Energy deprivation affects nitrogen assimilation and fatty acid biosynthesis leading to leaf chlorosis under waterlogging stress in the endangered Abies koreana.

IF 3.5 2区 农林科学 Q1 FORESTRY Tree physiology Pub Date : 2024-06-03 DOI:10.1093/treephys/tpae055
Umashankar Chandrasekaran, Sanghee Park, Kunhyo Kim, Siyeon Byeon, Ah Reum Han, Young-Sang Lee, Neung-Hwan Oh, Haegeun Chung, Hyeyeong Choe, Hyun Seok Kim
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Abstract

Energy deprivation triggers various physiological, biochemical and molecular changes in plants under abiotic stress. We investigated the oxidative damages in the high altitude grown conifer Korean fir (Abies koreana) exposed to waterlogging stress. Our experimental results showed that waterlogging stress led to leaf chlorosis, 35 days after treatment. A significant decrease in leaf fresh weight, chlorophyll and sugar content supported this phenotypic change. Biochemical analysis showed a significant increase in leaf proline, lipid peroxidase and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical content of waterlogged plants. To elucidate the molecular mechanisms, we conducted RNA-sequencing (RNA-seq) and de novo assembly. Using RNA-seq analysis approach and filtering (P < 0.05 and false discovery rate <0.001), we obtained 134 unigenes upregulated and 574 unigenes downregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis placed the obtained differentially expressed unigenes in α-linoleic pathway, fatty acid degradation, glycosis, glycolipid metabolism and oligosaccharide biosynthesis process. Mapping of unigenes with Arabidopsis using basic local alignment search tool for nucleotides showed several critical genes in photosynthesis and carbon metabolism downregulated. Following this, we found the repression of multiple nitrogen (N) assimilation and nucleotide biosynthesis genes including purine metabolism. In addition, waterlogging stress reduced the levels of polyunsaturated fatty acids with a concomitant increase only in myristic acid. Together, our results indicate that the prolonged snowmelt may cause inability of A. koreana seedlings to lead the photosynthesis normally due to the lack of root intercellular oxygen and emphasizes a detrimental effect on the N metabolic pathway, compromising this endangered tree's ability to be fully functional under waterlogging stress.

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能量匮乏会影响濒危的韩国赤松在水涝胁迫下的氮同化和脂肪酸生物合成,导致叶片萎黄。
在非生物胁迫下,能量匮乏会引发植物的各种生理、生化和分子变化。我们研究了暴露于水涝胁迫的高海拔针叶树的氧化损伤。实验结果表明,水涝胁迫导致处理后 35 天(DAT)叶片萎黄。叶片鲜重、叶绿素和糖分含量的明显减少支持了这一表型变化。生化分析表明,受涝植物的叶片脯氨酸、脂质过氧化物酶和 DPPH 自由基含量显著增加。为了阐明其分子机制,我们进行了 RNA 测序和从头组装。利用 RNA-Seq 分析方法和过滤(P
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来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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