Biodegradation of nitenpyram (neonicotinoid insecticide) by endophytic bacterium, Bacillus thuringiensis strain NIT-2, isolated from neonicotinoid-treated plant samples

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY Journal of Pesticide Science Pub Date : 2024-05-17 DOI:10.1584/jpestics.d24-002
Md. Tareq Bin Salam, Koji Ito, Ryota Kataoka
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Abstract

Nitenpyram (neonicotinoid insecticide) is commonly used for crop protection from pests. Currently, due to its widespread use, the nitenpyram accumulation in the environment is anticipated to be high. Hence, the removal of nitenpyram residue from the environment is essential. However, the biodegradation of nitenpyram by endophytes is still unreported. Therefore, we aimed to isolate and identify a bacterial strain capable of degrading nitenpyram. We isolated approximately 300 endophytic strains from Brassica rapa var. perviridis that had been exposed to different neonicotinoid insecticides. After 14 days of incubation, a bacterial strain, NIT-2, with nitenpyram degradation capability (approximately 65%) was found. Via 16S rRNA gene sequencing, the strain was identified as Bacillus thuringiensis. In addition, metabolites, 2-[N-(6-chloro-3-pyridylmethyl)-N-ethyl]amino-2-methyliminoacetic acid, N-(6-chloro-3-pyridilmethyl)-N-ethyl-N-methylformamidine (CPMF), and N-(6-chloro-3-pyridilmethyl)-N-ethylformamide (CPF), were identified during the degradation. Moreover, CPMF and CPF were further degraded 71% and 18%, respectively by NIT-2. Thus, B. thuringiensis strain NIT-2 is the first reported endophytic bacterium capable of degrading nitenpyram.

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从新烟碱处理过的植物样本中分离出的内生细菌苏云金芽孢杆菌 NIT-2 株对硝虫嗪(新烟碱类杀虫剂)的生物降解作用
硝虫嗪(新烟碱类杀虫剂)常用于保护作物免受害虫侵害。目前,由于其广泛使用,硝虫酰胺在环境中的累积量预计会很高。因此,必须清除环境中的硝虫酰胺残留物。然而,内生菌对硝虫酰胺的生物降解仍未见报道。因此,我们的目标是分离和鉴定一种能够降解硝虫酰胺的细菌菌株。我们从暴露于不同新烟碱类杀虫剂的 Brassica rapa var. perviridis 中分离出约 300 株内生菌株。经过 14 天的培养,我们发现了一种具有硝虫酰胺降解能力(约 65%)的细菌菌株 NIT-2。通过 16S rRNA 基因测序,确定该菌株为苏云金芽孢杆菌。此外,在降解过程中还发现了代谢产物 2-[N-(6-氯-3-吡啶基甲基)-N-乙基]氨基-2-甲基亚氨基乙酸、N-(6-氯-3-吡啶基甲基)-N-乙基-N-甲基甲脒 (CPMF) 和 N-(6-氯-3-吡啶基甲基)-N-乙基甲酰胺 (CPF)。此外,NIT-2 对 CPMF 和 CPF 的降解率分别为 71% 和 18%。因此,苏云金芽孢杆菌菌株 NIT-2 是首个报道的能够降解硝虫酰胺的内生细菌。
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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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