tri-octyl methyl ammonium chloride (TOMAC). Different feed molar amounts of 2NB (0–25 µmol), TOMAC (0–500 µmol), and NaOH (0–250 µmol) were used for GA (2.5 µmol) separation. A combination of 25 µmol 2NB, 100 µmol TOMAC, and 25 µmol NaOH was determined to be optimal for GA separation, providing 66.8 ± 3.2% GA yield at pH 11. GA was extracted by 2NB and TOMAC in a coordinated manner. Moreover, effects of various carboxylic acids (acetic, lactic, succinic, malic, tartaric, and citric acids) on GA separation from aqueous solutions were investigated. Interestingly, no significant effect on GA yeild (60.3 ± 1.2–65.2 ± 2.5%) was observed regardless of the type of carboxylic acid. The optimized protocol was subsequently applied to separate GA from crude GA solution prepared by incubating glycerol with the cells of the acetic acid bacterium, Acetobacter tropicalis NBRC 16470. GA separation was achieved at a comparable level (yield: 70.6 ± 4.6% and purity: 76.1 ± 4.1%) as that achieved using a GA reagent. This study demonstrated the efficiency of the repeated use of the organic phase for GA separation, with no significant changes in GA yield. Query Text="Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Author 1 Given name: [specify authors given name] Last name [specify authors last name]. Also, kindly confirm the details in the metadata are correct."

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用 2-萘硼酸和三辛基甲基氯化铵反应萃取分离水溶液中的甘油酸
甘油酸(GA)是一种含羧基的二元醇,可通过微生物过程从生物资源中获得。在这项研究中,我们旨在开发一种反应萃取法,利用 2-萘硼酸(2NB)和三辛基甲基氯化铵(TOMAC)从水溶液中分离出甘油酸。在分离 GA(2.5 µmol)时,使用了不同进料摩尔量的 2NB(0-25 µmol)、TOMAC(0-500 µmol)和 NaOH(0-250 µmol)。25微摩尔2NB、100微摩尔TOMAC和25微摩尔NaOH的组合被确定为分离GA的最佳组合,在pH值为11时,GA的产率为66.8 ± 3.2%。2NB 和 TOMAC 以协调的方式提取了 GA。此外,还研究了各种羧酸(乙酸、乳酸、琥珀酸、苹果酸、酒石酸和柠檬酸)对从水溶液中分离 GA 的影响。有趣的是,无论使用哪种羧酸,对 GA 的酵母率(60.3 ± 1.2-65.2 ± 2.5%)都没有明显影响。优化后的方案随后被用于从通过甘油与醋酸细菌(Acetobacter tropicalis NBRC 16470)细胞培养制备的粗 GA 溶液中分离 GA。GA 的分离率(产率:70.6 ± 4.6%,纯度:76.1 ± 4.1%)与使用 GA 试剂的分离率相当。这项研究证明了重复使用有机相分离 GA 的效率,GA 产率没有显著变化。Query Text="请确认作者姓名是否准确,顺序是否正确(名字、中间名/首字母、姓氏)。作者 1 姓:[请注明作者姓名]。另外,请确认元数据中的细节是否正确"。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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