Antiadipogenic activity of homoeopathic preparation of Chelidonium Majus employing3T3-L1 cell line as a model.

Abstract

Adipocytes are derived from mesenchymal stem cells through the process of adipogenesis. Adipocyte metabolism is abnormal in a range of disorders like obesity, nutritional insufficiency and diabetes. The 3T3-L1 preadipocytes are most often used to create adipocyte models, and they can be differentiated into adipocyte cells under the right conditions. Homeopathic preparation of Chelidonium majus (HPCM) in management of Obesity and T2DM has many evidences in clinical practice.3T3-L1 preadipocyte cells were differentiated into adipocytes by using differentiation cocktail. Cell were treated with HPCM attenuation at the concentration of 0.5%, 1% and 2% for 15 days. Oil O Red Staining was used to assess LD accumulation. In order to determine the lipid content in 3T3-L1 adipocytes, cells were dissolved in isopropanol and the absorbance values were measured. Images were captured and analysed using software imageJ.We investigated the action of Homeopathic preparations of Chelidonium majus (HPCM) in obesity using the 3T3-L1 adipogenesis model employing huMSC’s.After differentiation of adipocytes many LDs were formed in 3T3-L1 preadipocytes which can be compared with no lipid droplet in 3T3-L1nondifferentiated cells. Further, area of differentiated adipocytes was mapped and then compared for LD accumulation in control and HPCM for checking its antiadipogenic activity.A significant reduction in accumulation of lipid droplets was seen in 0.5%, 1% and 2% concentration of HPCM as compared to control during the differentiation of 3T3-L1 preadipocytes into adipocytes. Excessive differentiation of cells and high fat accumulation in the adipose tissue are closely linked to obesity. Preadipocyte differentiation inhibitors may have preventive and therapeutic potential as anti-obesity drugs. HPCM has potential to act as an antiadipogenic agent which can be used to combat various diseases like obesity, T2DM and cardiovascular diseases