Incidence of Cryptosporidium: A Neglected Parasite in Immunocompetent Adults in Swabi

Amjad Mahboob, Nazish Babar, Asim Ali Shah, Asfandyar Babar, Amir Mumtaz, Aqsa Aslam
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Abstract

Objective: To highlight the increasing incidence of Cryptosporidium in immunocompetent adults and the resultant altered epidemiology. Methodology: This study was conducted over a period of one year, from 1st January 2023 to 1st of January, 2024 at Microbiology Laboratory MTI- GKMC/BKMC, Swabi. Modified Ziehl Neelsen staining was performed on the received stool samples from patients presenting with wide range of symptoms associated with Irritable Bowel Syndrome (IBS). Abdominal pain, recurrent episodes of diarrhea and weight loss were the main symptoms. Modified ZN stain being one of the most reliable, quick and cost effective methods for the detection of cysts of Cryptosporidium using 40X and oil immersion lens was used for diagnosis. Results: A total of 62 samples were received from immunocompetent patients in the said period out of which 34% were positive for the oocysts of Cryptosporidium species. Out of a total of 21 positive samples 62% were males and 38% were females. The age range of the patients spanned between 20 to 60 years. Conclusion: Knowing this high incidence 34% of Cryptosporidium spp. in the healthy adults, necessitates to prioritize steps for the prevention of this infection by implementation of public health measures.
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隐孢子虫的发病率:斯瓦比免疫功能健全的成年人中被忽视的寄生虫
目的强调隐孢子虫在免疫功能健全的成年人中发病率的增加以及由此导致的流行病学的改变:本研究于 2023 年 1 月 1 日至 2024 年 1 月 1 日在斯瓦比的 MTI- GKMC/BKMC 微生物实验室进行,为期一年。研究人员对收到的粪便样本进行了改良齐氏尼尔森染色法检测,这些样本来自出现多种肠易激综合征(IBS)相关症状的患者。腹痛、反复腹泻和体重减轻是主要症状。改良 ZN 染色法是检测隐孢子虫囊蚴的最可靠、快速和经济有效的方法之一,使用 40X 和油浸透镜进行诊断:结果:在上述期间共收到 62 份来自免疫功能正常患者的样本,其中 34% 的样本对隐孢子虫卵囊呈阳性反应。在总共 21 份阳性样本中,62% 为男性,38% 为女性。患者年龄介于 20 岁至 60 岁之间:健康成年人中隐孢子虫的发病率高达 34%,因此有必要采取公共卫生措施,优先预防隐孢子虫感染。
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