Su(Hw) interacts with Combgap to establish long-range chromatin contacts.

IF 4.2 2区 生物学 Q1 GENETICS & HEREDITY Epigenetics & Chromatin Pub Date : 2024-05-21 DOI:10.1186/s13072-024-00541-x
Nadezhda E Vorobyeva, Alexey N Krasnov, Maksim Erokhin, Darya Chetverina, Marina Mazina
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Abstract

Background: Insulator-binding proteins (IBPs) play a critical role in genome architecture by forming and maintaining contact domains. While the involvement of several IBPs in organising chromatin architecture in Drosophila has been described, the specific contribution of the Suppressor of Hairy wings (Su(Hw)) insulator-binding protein to genome topology remains unclear.

Results: In this study, we provide evidence for the existence of long-range interactions between chromatin bound Su(Hw) and Combgap, which was first characterised as Polycomb response elements binding protein. Loss of Su(Hw) binding to chromatin results in the disappearance of Su(Hw)-Combgap long-range interactions and in a decrease in spatial self-interactions among a subset of Su(Hw)-bound genome sites. Our findings suggest that Su(Hw)-Combgap long-range interactions are associated with active chromatin rather than Polycomb-directed repression. Furthermore, we observe that the majority of transcription start sites that are down-regulated upon loss of Su(Hw) binding to chromatin are located within 2 kb of Combgap peaks and exhibit Su(Hw)-dependent changes in Combgap and transcriptional regulators' binding.

Conclusions: This study demonstrates that Su(Hw) insulator binding protein can form long-range interactions with Combgap, Polycomb response elements binding protein, and that these interactions are associated with active chromatin factors rather than with Polycomb dependent repression.

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Su(Hw)与 Combgap 相互作用,建立长程染色质接触。
背景:绝缘体结合蛋白(IBPs)通过形成和维持接触域而在基因组结构中发挥关键作用。虽然已经描述了几种 IBPs 参与组织果蝇染色质结构的情况,但毛翅抑制因子(Su(Hw))绝缘体结合蛋白对基因组拓扑结构的具体贡献仍不清楚:在这项研究中,我们提供了证据,证明染色质结合的 Su(Hw) 与 Combgap 之间存在长程相互作用。失去 Su(Hw) 与染色质的结合会导致 Su(Hw)-Combgap 长程相互作用的消失,以及与 Su(Hw) 结合的基因组位点子集之间空间自我相互作用的减少。我们的研究结果表明,Su(Hw)-Combgap长程相互作用与活跃的染色质有关,而不是与Polycomb定向抑制有关。此外,我们还观察到,当苏木素(Hw)失去与染色质的结合时,大多数转录起始位点会被下调,这些位点位于Combgap峰的2 kb范围内,并表现出Combgap和转录调节因子结合的苏木素(Hw)依赖性变化:本研究表明,Su(Hw)绝缘体结合蛋白可与多聚酶反应元件结合蛋白Combgap形成长程相互作用,这些相互作用与活跃的染色质因子有关,而不是与多聚酶依赖性抑制有关。
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来源期刊
Epigenetics & Chromatin
Epigenetics & Chromatin GENETICS & HEREDITY-
CiteScore
7.00
自引率
0.00%
发文量
35
审稿时长
1 months
期刊介绍: Epigenetics & Chromatin is a peer-reviewed, open access, online journal that publishes research, and reviews, providing novel insights into epigenetic inheritance and chromatin-based interactions. The journal aims to understand how gene and chromosomal elements are regulated and their activities maintained during processes such as cell division, differentiation and environmental alteration.
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