Comparison of culture media for the detection of Legionella spp. in sanitary water samples: Adaptation to ISO 11731:2017

Enfermedades infecciosas y microbiologia clinica (English ed.) Pub Date : 2024-08-01 DOI:10.1016/j.eimce.2024.05.007

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Abstract

Introduction

Water sample culturing is the reference method to detect Legionella spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC medium, which inhibits interfering microbiota but hinders the growth of Legionella spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.

Methods

We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of Legionella spp. detected in both media.

Results

The median in BCYE was 2000 cfu/L higher than in GVPC (P = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence or low interfering microbiota BCYE was four times more sensitive than GVPC (P = .000).

Conclusion

Including BCYE in the analysis of sanitary water significantly improves the recovery of Legionella spp. in low contaminated samples.

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用于检测卫生水样中军团菌属的培养基比较：适应 ISO 11731:2017。

简介：水样培养是检测卫生设施中军团菌属的参考方法。在 2017 年之前，UNE-EN ISO 11731 仅包括 GVPC 培养基，该培养基可抑制干扰微生物群，但阻碍军团菌的生长。为了提高其回收率，新标准将 BCYE 培养基纳入了工作方案：我们按照认可的内部程序将 1306 份卫生水样接种到 BCYE 和 GVPC 上。我们比较了两种培养基中检测到的军团菌的 cfu/L 数量：结果：BCYE 中的中位数比 GVPC 中高 2000 cfu/L（P = .000）。在存在大量干扰微生物群的情况下，两种培养基的灵敏度相似；在没有干扰微生物群或干扰微生物群较少的情况下，BCYE 的灵敏度是 GVPC 的四倍（P = .000）：结论：在卫生用水分析中加入 BCYE 可显著提高低污染样本中军团菌属的回收率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Enfermedades infecciosas y microbiologia clinica (English ed.)

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