A. A. Aldandan, Mohamed H El-Kenawy, Abdullah A. Al-Sharif, Eman T. Hamam, A. Badr
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引用次数: 0
Abstract
The role of Acetyl -11-keto-β-boswellic acid (AKBA) in regulating osteoblast differentiation was recently brought to light. Therefore, the current study was designed to explore the osteogenic differentiation capability of AKBA on bone marrow mesenchymal stem cells (BMMSCs) as a potential therapeutic agent to accelerate the healing process in apicoectomy.
BMMSCs were characterized by flow cytometry. Cellular viability and proliferation assays were used with different concentrations of AKBA. Cells were divided into 5 groups to test osteogenic differentiation: Group I: negative control, Group II: positive control, Group III: BMMSCs were treated with 1 μM AKBA, Group IV: BMMSCs were treated with 0.1 μM AKBA, and Group V: BMMSCs were treated with 0.01 μM AKBA. Mineralization assays and gene expression analysis were assessed, and the significance difference between groups was established at P < 0.05.
The flow cytometry analysis demonstrated that BMMSCs had positive expression for mesenchymal stem cell marker and negative expression for hematopoietic markers. The concentration of 0.01 μM gave significantly higher cell density than the untreated cells after 7 days (P < 0.05). Cells treated with 0.1 and 0.01 μM AKBA revealed a significantly higher ALP activity, alizarin red, and von Kossa staining than control groups (P < 0.05). High expression of osteogenic genes was detected in BMMSCs treated with 0.1 μM AKBA (P < 0.05).
It was declared that the concentration of 0.1 μM AKBA has no toxicity on BMMSC viability and proliferation with an impact on BMMSC osteogenic differentiation. Therefore, AKBA (0.01 μM) could be used in bone regeneration during periradicular surgery.
期刊介绍：
Vision SEJ aims to be one of the foremost worldwide periodical on Endodontics, dedicated to the promotion of research, post-graduate training and further education in Endodontics. Mission Statement To serve as a medium for continued Endodontic education and qualitative scientific publications on clinical trials, basic science related to the biological aspects of Endodontics, basic science related to Endodontic techniques as well as dental trauma that will ultimately improve the Endodontic research and patient’s health. Scope In this journal, Endodontists, Endodontic postgraduate students and general dentists, can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation that help them keep pace with rapid changes in this field. Aims and Objectives To publish cut edge peer-review original articles, case reports, letters to the editor, editorials, review articles, commentaries, and innovations that will impact on Endodontics. To enhance exchange of ideas/information relating to Endodontics and interaction among stakeholders. To encourage networking and partnership between individuals, government and non-governmental organizations for the provision of quality health care. To advocate for training, workshops, seminars, scientific manuscript writing conferences that will advance publishing culture.
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