Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome.

IF 4.1 3区 生物学 Q2 CELL BIOLOGY Microbial Cell Pub Date : 2024-05-22 eCollection Date: 2024-01-01 DOI:10.15698/mic2024.05.823
Anusha Chaudhuri, Soumita Paul, Mayukh Banerjea, Biswadip Das
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Abstract

In Saccharomyces cerevisiae, polyadenylated forms of mature (and not precursor) small non-coding RNAs (sncRNAs) those fail to undergo proper 3'-end maturation are subject to an active degradation by Rrp6p and Rrp47p, which does not require the involvement of core exosome and TRAMP components. In agreement with this finding, Rrp6p/Rrp47p is demonstrated to exist as an exosome-independent complex, which preferentially associates with mature polyadenylated forms of these sncRNAs. Consistent with this observation, a C-terminally truncated version of Rrp6p (Rrp6p-ΔC2) lacking physical association with the core nuclear exosome supports their decay just like its full-length version. Polyadenylation is catalyzed by both the canonical and non-canonical poly(A) polymerases, Pap1p and Trf4p. Analysis of the polyadenylation profiles in WT and rrp6-Δ strains revealed that the majority of the polyadenylation sites correspond to either one to three nucleotides upstream or downstream of their mature ends and their poly(A) tails ranges from 10-15 adenylate residues. Most interestingly, the accumulated polyadenylated snRNAs are functional in the rrp6-Δ strain and are assembled into spliceosomes. Thus, Rrp6p-Rrp47p defines a core nuclear exosome-independent novel RNA turnover system in baker's yeast targeting imperfectly processed polyadenylated sncRNAs that accumulate in the absence of Rrp6p.

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酿酒酵母中的多腺苷酸化小非编码 RNA 可由 Rrp6p/Rrp47p 降解,与核心核外泌体无关。
在酿酒酵母(Saccharomyces cerevisiae)中,成熟(而非前体)小非编码 RNA(sncRNA)的多腺苷酸化形式如果没有经过适当的 3'-end 成熟,就会被 Rrp6p 和 Rrp47p 主动降解,而这种降解不需要核心外泌体和 TRAMP 成分的参与。与这一发现一致的是,Rrp6p/Rrp47p 被证明是一种独立于外泌体的复合物,它优先与这些 sncRNA 的成熟多聚腺苷酸化形式结合。与这一观察结果一致的是,Rrp6p 的 C 端截短版本(Rrp6p-ΔC2)缺乏与核心核外泌体的物理联系,它与全长版本一样支持这些 sncRNA 的衰变。聚腺苷酸化由规范和非规范聚(A)聚合酶 Pap1p 和 Trf4p 催化。对 WT 菌株和 rrp6-Δ 菌株的多聚腺苷酸化图谱进行分析后发现,大多数多聚腺苷酸化位点位于成熟末端上游或下游的一至三个核苷酸处,其 poly(A) 尾部有 10-15 个腺苷酸残基。最有趣的是,积累的多腺苷酸化 snRNA 在 rrp6-Δ 菌株中具有功能,并能组装成剪接体。因此,Rrp6p-Rrp47p 在面包酵母中定义了一种不依赖于核外泌体的新型 RNA 翻转系统,该系统的目标是在 Rrp6p 缺失时积累的未完全加工的多腺苷酸化 sncRNA。
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来源期刊
Microbial Cell
Microbial Cell Multiple-
CiteScore
6.40
自引率
0.00%
发文量
32
审稿时长
12 weeks
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