Achieving temperature stability for storage of biological samples in an autodefrost freezer.

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES American journal of veterinary research Pub Date : 2024-05-20 DOI:10.2460/ajvr.24.02.0042
Jennifer J Shults, David R Bristol, Evelyn L Orenbuch
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Abstract

Objective: To assess the temperature stability of an autodefrost freezer commonly used in veterinary practices, whether the use of a Styrofoam cooler within the freezer provides temperature stability, and the ease of use of a remote monitoring system for the notification of temperature elevations.

Animals: None.

Methods: Temperature in the freezer and 2 Styrofoam coolers were assessed with remote monitoring thermometers every 15 minutes. Temperature values were monitored from October 11 to December 18, 2023 (for a 68-day period). Data analysis focused on temperatures for the freezer exceeding 0 °C and the elevations in temperatures within the coolers relative to the freezer.

Results: The freezer had an increase in temperature approximately every 16 hours. Over 68 days, the freezer had a temperature greater than 0 °C 27 times, representing 26 separate elevations. The Styrofoam coolers within the freezer never registered a temperature higher than -5 °C. Elevations in temperature within the freezer were larger in magnitude than temperature elevations within the coolers, which showed smaller-magnitude changes in temperature.

Clinical relevance: The temperature stability provided by the Styrofoam cooler would avoid potential freeze-thaw cycles of any stored biological samples. Additionally, the remote temperature monitoring system is easy to install and monitor, providing peace of mind to practice management.

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实现生物样本在自动解冻冰柜中储存的温度稳定性。
目的评估兽医诊所常用的自动解冻冰柜的温度稳定性,冰柜内使用泡沫塑料冷却器是否能提供稳定的温度,以及远程监控系统在通知温度升高方面的易用性:动物:无:方法:使用远程监控温度计每 15 分钟对冷冻室和 2 个泡沫塑料冷却器中的温度进行一次评估。监测温度值的时间为 2023 年 10 月 11 日至 12 月 18 日(共 68 天)。数据分析的重点是冷藏室温度超过 0 °C,以及冷却器内相对于冷藏室的温度升高情况:结果:冷冻室的温度大约每 16 小时上升一次。在 68 天内,冷藏室温度超过 0 °C 的次数为 27 次,分别为 26 次。冷藏室内的泡沫塑料冷却器的温度从未超过-5 °C。冷藏室内的温度升高幅度大于冷却器内的温度升高幅度,而冷却器内的温度变化幅度较小:临床意义:泡沫塑料冷却器提供的温度稳定性可避免任何储存生物样本可能出现的冻融循环。此外,远程温度监控系统易于安装和监控,可让临床管理人员放心。
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来源期刊
CiteScore
1.70
自引率
10.00%
发文量
186
审稿时长
3 months
期刊介绍: The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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