Analysis of genetic variability among regional mango varieties grown in Rajshahi district using RAPD markers

Abstract

The evaluation of genetic relatedness and diversity is a crucial step in describing and utilizing germplasm for the development of new varieties. In this study, the genetic diversity of four indigenous mango varieties was investigated. Three RAPD primers were used to perform the DNA fingerprint after the DNA was extracted from the leaf sample using the modified CTAB procedure. Cluster analysis was carried out using the unweighted pair-group method of arithmetic means (UPGMA). According to the results, the highest amount of DNA was recovered from the Fazli cultivar (1142.7 ± 0.6 ng/μl). On amplification, the RAPD analysis revealed 25 scoreable bands, of which twenty (20) were monomorphic and five (5) were polymorphic. The primer OPA-03 had the highest polymorphism of 23 %, whereas OPD-04 had no band. The Amropali versus Fazli variety pair had the highest linkage distance (4.69), whilst the Ashawina versus Lengra variety pair had the lowest linkage distance (2.00). At a linkage distance of 4.25, the dendrogram revealed the segregation of the four mango cultivars into two major clusters. Amropali was placed in cluster 1 (C1) and Ashawina, Fazli, and Lengra were placed in cluster 2 (C2) demonstrates that the Amropali variety is distinct from all others. According to the findings, this research could potentially provide insight about the regional mango diversity and be helpful for future mango breeding programs.