Phytochemical screening and antimicrobial activity of some medicinal plants

Alaa Hamady Obeid Al-Taei, R. Saleh, S. Kadhum, A. Omran, R. M. Ewadh, H. H. Owadh
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Abstract

The antimicrobial activity of the aqueous and ethanolic extracts of Myrtus communis, Ammi visnaga, and Equisetum arvense was investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar well diffusion method. Serial concentrations (15%, 30%, and 50%) of the extracts of each plant were tested and compared with gentamicin (10 μg) and fluconazole (25 μg). Most of the extract concentrations showed a relatively high antimicrobial activity against all the tested microbes, and the ethanolic extract was more effective than the aque¬ous extract. The activity of plant extracts increased with the increasing extract concentration of Myrtus communis, which appeared to possess a more antimicrobial activity than the other plants assessed; in fact, its ethanolic extract exhibited the highest inhibition zone against S. aureus (32 mm). The ethanolic plant extracts at a concentration of 50% displayed the maximum activity against the herein assessed isolates. Moreover, E. coli showed a higher sensi¬tivity to most extracts, while the lowest effect being noticed on C. albicans.
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一些药用植物的植物化学筛选和抗菌活性
采用琼脂井扩散法研究了桃金娘、Ammi visnaga 和马钱子的水提取物和乙醇提取物对金黄色葡萄球菌、大肠杆菌和白色念珠菌的抗菌活性。测试了每种植物提取物的序列浓度(15%、30% 和 50%),并与庆大霉素(10 微克)和氟康唑(25 微克)进行了比较。大多数浓度的提取物对所有测试微生物都显示出较高的抗菌活性,乙醇提取物比水提取物更有效。随着香桃木提取物浓度的增加,植物提取物的活性也随之增加,香桃木的抗菌活性似乎高于其他被评估的植物;事实上,其乙醇提取物对金黄色葡萄球菌的抑制区最大(32 毫米)。浓度为 50%的植物乙醇提取物对所评估的分离物具有最大的活性。此外,大肠杆菌对大多数提取物的敏感性较高,而对白喉杆菌的作用最低。
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