HEPATOPROTECTIVE ROLE OF ALOE VERA JUICE ON ANTITUBERCULOSIS DRUGS ETHIONAMIDE AND PARA AMINO SALICYLIC ACID INDUCED HEPATOTOXICITY IN SPRAGUE-DAWLEY RATS

Abstract

Fresh Aloe vera plant leaves were brought from botanical garden and sample was indentied and brought to the laboratory in the Department of Zoology, Patkar-Varde College, Goregaon (W), Mumbai. 50 grams of leaves were then grounded with 50ml of distilled water in sterilized pestle and mortar. The yield will be calculated based on weight of the extract compared to the weight of the pulp of the leaves. Forty eight (48) Sprague- dawley rats (average weight 150 - 250 g) of either sex were used for the experiment. The drugs ETH and PAS drug and Aloe vera juice were given to respective groups daily for 28 days. At the end of study various biochemical parameters were analyzed from serum such as, Bilirubin, ACP, ALP, SGOT (AST), SGPT (ALT), HDL Cholesterol, Total Cholesterol, and TGL. Liver tissues were analyzed for Histopathology. The data was statistically analyzed by one way analysis of variance (ANOVA). The value p< 0.05 considered as signicant. Administration ETH and PAS in Sprague-dawley rats showed hepatotoxicity in test groups which was conrmed by biochemical parameters and histoarchitecture examination of liver. The rats orally administered of Aloe vera juice in combination with the ETH and PAS or independently found to be effective in lowering the elevated levels of bilirubin, ACP, ALP, ALT, AST, HDL to approximate normal level in ETH and PAS treated rats and by elevating the level of TGL. From the histopathalogical study it was also found that ETH and PAS could induce hepatitis through increases in oxidative stress, liver injury, and liver histopathology. Aloe vera juice in acts as hepato-protective agent and recovered the liver to its normal architecture. Thus current research implies the functional potential of Aloe vera juice in hepatotoxicity induced rats.