Novel synthesis of trans-ferulic acid loaded tragacanth-chitosan nanoformulations and evaluation of their biological activities

Abstract

Trans-ferulic acid, is one of the many biologically significant bioactive components of Datura. It has wide range of potential applications in the food industry, health, and cosmetics industries. Trans-ferulic acid possess myriad properties like anti-inflammatory, anti-aging, antiangiogenic, anticancer, antimicrobial, and antioxidant properties. To exploit its antioxidant and anti-inflammatory properties, first of all, trans-ferulic acid loaded tragacanth/chitosan nanoparticles (TFA loaded TGCA-NPs) were synthesized by ionic gelation method using tragacanth and chitosan as polymers. Tragacanth used to encapsulate the trans-ferulic acid is a polysaccharide gum, which is used all over the world in industries ranging from the food industry to healthcare systems due to its availability, affordability, non-toxicity, and environmental friendliness. The synthesized nanoparticles were then characterized by using techniques like FTIR, UV–Vis Spectroscopy, SEM and TEM. The particle size of nanoparticles thus obtained ranged from 105 nm to 250 nm. SEM micrograph of nanoparticles showed tiny rod-like structures, which appear to be coated on the polymer. TEM images showed somewhat sphere-shaped nanoparticles with a projecting rod-like structure that might be due to the trans-ferulic acid encapsulation in tragacanth-chitosan polymer. Decent antimicrobial activity of synthesized nanoparticles was observed against pathogenic bacteria named Staphylococcus aureus. Hemocompatibility of trans-ferulic acid loaded tragacanth-chitosan nanoparticles was also examined. The antioxidant activity was evaluated by DPPH radical scavenging assay, in which synthesized nanoparticles showed 41.62 % radical scavenging activity. For in-vitro anti-inflammatory activity Human Red Blood Cell membrane stabilization method (HRBC method) was used, and the results showed higher protection by the synthesized nanoparticles in comparison to the pure trans-ferulic acid and blank nanoparticles.