Virulence factors, antifungal susceptibility and molecular profile in candida species isolated from the hands of health professionals before and after cleaning with 70% ethyl alcohol-based gel

IF 2.2 4区医学 Q3 MYCOLOGY Journal de mycologie medicale Pub Date : 2024-05-09 DOI:10.1016/j.mycmed.2024.101482

Priscila Guerino Vilela Alves , Ralciane de Paula Menezes , Nagela Bernadelli Sousa Silva , Gabriel de Oliveira Faria , Meliza Arantes de Souza Bessa , Lúcio Borges de Araújo , Paula Augusta Dias Fogaça Aguiar , Mário Paulo Amante Penatti , Reginaldo dos Santos Pedroso , Denise von Dolinger de Brito Röder

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Abstract

Fungal infections in neonatal intensive care units (NICU) are mainly related to Candida species, with high mortality rates. They are predominantly of endogenous origin, however, cross-infection transmitted by healthcare professionals' hands has occurred. The aim of this study was to identify Candida species isolated from the hands of healthcare professionals in a NICU before and after hygiene with 70% ethanol-based gel and evaluate virulence factors DNase, phospholipase, proteinase, hemolysin, biofilm biomass production, and metabolic activity. In vitro antifungal susceptibility testing and similarity by random amplified polymorphic DNA (RAPD) were also performed. C. parapsilosis complex was the most frequent species (57.1%); all isolates presented at least one virulence factor; three isolates (Candida parapsilosis complex) were resistant to amphotericin B, two (Candida famata [currently Debaryomyces hansenii] and Candida guilliermondii [currently Meyerozyma guilliermondii]) was resistant to micafungin, and six (Candida parapsilosis complex, Candida guilliermondii [=Meyerozyma guilliermondii], Candida viswanathi, Candida catenulata [currently Diutina catenulata] and Candida lusitaniae [currently Clavispora lusitaniae]) were resistant to fluconazole. Molecular analysis by RAPD revealed two clusters of identical strains that were in the hands of distinct professionals. Candida spp. were isolated even after hygiene with 70% ethanol-based gel, highlighting the importance of stricter basic measures for hospital infection control to prevent nosocomial transmission.

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用 70%乙醇凝胶清洗前后从医务人员手部分离出的念珠菌的致病因子、抗真菌敏感性和分子特征

新生儿重症监护室（NICU）中的真菌感染主要与念珠菌有关，死亡率很高。这些真菌主要来自内源性感染，但也有通过医护人员的手传播的交叉感染。本研究的目的是鉴定从新生儿重症监护室医护人员双手中分离出的白色念珠菌，并评估毒力因子 DNase、磷脂酶、蛋白酶、溶血素、生物膜生物量生成和代谢活性。此外，还进行了体外抗真菌药敏试验和随机扩增多态性 DNA（RAPD）相似性试验。副丝状菌复合体是最常见的菌种（57.1%）；所有分离株都至少有一种致病因子；3 个分离株（副丝状念珠菌复合体）对两性霉素 B 耐药，2 个分离株（家庭念珠菌[现为 Debaryomyces hansenii] 和 Guilliermondii 念珠菌[现为 Meyerozyma guilliermondii]）对米卡芬净耐药、而六种（复合副丝状念珠菌、Candida guilliermondii [=Meyerozyma guilliermondii]、Candida viswanathi、Candida catenulata [现为 Diutina catenulata] 和 Candida lusitaniae [现为 Clavispora lusitaniae]）对氟康唑有抗药性。通过 RAPD 进行的分子分析表明，有两组相同的菌株分别属于不同的专业人员。即使在使用 70% 的乙醇凝胶进行卫生处理后，仍能分离出念珠菌属，这突出表明了医院感染控制方面采取更严格的基本措施以防止院内传播的重要性。

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来源期刊

Journal de mycologie medicale 医学-真菌学

CiteScore

5.10

自引率

2.80%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal de Mycologie Medicale / Journal of Medical Mycology (JMM) publishes in English works dealing with human and animal mycology. The subjects treated are focused in particular on clinical, diagnostic, epidemiological, immunological, medical, pathological, preventive or therapeutic aspects of mycoses. Also covered are basic aspects linked primarily with morphology (electronic and photonic microscopy), physiology, biochemistry, cellular and molecular biology, immunochemistry, genetics, taxonomy or phylogeny of pathogenic or opportunistic fungi and actinomycetes in humans or animals. Studies of natural products showing inhibitory activity against pathogenic fungi cannot be considered without chemical characterization and identification of the compounds responsible for the inhibitory activity. JMM publishes (guest) editorials, original articles, reviews (and minireviews), case reports, technical notes, letters to the editor and information. Only clinical cases with real originality (new species, new clinical present action, new geographical localization, etc.), and fully documented (identification methods, results, etc.), will be considered. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey platforms.

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