Resveratrol-coated chitosan mats promote angiogenesis for enhanced wound healing in animal model

IF 2.2 3区医学 Q3 ENGINEERING, BIOMEDICAL Artificial organs Pub Date : 2024-05-23 DOI:10.1111/aor.14759

Asma Moghaddam, Fereshteh Nejaddehbashi, Mahmoud Orazizadeh

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Abstract

Background

Growing incidences of chronic wounds recommend the development of optimal therapeutic wound dressings. Electrospun nanofibers have been considered to show potential wound healing properties when accompanied by other wound dressing materials. This study aimed to explore the potential role of Chitosan (CS) nanofibrous mats coated with resveratrol (RS) as an antioxidant and pro-angiogenic agent in rat models of skin wound healing.

Methods

Electrospun chitosan/polyethylene oxide (PEO) nanofibers were prepared using electrospinning technology and coated by 0.05 and 0.1 mg.ml resveratrol named as (CS/RS 0.05) and (CS/RS 0.1), respectively. The scaffolds were characterized physiochemically such as in vitro release study, TGA, FTIR spectroscopy analysis, biodegradability, and human dermal fibroblast seeding assay. The scaffold was subsequently used in vivo as a skin substitute on a rat skin wound model.

Results

In vitro tests revealed that all scaffolds promoted cell adhesion and proliferation. However, more cell viability was observed in CS/RS 0.1 scaffold. The biocompatibility of the scaffolds was validated by MTT assay, and the results did not show any toxic effects on human dermal fibroblasts. It was observed that RS-coated scaffolds had the ability to release RS in a controlled manner. In in vivo tests CS/RS 0.1 scaffold had the greatest impact on the healing process by improving the neodermis formation and modulated inflammation in wound granulation tissue. Histological analysis revealed enhanced vascular endothelial growth factor expression, epithelialization and increased depth of wound granulation tissue.

Conclusions

The RS-coated CS/PEO nanofibrous scaffold accelerates wound healing and may be useful as a dressing for cell transfer and clinical skin regeneration.

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涂有藜芦醇的壳聚糖垫可促进血管生成，从而增强动物模型的伤口愈合能力。

背景：慢性伤口的发病率越来越高，因此需要开发出最佳的治疗伤口敷料。电纺纳米纤维与其他伤口敷料配合使用时，被认为具有潜在的伤口愈合特性。本研究旨在探索涂有白藜芦醇（RS）的壳聚糖（CS）纳米纤维垫在大鼠皮肤伤口愈合模型中作为抗氧化剂和促血管生成剂的潜在作用：方法：利用电纺丝技术制备了壳聚糖/聚环氧乙烷（PEO）纳米纤维，并在其上涂覆了 0.05 和 0.1 mg.ml 的白藜芦醇，分别命名为（CS/RS 0.05）和（CS/RS 0.1）。对支架进行了理化表征，如体外释放研究、热重分析、傅立叶变换红外光谱分析、生物降解性和人体真皮成纤维细胞播种试验。随后，在大鼠皮肤伤口模型上将该支架用作体内皮肤替代物：体外测试表明，所有支架都能促进细胞粘附和增殖。然而，在 CS/RS 0.1 支架中观察到的细胞存活率更高。MTT 试验验证了支架的生物相容性，结果显示支架对人真皮成纤维细胞没有任何毒性作用。据观察，涂有 RS 的支架能够以可控的方式释放 RS。在体内测试中，CS/RS 0.1 支架对愈合过程的影响最大，它改善了伤口肉芽组织中新真皮的形成并调节了炎症。组织学分析表明，血管内皮生长因子的表达、上皮化和伤口肉芽组织的深度都得到了增强：结论：RS 涂层 CS/PEO 纳米纤维支架可加速伤口愈合，可用作细胞转移和临床皮肤再生的敷料。

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来源期刊

Artificial organs 工程技术-工程：生物医学

CiteScore

4.30

自引率

12.50%

发文量

303

审稿时长

4-8 weeks

期刊介绍： Artificial Organs is the official peer reviewed journal of The International Federation for Artificial Organs (Members of the Federation are: The American Society for Artificial Internal Organs, The European Society for Artificial Organs, and The Japanese Society for Artificial Organs), The International Faculty for Artificial Organs, the International Society for Rotary Blood Pumps, The International Society for Pediatric Mechanical Cardiopulmonary Support, and the Vienna International Workshop on Functional Electrical Stimulation. Artificial Organs publishes original research articles dealing with developments in artificial organs applications and treatment modalities and their clinical applications worldwide. Membership in the Societies listed above is not a prerequisite for publication. Articles are published without charge to the author except for color figures and excess page charges as noted.