Molecular differentiation analysis of ten putative species of Fannia (Diptera: Fanniidae) collected in carrion-baited traps from Colombia.

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Medical and Veterinary Entomology Pub Date : 2024-05-25 DOI:10.1111/mve.12732
Yesica Durango-Manrique, Andrés López-Rubio, Giovan F Gómez
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Abstract

The genus Fannia is the most representative of the Fannidae family of true flies with worldwide distribution. Some species are attracted to decomposing materials and live vertebrate animals, which makes them important in forensics, medical and veterinary fields. However, identifying Fannia species can be difficult due to the high similarity in the external morphology of females and limited descriptions and morphological keys. Herein, molecular markers could provide a complementary tool for species identification. However, molecular identification has still limited application since databases contain few data for neotropical species of Fannia. This study assessed the potential of two molecular markers, the COI-3' region and internal transcribed spacer 2 (ITS2), to differentiate 10 putative species of the genus Fannia from Colombia using distance-based and tree-based approaches. The partial ITS2 and/or COI-3' regions allowed molecular diagnosis of six species, while pairs of species Fannia colazorrensis + F. dodgei and F. laclara + F. aburrae are conflicting. Although these results might suggest that conflicting pair species are conspecific, consistent morphological differences between males do not support this hypothesis. The lack of differentiation at the nuclear and mitochondrial molecular markers for the conflicting species may be due to incomplete evolutionary lineage separation, hybridization, or introgression events. In addition, sexual selection on male morphological traits before species-specific differences in molecular markers emerge may partially explain the results. Our study provides a valuable dataset to identify and confirm some Fannia species molecularly. Further, they could be used to associate females and immature stages with their conspecifics as a baseline to deep into their biology, ecology, distribution and potential applications in forensic and medico-veterinary entomology.

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从哥伦比亚的腐肉诱饵诱捕器中收集到的十种假定的 Fannia(双翅目:Fanniidae)的分子分化分析。
苍蝇属是苍蝇科中最具代表性的真蝇属,分布于世界各地。有些种类会被腐烂的物质和活的脊椎动物吸引,因此在法医、医学和兽医学领域非常重要。然而,由于雌蝇外部形态的高度相似性以及有限的描述和形态学关键字,鉴别 Fannia 物种十分困难。因此,分子标记可为物种鉴定提供补充工具。然而,由于数据库中关于新热带番杏属物种的数据很少,分子鉴定的应用仍然有限。本研究采用基于距离和基于树的方法,评估了 COI-3' 区域和内部转录间隔区 2(ITS2)这两个分子标记的潜力,以区分来自哥伦比亚的 10 个推定的番杏属物种。部分 ITS2 和/或 COI-3' 区域可对 6 个物种进行分子诊断,而 Fannia colazorrensis + F. dodgei 和 F. laclara + F. aburrae 两对物种则相互矛盾。尽管这些结果可能表明相互矛盾的一对物种是同种,但雄性之间一致的形态差异并不支持这一假设。冲突物种的核和线粒体分子标记缺乏分化可能是由于进化世系分离不完全、杂交或引入事件造成的。此外,在分子标记出现物种特异性差异之前,雄性形态特征上的性选择也可能部分解释了上述结果。我们的研究提供了一个宝贵的数据集,可用于分子鉴定和确认一些番杏属物种。此外,它们还可用于将雌性和未成熟阶段与同种昆虫联系起来,作为深入研究其生物学、生态学、分布以及在法医和兽医昆虫学中潜在应用的基线。
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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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