Novel R2R3-MYB Transcription Factor LhMYB1 Promotes Anthocyanin Accumulation in Lilium concolor var. pulchellum

IF 3.1 3区农林科学 Q1 HORTICULTURE Horticulturae Pub Date : 2024-05-15 DOI:10.3390/horticulturae10050509

Shengnan Tian, Muhammad Moaaz Ali, Mingli Ke, Yuxian Lu, Yiping Zheng, Xuanmei Cai, Shaozhong Fang, Jian Wu, Zhimin Lin, Faxing Chen

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Abstract

Lilium concolor var. pulchellum has a brilliant flower colour, high germination rate, and resistance to cold, drought, and salinity and is an excellent source of lily germplasm. Anthocyanins are important flavonoids commonly found in plants and can make the flowers and fruits of plants more colourful. We first found that 0.2 mg/L 1-naphthaleneacetic acid (NAA) specifically induced the accumulation of anthocyanins, which were mainly cyanidins, in callus tissue culture of Lilium. Transcriptomic results indicated that anthocyanin accumulation was mainly involved in the flavonoid pathway, and an LhMYB1 transcription factor encoding 267 amino acids positively associated with anthocyanin accumulation was cloned from the MYB family. Subcellular localisation in tobacco showed that the gene was located in the nucleus of epidermal cells. Virus-induced gene silencing showed that silencing of the LhMYB1 gene on lily petals resulted in a purple to white colour change and a decrease in anthocyanin deposition, mainly in the upper and lower epidermis of the petals. Therefore, the results of this study will provide some ideas for the molecular breeding of lily flower colour.

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新型 R2R3-MYB 转录因子 LhMYB1 促进百合变种的花青素积累

Lilium concolor var. pulchellum 花色艳丽，发芽率高，耐寒、耐旱、耐盐碱，是百合种质的优良来源。花青素是植物中常见的重要黄酮类化合物，可使植物的花朵和果实更加艳丽。我们首先发现，0.2 mg/L 1-萘乙酸（NAA）能特异性诱导百合胼胝体组织培养中花青素的积累，其中主要是花青素。转录组学结果表明，花青素的积累主要涉及类黄酮途径，并从 MYB 家族中克隆了一个编码 267 个氨基酸的 LhMYB1 转录因子，该转录因子与花青素的积累呈正相关。烟草亚细胞定位表明，该基因位于表皮细胞的细胞核中。病毒诱导的基因沉默显示，沉默百合花瓣上的 LhMYB1 基因会导致花瓣颜色由紫色变为白色，花青素沉积减少，主要集中在花瓣的上表皮和下表皮。因此，本研究的结果将为百合花色的分子育种提供一些思路。

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