Exosome-mimetic vesicles derived from fibroblasts carrying matrine for wound healing.

IF 6.3 1区医学 Q1 DERMATOLOGY Burns & Trauma Pub Date : 2024-05-15 eCollection Date: 2024-01-01 DOI:10.1093/burnst/tkae015

Xinyue Zhang, Jiahua Huang, Jing Zhao, Lisha Li, Fengze Miao, Tingrui Zhang, Zhongjian Chen, Xing Zhou, Zongguang Tai, Quangang Zhu

{"title":"Exosome-mimetic vesicles derived from fibroblasts carrying matrine for wound healing.","authors":"Xinyue Zhang, Jiahua Huang, Jing Zhao, Lisha Li, Fengze Miao, Tingrui Zhang, Zhongjian Chen, Xing Zhou, Zongguang Tai, Quangang Zhu","doi":"10.1093/burnst/tkae015","DOIUrl":null,"url":null,"abstract":"Background: Chronic skin wounds are a leading cause of hospital admissions and reduced life expectancy among older people and individuals with diabetes. Delayed wound healing is often attributed to a series of cellular abnormalities. Matrine, a well-studied component found in Sophora flavescens, is recognized for its anti-inflammatory effects. However, its impact on wound healing still remains uncertain. This study aims to explore the potential of matrine in promoting wound healing.Methods: In this study, we utilized gradient extrusion to produce fibroblast-derived exosome-mimetic vesicles as carriers for matrine (MHEM). MHEM were characterized using transmission electron microscopy and dynamic light scattering analysis. The therapeutic effect of MHEM in wound healing was explored in vitro and in vivo.Results: Both matrine and MHEM enhanced the cellular activity as well as the migration of fibroblasts and keratinocytes. The potent anti-inflammatory effect of matrine diluted the inflammatory response in the vicinity of wounds. Furthermore, MHEM worked together to promote angiogenesis and the expression of transforming growth factor β and collagen I. MHEM contained growth factors of fibroblasts that regulated the functions of fibroblasts, keratinocytes and monocytes, which synergistically promoted wound healing with the anti-inflammatory effect of matrine.Conclusions: MHEM showed enhanced therapeutic efficacy in the inflammatory microenvironment, for new tissue formation and angiogenesis of wound healing.","PeriodicalId":9553,"journal":{"name":"Burns & Trauma","volume":"12 ","pages":"tkae015"},"PeriodicalIF":6.3000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11095412/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Burns & Trauma","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/burnst/tkae015","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Chronic skin wounds are a leading cause of hospital admissions and reduced life expectancy among older people and individuals with diabetes. Delayed wound healing is often attributed to a series of cellular abnormalities. Matrine, a well-studied component found in Sophora flavescens, is recognized for its anti-inflammatory effects. However, its impact on wound healing still remains uncertain. This study aims to explore the potential of matrine in promoting wound healing.

Methods: In this study, we utilized gradient extrusion to produce fibroblast-derived exosome-mimetic vesicles as carriers for matrine (MHEM). MHEM were characterized using transmission electron microscopy and dynamic light scattering analysis. The therapeutic effect of MHEM in wound healing was explored in vitro and in vivo.

Results: Both matrine and MHEM enhanced the cellular activity as well as the migration of fibroblasts and keratinocytes. The potent anti-inflammatory effect of matrine diluted the inflammatory response in the vicinity of wounds. Furthermore, MHEM worked together to promote angiogenesis and the expression of transforming growth factor β and collagen I. MHEM contained growth factors of fibroblasts that regulated the functions of fibroblasts, keratinocytes and monocytes, which synergistically promoted wound healing with the anti-inflammatory effect of matrine.

Conclusions: MHEM showed enhanced therapeutic efficacy in the inflammatory microenvironment, for new tissue formation and angiogenesis of wound healing.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

成纤维细胞提取的外泌体模拟囊泡携带促进伤口愈合的马钱子碱。

背景：慢性皮肤伤口是老年人和糖尿病患者入院治疗和预期寿命缩短的主要原因。伤口延迟愈合通常归因于一系列细胞异常。马钱子碱是一种在洋槐中发现的成分，其抗炎作用已得到广泛研究。然而，它对伤口愈合的影响仍不确定。本研究旨在探索洋槐黄素在促进伤口愈合方面的潜力：在这项研究中，我们利用梯度挤压法生产了成纤维细胞衍生的外泌体模拟囊泡作为马钱子碱的载体（MHEM）。利用透射电子显微镜和动态光散射分析对 MHEM 进行了表征。在体外和体内探讨了 MHEM 对伤口愈合的治疗效果：结果：马钱子碱和 MHEM 都能增强成纤维细胞和角质细胞的细胞活性和迁移能力。马钱子碱的强效抗炎作用能减轻伤口附近的炎症反应。此外，MHEM 还能共同促进血管生成，促进转化生长因子 β 和胶原蛋白 I 的表达。MHEM 含有成纤维细胞生长因子，能调节成纤维细胞、角质形成细胞和单核细胞的功能，与马特林的抗炎作用协同促进伤口愈合：MHEM在炎症微环境中显示出更强的疗效，可促进伤口愈合中新组织的形成和血管生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Burns & Trauma 医学-皮肤病学

CiteScore

8.40

自引率

9.40%

发文量

186

审稿时长

6 weeks

期刊介绍： The first open access journal in the field of burns and trauma injury in the Asia-Pacific region, Burns & Trauma publishes the latest developments in basic, clinical and translational research in the field. With a special focus on prevention, clinical treatment and basic research, the journal welcomes submissions in various aspects of biomaterials, tissue engineering, stem cells, critical care, immunobiology, skin transplantation, and the prevention and regeneration of burns and trauma injuries. With an expert Editorial Board and a team of dedicated scientific editors, the journal enjoys a large readership and is supported by Southwest Hospital, which covers authors'' article processing charges.