Investigation of Peste des petits ruminants virus circulation in Uttarakhand, India: a step towards global eradication of PPR by 2030.

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES Veterinaria italiana Pub Date : 2024-03-31 DOI:10.12834/VetIt.2988.21721.3

Amisha Netam, Anuj Tewari, Rajesh Kumar

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Abstract

This study was conducted to estimate the seroprevalence of Peste des petits ruminants virus (PPRV) and to determine the virus distribution in unvaccinated goats in the Pantnagar region of Uttarakhand state, India. A total of 212 serum samples from goats were collected randomly from various villages in three districts (Udhamsingh Nagar, Nainital, and Almora) of Uttarakhand. Serum samples were tested for anti-PPRV antibodies by a commercially available kit. RNA was extracted from the clinical samples and it was subjected to one-step RT-PCR, followed by virus isolation from positive samples. A total of 41 animals from various villages were found to be seropositive with a prevalence rate of 19.33%. PPR outbreaks were also reported from the Tarai region of Uttarakhand, and detection by PCR confirmed PPRV in 8 goats. Two representative swab samples were subjected to virus isolation in Vero cells and both samples showed typical cytopathic effects. The present study shows that PPRV is circulating in the Tarai region of Uttarakhand and mass vaccination for PPR must be followed in this region to increase herd immunity to a protective level. To the best of our knowledge, this is the first investigation of PPRV seroprevalence in unvaccinated goats of Uttarakhand, India.

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印度北阿坎德邦小反刍兽疫病毒传播调查：向 2030 年全球根除小反刍兽疫迈出的一步。

本研究旨在估计印度北阿坎德邦潘特纳加地区小反刍兽疫病毒（PPRV）的血清流行率，并确定病毒在未接种疫苗的山羊中的分布情况。研究人员从北阿坎德邦三个地区（乌达姆辛纳加尔、奈尼塔尔和阿尔莫拉）的多个村庄随机采集了 212 份山羊血清样本。使用市售试剂盒对血清样本进行抗 PPRV 抗体检测。从临床样本中提取 RNA，进行一步式 RT-PCR 检测，然后从阳性样本中分离病毒。结果发现，各村共有 41 头动物的血清反应呈阳性，发病率为 19.33%。Uttarakhand 的 Tarai 地区也报告了 PPR 疫情，通过 PCR 检测证实 8 只山羊感染了 PPRV。两份具有代表性的拭子样本在 Vero 细胞中进行了病毒分离，两份样本均显示出典型的细胞病理效应。本研究表明，PPRV 在北阿坎德邦的塔雷地区流行，因此必须在该地区大规模接种 PPR 疫苗，以将羊群免疫力提高到保护水平。据我们所知，这是首次对印度北阿坎德邦未接种疫苗山羊的 PPRV 血清流行率进行调查。

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来源期刊

Veterinaria italiana VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.