Functionalized magnetic nanomaterials as recyclable adsorbents for efficient flavonoid enrichment in Scutellaria Radix

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography B Pub Date : 2024-05-10 DOI:10.1016/j.jchromb.2024.124156

Guohua Teng , Hezhao Bai , Chen Zhang , Jingyi Yang , Xiaoye Wang , Yu Zhu , Fei Tian

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Abstract

A magnetic composite (Fe₃O₄@SiO₂@PNIPAM-co-NHMA) with high adsorption capacity and recoverability was developed for the enrichment and determination of flavonoids in Scutellaria Radix (SR). A magnetic solid-phase extraction (MSPE) technique using Fe₃O₄@SiO₂@PNIPAM-co-NHMA absorbent in combination with high-performance liquid chromatography (HPLC) was developed for selectively enrichment and determination of the biologically active flavonoids in the aqueous extract of SR, including baicalein, baicalin, wogonoside and wogonin. Under the optimized experimental conditions, the magnetic adsorbent could adsorb up to 77.0 ± 0.98 % − 98.15 ± 0.15 % of four representative flavonoids from SR, with elution rates varying from 55.10 ± 0.25 % to 91.94 ± 1.85 %. The limits of detection (LOD) and limits of quantitation (LOQ) were 0.01–0.35 μg/mL and 0.03–0.98 μg/mL, respectively. In addition, it remained effective after six replicates, demonstrating its potential as a recoverable adsorbent for enriching flavonoids in traditional Chinese medicine.

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功能化磁性纳米材料作为可回收吸附剂用于高效富集黄芩中的黄酮类化合物

为富集和测定黄芩中的黄酮类化合物，研究人员开发了一种具有高吸附能力和高回收率的磁性复合材料（Fe3O4@SiO2@PNIPAM-co-NHMA）。利用Fe3O4@SiO2@PNIPAM-co-NHMA吸附剂结合高效液相色谱法（HPLC）开发了一种磁性固相萃取（MSPE）技术，用于选择性富集和测定黄芩水提取物中具有生物活性的黄酮类化合物，包括黄芩素、黄芩苷、沃柑苷和沃柑素。在优化的实验条件下，磁性吸附剂对SR中4种代表性黄酮类化合物的吸附率为77.0 ± 0.98 % - 98.15 ± 0.15 %，洗脱率为55.10 ± 0.25 % - 91.94 ± 1.85 %。检测限（LOD）和定量限（LOQ）分别为 0.01-0.35 μg/mL 和 0.03-0.98 μg/mL。此外，经过六次重复后，它仍然有效，这表明它具有作为可回收吸附剂富集中药中黄酮类化合物的潜力。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.